Makale Koleksiyonu

Bu koleksiyon için kalıcı URI

https://hdl.handle.net/20.500.12451/379

Listeleniyor 1 - 20 / 147

Serum IgG threshold values associated with increased risk of diseases in preweaned lambs
(İlker ÇAMKERTEN, 2025) Gokçe, Erhan; Atakişi, Onur; Cihan, Pınar; Kırmızıgül, Ali Haydar; Erdoğan, Hidayet Metin
Serum IgG cut-off points associated with increased risk of septicemia, fatigue anorexi syndrome (FAS), diar- rhea and pneumonia in preweaned lambs was investigated in this study. The study involved 347 Akkaraman crossbred lambs born on two farms in Kars, Turkey. Blood samples were collected 24±1 h after birth and serum IgG concentrati- on was measured by ELISA assay and cut-off values for each disease were determined. Neonatal lambs with diarrhea, FAS and septicaemia had statistically significantly lower IgG concentrations compared to healthy lambs (P<0.05). Cri- tical SIgGC-24 cut-off values for increased risk of diarrhea, FAS and septicaemia in neonatal lambs were <800, <1000 and <200 mg/dl. In post-neonatal period, SIgGC-24 (mg/dL) was lower in only lambs with pneumonia compared to he- althy lambs (P<0.05). The risk of developing septicaemia (IgG<200 mg/dL vs IgG>200 mg/dL), diarrhoe (IgG<800 mg/ dL vs IgG>800 mg/dL), FAS (IgG<1000 mg/dL vs IgG>1000 mg/dL) and pneumonia ((IgG<1000 mg/dL vs IgG>1000 mg/dL) was 203, 6, 18 and 12 times higher, respectively. A threshold vaule of IgG<998 mg/dL and IgG<193 mg/ dL were determined for neonatal morbidity and mortality, respectively. An appropriate colostrum management may help to maintain the health of pre-weaning lambs, thereby improving the productivity and profitability of sheep farms..
Investigation of the effects of mesenchymal stem cell administration on liver recovery in experimental hepatotoxicity model
(İlker ÇAMKERTEN, 2022) Boztok Özgermen, Başak; Yavuz, Orhan; Haydardedeoğlu, Ali Evren
Hepatotoxicity refers to liver dysfunction associated with certain medical drugs and chemicals. Studies have shown that mesenchymal stem cells have a positive effect on the improvement of liver diseases. This study aimed to investigate the potential protective effects of fetal kidney-induced mesenchymal stem cells on Doxorubicin- induced hepatotoxicity in rats. Sprague dawley rats were divided into three groups: Control, sham, and treatment group. Intraperitoneally administered mesenchymal stem cells were treated with BrdU before transplantation so that they could be followed up after invivo transplantation. After completion of the experimental steps, the groups were monitored for 5 weeks. Then the rats were terminated and liver tissues were taken for histopathological and immunohistochemical evaluation. In immunohistochemical examinations performed with TNF-α, Caspase-3, and COX-2 primary antibodies, the most severe positivity was in the sham group, followed by the control and treatment groups. While the control and sham groups were found to be statistically similar in immunohistochemical staining with anti-BrdU antibody, the treatment group was found to be significantly different from the other groups (p<0.05). As a result, it has been revealed that intraperitoneally administered mesenchymal stem cells prevent degeneration and necrosis in hepatocytes, significantly decreasing TNF-α, COX-2, and Caspase-3 levels thus increasing liver regeneration in rats with Doxorubicin-induced hepatotoxicity.
