Comparing ethylene glycol with glycerol and with or without dithiothreitol and sucrose for cryopreservation of bull semen in egg-yolk containing extenders

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dc.authoridDurmaz, Emre -- 0000-0003-2239-1762; TUNCER, Purhan Barbaros -- 0000-0002-9257-9544
dc.contributor.authorBüyükleblebici, Serhat
dc.contributor.authorTuncer, Pürhan Barbaros
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorTaşdemir, Umut
dc.contributor.authorEken, Ayşe
dc.contributor.authorBüyükleblebici, Olga
dc.contributor.authorDurmaz, Emre
dc.contributor.authorSarıözkan, Serpil
dc.contributor.authorEndirlik, Burcu Ünlü
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:28:25Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:28:25Z
dc.date.issued2014
dc.departmentVeterinerlik Fakültesi
dc.description.abstractThere are few studies performed for investigating the roles of different ratio and cryoprotectants with dithiothreitol or sucrose on sperm motility characteristics and antioxidant capacities of post-thawed bull spermatozoa. The objectives of this study were to compare glycerol (G) and ethylene glycol (EG) at different concentrations as cryoprotectants and dithiothreitol (D) or sucrose (S) (with/without) as antioxidants in Tris extender for cryopreservation of bull semen. Twenty-four ejaculates obtained from three bulls were included in the study. Each ejaculate was split into four equal aliquots and diluted using both of the Tris extenders with glycerol (5% or 7%) or ethylene glycol (3% or 5%). After that, each extenders were split into three equal aliquots and diluted using both of the dithiothreitol 5 mM or sucrose 25 mM, and control (without additives) was cooled to 4 degrees C and frozen in 0.25-ml French straws. when compared to control, different doses cryoprotectants and antioxidants addition no significantly increased the percentages of post-thaw sperm progressive and motitilities, acrosome abnormality and plasma membrane integrity (P > 0.05). However, EG3 + S yielded the greatest percentages of the total abnormality (P < 0.05). As regard to antioxidant activities G7 and EG5 led to lowest MDA activity with or without D or S but, these results were not supported to the GPx activity (P < 0.01). The sperm motion characteristics such as VAP, VCL, ALH and BCF gave significantly different results (P < 0.05). When compared the DNA integrity, different doses cryoprotectants without antioxidants addition significantly increased the percentages the tail intensity and tail moment (P < 0.05). There were no significant differences observed in non-return rates among all treatment groups (P > 0.05). (C) 2014 Elsevier Inc. All rights reserved.
dc.description.sponsorshipRepublic of Turkey, Ministry of Agriculture and Rural Affairs; General Directorate of Agricultural Research (GDAR) [09/01/01/01]
dc.description.sponsorshipThis study was supported by the Republic of Turkey, Ministry of Agriculture and Rural Affairs, General Directorate of Agricultural Research (GDAR) Project number: 09/01/01/01.
dc.identifier.doi10.1016/j.cryobiol.2014.05.005
dc.identifier.endpage78en_US
dc.identifier.issn0011-2240
dc.identifier.issn1090-2392
dc.identifier.issue1en_US
dc.identifier.pmid24858399
dc.identifier.scopusqualityQ1
dc.identifier.startpage74en_US
dc.identifier.urihttps://doi.org/10.1016/j.cryobiol.2014.05.005
dc.identifier.urihttps://hdl.handle.net/20.500.12451/6035
dc.identifier.volume69en_US
dc.identifier.wosWOS:000340691700012
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAcademic Press
dc.relation.ispartofCryobiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBovine
dc.subjectAntioxidants
dc.subjectCryoprotectants
dc.subjectSperm Parameters
dc.subjectFreeze-Thawing
dc.titleComparing ethylene glycol with glycerol and with or without dithiothreitol and sucrose for cryopreservation of bull semen in egg-yolk containing extenders
dc.typeArticle

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