O-carboxymethyl chitosan Schiff base complexes as affinity ligands for immobilized metal-ion affinity chromatography of lysozyme

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dc.authoridALACABEY, ihsan -- 0000-0002-3080-2296
dc.contributor.authorAcet, Ömür
dc.contributor.authorBaran, Talat
dc.contributor.authorErdönmez, Demet
dc.contributor.authorAksoy, Neşe Hayat
dc.contributor.authorAlacabey, İhsan
dc.contributor.authorMenteş, Ayfer
dc.contributor.authorOdabaşı, Mehmet
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:28:14Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:28:14Z
dc.date.issued2018
dc.departmentFen-Edebiyat Fakültesi Veteriner Fakültesi
dc.description.abstractWe synthesized Ni2+-attached O-Carboxymethyl chitosan Schiff base complexes embedded composite cryogels (Ni2+-O-CMCS-CCs) by means of polymerization of gel-forming precursors at subzero temperatures. Prepared affinity cryogel showed excellent adsorption performance for lysozyme selected as model protein to test adsorption parameters, demonstrating an adsorption capacity of 244.6 mg/g (15.3 mg/g for Ni2+ minus O-CMCS-CCs), with fast adsorption equilibrium within 30 min and good reversibility. The performance of Ni2+-O-CMCS-CCs for lysozyme was also evaluated by SDS-PAGE, and a purification efficiency of 86.9% with 89.5% purification yield was determined. The swelling test, FT-IR, and SEM analysis were carried out for the characterization of Ni2+-O-CMCS-CCs. At the end of 35 adsorption-desorption cycles, there was no significant change in the adsorption capacity.
dc.description.sponsorshipAksaray University Scientific Research Projects Coordination, Turkey [2015-80]
dc.description.sponsorshipThis study was supported by Aksaray University Scientific Research Projects Coordination (Project Number: 2015-80), Turkey.
dc.identifier.doi10.1016/j.chroma.2018.03.022
dc.identifier.endpage27en_US
dc.identifier.issn0021-9673
dc.identifier.issn1873-3778
dc.identifier.pmid29609862
dc.identifier.scopusqualityQ1
dc.identifier.startpage21en_US
dc.identifier.urihttps://doi.org/10.1016/j.chroma.2018.03.022
dc.identifier.urihttps://hdl.handle.net/20.500.12451/6007
dc.identifier.volume1550en_US
dc.identifier.wosWOS:000430897400003
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofJOURNAL OF CHROMATOGRAPHY A
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCryogel
dc.subjectIMAC
dc.subjectCarboxymethyl Chitosan
dc.subjectLysozyme Adsorption
dc.titleO-carboxymethyl chitosan Schiff base complexes as affinity ligands for immobilized metal-ion affinity chromatography of lysozyme
dc.typeArticle

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