Changes in cardiac markers in a calf died of foot and mouth disease
(İlker ÇAMKERTEN, 2022) Ayvazoğlu, Cemalettin; Gokçe, Erhan; Erdoğan, Hidayet Metin
This report disclosed changes in cardiac troponin expression during myocardial degeneration in a calf with foot and mouth disease (FMD). The case presented was a 7-day-old calf milk fed by its mother which diagnosed as having FMD (O-type) by Ministry of Agriculture and Forestry Şap (Foot and Mouth Disease) Institute. The calf suffered from myocarditis due to suckling FMD infected dam. Blood sample was taken from the jugular vein of calf. Serum cTn-T, cTn-I, CK-MB, LDH, AST and ALT levels were measured using commercial kits. cTn-T, cTn-I, CK-MB, LDH, AST and ALT levels were determined as 1.00 ng/mL, 26.68 ng/mL, 262 U/L, 4503 U/L, 56.6 U/L, 55.3 U/L, respectively and these values were higher than those of healthy calf. It is thought that the use of cardiac troponins (cTn-I, cTn-T) for the diagnosis of the disease will give accurate and rapid results.
The influence of blood, seminal plasma testosterone, growth hormone and cortisol levels on the sperm quality in merino rams
(İlker ÇAMKERTEN, 2022) Ergin Eğritağ, Hale; Özgökçen, Nagehan; Harman, Halil; Güngör, Şükrü; Öztürk, Caner; Serpek, Behiç
The research was conducted on 10 Merino rams to determine the effect of testosterone, growth hormone and cortisol levels on the sperm quality. Beginning in January, blood samples were taken from the jugular veins of rams every month, centrifuged for 20 min at 3000 rpm and obtained plasma. On the days the blood samples were taken, the semen samples which were collected with an artificial vagina, were divided between two eppendorf tubes, one of which was used for the determination of the semen characteristics, while the other was centrifuged to obtain seminal plasma. A significant relationship was detected between blood and seminal plasma levels of testosterone (P˂0.01). No growth hormone (GH) on a measurable level was detected in the seminal plasma, and it was determined that plasma GH levels, just as testosterone levels began to increase with the approach of the reproductive season, and peaked at the start of the season. Plasma and seminal plasma cortisol levels, on the other hand, while increasing before the season, decreased significantly at the start of the season, and a remarkable correlation between plasma and seminal plasma levels was ascertained (P˂0.01). An important negative relation which was observed between plasma GH levels and seminal plasma cortisol levels in Merino rams. It was found out that the hormones that were examined did not have a significant influence on sperm qualities, but there was an important negative relation only between the plasma GH levels and sperm volumes in the Merino (P˂0.01). In conclusion, it may be stated that in rams, especially plasma testosterone and cortisol levels determine the plasma levels; both plasma and seminal plasma testosterone levels increase to a significant degree in the reproductive season. It can be said that there is no significant relationship between semen quality and plasma and seminal plasma hormone levels.
Pathological and biochemical investigation of the effects of L-carnitine and gemfibrozil on peroxisome proliferator activated receptors (PPARS) and lipidosis in rabbits on a high-fat diet
(İlker ÇAMKERTEN, 2022) Çıtıl, Mehmet; Tunca, Recai; Uzlu, Erdoğan; Karapehlivan, Mahmut; Adalı, Yasemen; Yapar, Kürşad; Eroğlu, Hüseyin Avni; Erkılıç, Ekin Emre; Makav, Mustafa; Erdoğan, Hidayet Metin
Obesity and fatty liver is a worldwide health problem in human with detrimental consequences where many investigations are undertaken to overcome this problem. In this study, gemfibrozil and L-carnitine were evaluated in prevention of obesity and lipidosis. The study involved 56 New-Zealand Albino rabbits, divided into 8 equal groups (n=7). The groups were as follow; group I (normal diet), II (normal diet +gemfibrozil), III (normal diet+L-carnitine) and IV (normal diet+gemfibrozil+L- carnitine), V (high fat diet), VI (high fat diet+gemfibrozil), VII (high fat diet+L- carnitine) and VIII (high fat diet+gemfibrozil+L-carnitine). Animals were blood sampled and wieght weekly during the experiment and at the end of the experiment for determination of biochemical parameters (glucose, total lipid). All rabbits were euthanised for histopathological examination and for distrubition of peroxisome proliferator activated receptors (PPARs) in tissues by immunohystochemistry. Gemfibrozil and L-carnitin treatment in rabbits given high fat diet resulted in statistically significant decrease in total lipid when compared to those only received high fat diet. Beta oxidation of high fat diet group was significantly higher than that of groups additionally received gemfibrozil and L-carnitine. Immunohistochemistry revealed an increase in PPAR, PPAR-α and β but not PPAR-γ expression in high fat diet group. On the contrary, L-carnitin administration had no effect on tissue PPAR expression. PPAR-α expression differed between groups received gemfibrozil and high fat diet and those did not. The most marked macroscopy finding was abdominal fat increase in high fat diet group (group V). On the other hand gemfibrozil administration resulted in significant abdominal fat decrease. Furthermore decreased abdominal fat was marked in gemfibrozil and L-carnitine given animals (group VIII) when compared to other groups. In conclusion, gemfibrozil and L-carnitine administration alleviated abdominal and hepatic fattening. Gemfibrozil also caused a significant increase in PPAR-α expression in the liver. It may be of use in avoiding abdominal fat (obesity) due to high fat diet by use of gemfibrozil, a synthetic PPAR-a ligand, and L-carnitine.
Kedilerde arka ekstremite travmatik lezyonlarının dağılımı ve sağaltımı üzerine klinik çalışmalar
(Van Yüzüncü Yıl Üniversitesi, 2021) Sağlam, Mehmet; Taban, Hasan Mertcan; Fadıl, Abdurrahim
Bu çalışmada, Ankara Üniversitesi Veteriner Fakültesi Hayvan Hastanesi Cerrahi Kliniğine getirilen, arka ekstremitelerinde travmatik lezyonlar bulunan farklı ırk, yaş ve cinsiyetteki 20 kedi değerlendirildi. Olgularda gerçekleştirilen klinik ve radyolojik muayeneler sonrasında; 13 olguda arka ekstremiteleri kemiklerinde kırık, 2 olguda caput femoris lüksasyonu, 2 olgu’da art. tarsi lüksasyonu, 3 olgu’da açık yara şekillenmiş olup, endike olan sağaltımları gerçekleştirilerek postoperatif kontroller sürdürülmüştür. Olguların anamnezleri ve inspeksiyon bulgularına göre sistemik muayeneleri gerçekleştirildi. Lezyon belirlenen ekstremite ağrı, sıcaklık, şişkinlik, deformite, anormal hareket, krepitasyon ve diğer ekstremitesi ile asimetri bulguları yönünden muayene edildi. Konservatif sağaltım endikasyonu bulunan kırıklarda destekli bandaj ve kısıtlı alan uygulamaları yeterli olup, rutin bakım ve kontrolleri sürdürüldü. Açık redüksiyon ve fiksasyon gerektiren olgularda endike olan sağaltım yöntemleri ve postoperatif rutin kontrolleri sürdürülerek fonksiyonel iyileşmeleri sağlandı. Klinik olguların değerlendirildiği bu çalışmada, kedilerde karşılaşılan arka ekstremite travmatik lezyonlarının genel olarak tanımı, oluşum nedenleri, lokalizasyonu ile endike olan konservatif veya operatif sağaltım yöntemlerinin uygulanmasına ilişkin ayrıntılı bilgiler aktarılmıştır.
Evaluation of functional structures in the ovaries pre and post ovulation by doppler ultrasonography in bitches
(Atatürk Üniversitesi Veteriner Fakültesi, 2021) Bulut, Gaye
In this article, functional structures on the ovaries of bitches before and after ovulation are evaluated with Doppler ultrasonography and information concerning the interpretations of findings are transferred. Doppler effect is the first step of Doppler imaging. This effect is the shift in the frequency of the transmitted ultrasound. Spectral and/or color Doppler ultrasonography is the easiest and the most important diagnostic technique in assessing ovarian hemodynamic. The Doppler shift and the Doppler angle are used by the instrument for computing blood velocity. Spectral Doppler contains quantitative information. This information includes the peak systolic volume, end-diastolic volume, and time-averaged maximum velocities, Doppler indices (pulsatility index; PI; resistance index, RI). Doppler indices are used to assess vascular perfusion and computed using values PSV, EDV, and TAMV. The presence of blood flow in a color doppler image is shown as color-encoded and superimposed on a real-time B-mode image. Color Doppler imaging provides detailed monitoring of the local blood flow in ovarian follicles and corpus luteum. The discovery of Doppler ultrasonography that includes blood flow information has led to the formation of research issues in veterinary medicine. It is a developing technology in veterinary medicine. This technology is provided an understanding of hemodynamics and the relationship between blood flow and physiological-pathophysiological processes.
Koç spermasının kısa süreli saklanmasında katkı maddelerinin etkisi
(İlker ÇAMKERTEN, 2021) Öztürk, Caner; Dursun, Şükrü; Bulut, Gaye; Karaşahin, Tahir
Bu çalışmanın amacı, kısa süreli saklanan koç spermasının (24 saatlik aralıklarla) 72 saate kadar spermatolojik parametreler üzerine etkilerini araştırmaktı. Çalışmada % motilite, canlılık ve akrozom bütünlüğü verileri değerlendirildi. Çalışmada her koçtan (4 baş Merinos koçu) alınan dört ejakülat kullanıldı. Ejakülatlar pooling yapıldıktan sonra eşit hacimli yedi parçaya bölündü. Çalışma grupları, tris bazlı sulandırıcıya eklenen resveratrol (2 ve 4 mM), troloks (2 ve 4 mM), BSA (3 ve 6 mg/ml) ve kontrol olmak üzere oluşturuldu. Sulandırılan örnekler 72. saate kadar 4°C de muhafaza edildi. Spermatozoa motilitesi, canlılık ve akrozom bütünlüğü 0, 24, 48 ve 72. saatte değerlendirildi. Motilite muayenesi faz kontrast ataçmanlı mikroskopta 400X lük büyütmede, canlılık ve akrozom bütünlüğü ise floresan mikroskop ile değerlendirildi. Çalışmanın 72. saatinde, BSA (3 ve 6mM) grupları motilite (53,75±2,50%; 55,00±4,08%) ve akrozom bütünlüğü (53,70±3,39%; 57,10±4,68%) değerlendirmelerinde kontrol grubu (43,75±2,50%; 46,53±3,58) ile istatistiksel farklılık (p<0,05) gösterdi. Troloks (4mM) grubunda (67,22±3,71%), tüm gruplara kıyasla 72. saatte en yüksek canlılık sonucuna ulaşılmış ayrıca kontrol grubu (46,53±3,53) ile istatistiksel farklılık (p<0,05) belirlenmiştir. Çalışmanın sonucunda koç sperma sulandırıcısına eklenen BSA ve troloksun koç spermasının kısa süreli saklamasında faydalı olduğu ve spermatolojik parametreler üzerine koruyucu etki gösterdiği belirlenmiştir.
The incidence of neospora caninum in dairy cows with abortion and infertility problems in Aksaray providence
(Afyon Kocatepe Üniversitesi, 2021) Bulut, Gaye; Ün, Hikmet; Sanioğlu Gölen, Gökçenur; Camkerten, İlker
The contribution of animal husbandry to the national economy cannot be ignored. The causes of infertility are various and complex. Economic losses are increasing in dairy cattle breeding due to infectious and non-infectious causes. The percentage of abortion due to infectious agents is not fully known, but infectious agents lie in about 90% of the cases whose etiology can be determined. Neospora caninum is considered to be one of the most important abortion factors of cattle. Although, this protozoa is always ignored. It causes abortion in cows. In this study, it was aimed to detect N. caninum from the blood serum obtained from 137 dairy cattle brought to Aksaray University Faculty of Veterinary Medicine between 2017-2019 and suffering from abortion and infertility problems. For serological diagnosis, ELISA (Enzyme-Linked ImmunoSorbent Assay) test was performed.As a result, N. caninum agent was detected in 37.22 % (51/137) of the samples that belong to cattle with abortion and infertility problems. It was concluded that economic loss due to infertility in dairy cattle and protozoa, which are one of the infection factors, should be given importance.
Circulating serum zonulin levels before and after probiotic enema treatment in dogs with atopic dermatitis: randomized clinical study
(Türkiye Klinikleri Yayınevi, 2021) Ural, Kerem; Erdoğan, Hasan; Erdoğan, Songül; Camkerten, İlker; Nurcan, Şahin
In the present study, we investigated the benefits of rectal enema probiotic combination in dogs with atopic dermatitis and detected circulating serum zonulin levels in these. Material and Methods: An open-label, self-controlled case series involved 6 privately owned dogs with atopic dermatitis. Diagnosis involved corneometric analytes, Canine Atopic Dermatitis Extent and Severity Index version 4 (CADESI-04) scores, visual analogue scale (VAS) pruritus scores and in vitro allergen specific tests. Faecal samples were forwarded to dual indexing one-step polymerase chain reaction and 16S rRNA targeted metagenomics in an attempt to determine gut microbiota alterations before and after rectal enema probiotic once daily for 10 days. Results: Following rectal enema probiotic treatment, as the in the vast majority Prevotella genus was significantly detected in association with clinical cure and recovery. Median CADESI-04 (p=0.028) and VAS pruritus scores (p=0.028) along with circulating zonulin (p=0.026) levels were decreased in atopic dermatitis cases on day 10 in contrast to 0th day values. Corneometric analysis involving median epidermal pH (p=0.027) and hydration values (p=0.028) revealed a significant improvement. Conclusion: This positive impact on skin inflammation and diminshed pruritus after application of rectal enema probiotic in this model of atopic dermatitis in dogs probably presented withdrawal of chronic barrier disruption and healed tigh junctions. The selected Lactobacillus probiotic strains used as an enema potentially regulate on skin health by the manipulation of the gut microbiome resulted with intestinal balance.
Diagnosis of Avian Tuberculosis in laying hens by pathological, microbiological and polymerase chain reaction (PCR): Case report
(İlker ÇAMKERTEN, 2021) Yavuz, Orhan; Özdemir, Özgür; Sayın, Zafer; Hatipoğlu, Fatih; Hadımlı, Hasan Hüseyin
Avian tuberculosis was described at 50 week-old Lohmann chickens in a commercial chicken farm. The study materials were 50 week-old 20 pieces of chicken from 2 coops and 16 week-old 20 pieces of pullets from 4 coops in a commercial chicken farm. After necropsy, samples were processed routinely for histopathological and microbiological examinations. Macroscopically, hard consistency, numerous and different sizes, whitish-yellow caseificated-calcifiated nodules were seen on the liver, spleen, kidneys and intestinal serosa. At microscopic examination, various sized granulomas, which have been caseification necrosis surrounded by epithelioid histiocytes and multinucleated giant cells, were found at liver, spleen, wall and serosa of intestine. Numerous acid-fast bacteria were seen on histopathology at necrosis and macrophages in the liver, spleen and intestines by Ziehl – Neelsen staining. Mycobacterium avium spp was produced at microbiological inoculations in liver, spleen, intestines and ovaries. Mycobacterium avium subsp. avium from these cultures was identified by PCR. It was thought that infection could be by fecal-oral route due to both intestinal tuberculosis in hens and common disease in coop. Therefore, the role of chicken manure may also be taken into account for the spreading of the disease.
Assessment of diaphyseal long bone fracture healing in cats using radiography, ultrasonography and doppler impedance index
(İlker ÇAMKERTEN, 2024) Avcı, Nihan; Ergin, İrem; Şen, Yusuf
Color Doppler ultrasonography evaluates blood flow and neovascularization in vessels, assessing Pulsatility Index (PI) and Resistive Index (RI) values. The primary objective of this study is to monitor the healing periods of fractures using color Doppler ultrasonography index values, thereby preventing complications through objective data. The emphasis is particularly on establishing the foundation for studies aimed at early detection of delays in vascularization associated with fracture healing. The research involved 20 cats of various breeds and genders, aged 1 to 4 years, diagnosed with femur and tibia diaphyseal fractures. Mediolateral and anteroposterior radiographs were taken on postoperative days 0, 10, 20, and 30. The fracture site was examined using color Doppler ultrasonography. As a result of the study, using Doppler ultrasonography and radiography, neovascular areas from fracture formation to callus formation were converted into objective data through vascular indices. Particularly noteworthy was a significant increase in RI and PI values observed on the postoperative 10th day. It was deduced that the detection of fracture healing could occur earlier with the assistance of these index values when compared to radiographs taken on the 10th and 30th days. In conclusion, PI and RI values may be important parameters in evaluating the healing process in complex fractures, especially in determining complications and monitoring the progress of healing. Moreover, Doppler indices might be employed to complement traditional diagnostic approaches in the early detection of pathological bone conditions
Feline Panleukopenia - Effects of Treatment with Filgrastim on Mortality, Hematological and Biochemical Parameters
(Universidade Federal do Rio Grande do Sul, 2025) Uyurca, Tarık; Haydardedeoğlu, Ali Evren
Panleukopenia is a highly contagious and fatal disease of cats. Although infection may be prevented by intensive vaccination of animals in the home environment, infection is frequently seen in stray animals that are not vaccinated and are in contact with the virus. Treatment protocols in the disease consist of classical supportive therapies because there is no specific antiviral drug. The aim of this study is to evaluate the effects of Filgrastim administration on mortality, complete blood count, and serum biochemical parameters in the treatment of cats infected with Feline Panleukopenia Viruses (FPV). Materials, Methods & Results: The study population consisted of 2-6 months old cats of different breeds and sexes who were brought to our clinic with complaints of lethargy, anorexia, high fever, diarrhoea and vomiting. A complete blood count was taken from the symptomatic patients during physical examination and FPV Ag test was performed. Fourteen cats with positive FPV antigen test and 7 healthy cats with negative FPV Ag test without clinical symptoms were included in the study. The study included 7 Panleukopenia-infected cats treated with Filgrastim in addition to conventional supportive therapy, 7 Panleukopenia-infected cats treated with conventional supportive therapy, and 7 healthy cats. Complete blood count and serum biochemical parameters were analyzed from the samples taken from the patients on days 0, 3 and 7. As a result of the treatment, the mortality rate was 14.28% in both groups. In this study, it was observed that the administration of Filgrastim (Group 1) had statistically significant results on WBC (P < 0.009), lymphocytes (P < 0.009) and granulocytes (P < 0.003) between days 0 and 7 in cats with FPV. This suggests that the administration of Filgrastim in addition to supportive therapy increases the number of lymphocytes and granulocytes. The administration of Filgrastim as an adjunct to supportive therapy had no effect on the serum biochemistry values of the patients. In conclusion, the administration of Filgrastim in the treatment of panleukopenia did not result in a change in the mortality rate. Discussion: The incidence of FPV decreases as the age of the animal increases. In cats aged 3-5 months, the amount of antibody from the mother decreases. In this study, it was determined that the age of all FPV Ag positive cats included in group 1 and group 2 was 2-6 months. It has been reported that there may be a rapid increase in neutrophil counts in cats with FPV treated with filgrastim. In this study, it was observed that the use of Filgrastim (Group 1) in cats with FPV produced statistically significant results on WBC, lymphocytes and granulocytes between days 0 and 7. This shows that the use of Filgrastim in addition to supportive therapy increases the number of lymphocytes and granulocytes. The incidence of FPV decreases as the age of the animal increases. In cats aged 3-5 months, the amount of antibody from the mother decreases. In this study, it was determined that the age of all FPV Ag positive cats included in group 1 and group 2 was 2-6 months. Serum biochemistry parameters are not specific for FPV. Hypoalbuminaemia is the most common abnormality, probably due to decreased protein intake and leakage from mucosal lesions into the gastrointestinal tract. Similar to the literature, no significant change was observed in biochemical parameters. In addition, serum biochemical examinations have shown that Filgrastim can be administered to cats without any side effects. Before administering an immune modulator such as G-CSF, the stage of the disease and the status of the immune system should be taken into consideration.
Diagnostic importance of serum neopterin, procalcitonin and some acute phase proteins in cattle with lung hydatid cyst
(Hellenic Veterinary Medical Society, 2025) Akyüz, E.; Aydın, U.; Merhan, O.; Karakurt, E.; Sezer, M.; Batı, Y.U.; Yıldız, U.; Nuhoğlu, H.; Erkılıç, E.E.; Ölmez, N.; Özaydın, İ.; Gökçe, G.; Erdoğan, H.M.
In this study, it was aimed to determine the diagnostic importance of serum neopterin, procalcitonin, and some acute phase proteins in cattle with lung hydatid cysts. In the study, 30 cattle aged 2-8 years, Simmental or crossbred, constituted the patient group, and 10 healthy cattle in the same characteristics and age range constituted the control group. The cattle were included in the study after being diagnosed with lung hydatid cyst as a result of clinical, anamnesis, auscultation, radiographic examinations, laboratory and histopathological findings. Neopterin (21.60 nmol/L) and procalcitonin concentrations (146.77 ng/L) were significantly increased in the disease group compared to the control group (respectively: 7.22 nmol/L, 47.76 ng/L) (P<0.001). Haptoglobin (P<0.001) and ceruloplasmin (P=0.047) of the acute phase proteins werehigher in the patient group compared to the control group, while albumin was found to be lower (P=0.028). In conclusion, the evaluation of serum neopterin, procalcitonin, and some acute phase proteins in cattle with lung hydatid cysts was found to be important. Since hydatid disease is a zoonotic disease, it will be important to perform radiography scans and confirm the diagnosis with relevant biomarkers and take new protective measures. We think that the diagnosis will be strengthened by investigating neopterin and procalcitonin levels, especially in cattle with suspected lung cysts in radiographic findings.
Radiographic cardiac indices for healthy New Zealand white rabbits: A reference interval study based on echocardiography
(Ankara University, 2025) Kaya, Mahir; Çetinkaya, Mehmet Alper
This research was intended to identify reference intervals for the radiographic cardiac indices [vertebral heart scale (VHS), radiographic left atrial dimension (RLAD), and vertebral left atrial size (VLAS) of 58 healthy, adult New Zealand white rabbits based on echocardiography. The VHS, VLAS, and RLAD measurements were taken from contrast right lateral (R) and ventrodorsal (VD) thoracic radiographs. The correlations between these radiographic cardiac indices and echocardiographic parameters were then evaluated. The mean values with a reference interval were 7.94±0.31 vertebrae (v) (7.2-8.6 v) for R-VHS and 8.67±0.33 v (7.8-9.2 v) for VD-VHS. The median values with a reference interval were 1.5 v (1-2 v) for VLAS and 1 v (0.7-1.4 v) for RLAD. Body weight and gender had no effect on radiographic cardiac indices. There were positive correlations between all radiographic indices obtained from the R contrast radiographs and the echocardiographic parameters (rs≥0.421, P<0.0001). Excellent intraobserver agreement was determined for the radiographic measurement methods (intraclass correlation coefficients ≥0.818). The contrast thoracic radiography appears to represent a useful technique for the accurate determination of radiographic cardiac indices. The findings can be used as reference values for radiographic cardiac evaluation in both pet and laboratory rabbits.
Prevalence and Molecular Characterization of Moniezia Species in Ruminants Based on ITS1-5.8S rRNA from Van Province, Turkey
(University of Agriculture, 2025) Yılmaz, Ali Bilgin; Azizoğlu, Erkan; Adizel, Özdemir; Göz, Yaşar; Çelik, Burcak Aslan; Çelik, Özgür Yaşar; Ayan, Özge Oktay; Akhmetzhanova, Aizhan; Ayan, Adnan; Yasul, Muhammed; Myrzhiyeva, Assem; Uslu, Uğur
This study aimed to calculate the occurence of Moniezia species in cattle, sheep, and goats in Van province and to identify these species using morphological and molecular methods (ITS1-5.8S rRNA gene region analysis). Additionally, the study aimed to identify the genetic differences between Moniezia expansa and Moniezia benedeni. During the summer of 2022, intestinal contents were collected from 150 ruminants (50 cattle, 50 sheep, and 50 goats) slaughtered in slaughterhouses in Van province. The parasites were examined using Aceto-Carmine staining, and species identification was based on interproglottidal glands. Examination of the intestinal contents revealed that 2 out of 50 cattle (4%), 14 out of 50 sheep (28%), and 9 out of 50 goats (18%) were infected with Moniezia. Morphological and molecular analyses showed that the cattle samples were identified as Moniezia benedeni, goats samples as Moniezia expansa and those from sheep as 11 Moniezia expansa and 3 Moniezia benedeni. Following DNA extraction, the ITS1-5.8S rRNA gene region was amplified using PCR and subjected to sequence analysis. The relationship between species was examined by phylogenetic tree. This study confirms the prevalence of Moniezia spp. in Van/Türkiye by using the ITS1-5.8S rRNA gene.
Estrus synchronization using progesterone+different gonadotropins or flushing+ram effect in the breeding season in sheep
(Biblioteca Universidad de Cordoba, 2025) Kırbaş, Mesut; Köse, Mehmet; Bülbül, Bülent; Dursun, Şükrü; Demirci, Uğur; Erduran, Hakan; Sarı, Deniz
. The effect of different doses of Equine Chorionic Gonadotropin (eCG)or a dose of folliclestimulating hormone (FSH) in a progesterone-based estrus synchronization protocol or flushing+ram effect on fertility in the breeding season were examined on Konya Merino ewes. Materials and methods. Ewes were divided into six groups. Estrus of ewes in eCG-300, eCG-500, eCG-700, and FSH-10 groups was synchronized with an intravaginal sponge containing progesterone (Chronogest, 20 mg flugestone acetate, Intervet, Türkiye 20 mg)-derived for 12 days, and PGF2α injected one day before sponge removal. eCG-300, eCG-500, and eCG-700 groups received 300, 500, or 700 IU of eCG, respectively, at the time of PGF2α injection while FSH-10 group received 10 IU of FSH with sponge removal. In these four groups, estrus was detected for 5 days following sponge removal. Flushing+ram effect (F+RE) group ewes received dietary supplements for 28 days and ram effect for 14 days before the start of the mating period and were monitored for estrus for 4 weeks later. Control ewes monitored for estrus for two months without any treatment. Results. Birth rate was higher in F+RE group than eCG-500 and FSH-10 groups (p<0.05). Litter size was higher in eCG-700 group than F+RE and control groups (p<0.05). Fecundity was higher in eCG-700 group than FSH-10 group (p<0.05). Conclusions. 300 IU eCG at the end of progesterone application was sufficient to induce multiple lambing during the breeding season. Moreover, to increase lamb production should be taken necessary management measures for improve survival to weaning of multiple-born lambs.
Promising Mesenchymal Stem Cell Intervention for Relieving Cardiac Recovery against Cardiotoxic Injury Modeling with Doxorubicin: A Novel Therapeutic Approach
(Sciendo, 2025) Boztok Özgermen, Deva Başak; Haydardedeoğlu, Ali Evren; Yavuz, Orhan
Doxorubicin (DOX), a commonly used anti-neoplastic agent, has been associated with significant cardiotoxic effects, which limit its clinical utility. Recent studies suggest that mesenchymal stem cells (MSCs) may offer therapeutic potential in mitigating DOX-induced cardiotoxicity through their regenerative properties. This study aimed to evaluate the cardioprotective effects of fetal kidney-derived mesenchymal stem cells (FKD-MSCs) in a DOX-induced cardiotoxicity rat model. Thirty male Sprague-Dawley rats were divided into three groups: control, sham, and treatment. DOX (10 mg/kg) was administered to the sham and treatment groups to induce cardiotoxicity. The treatment group received intraperitoneal FKD-MSCs (2 × 106) three times at weekly intervals post-DOX administration. Immunohistochemical analyses were conducted to assess cardiac recovery. The 5-bromo-2-deoxyuridine (BrdU) labeling technique was used to track FKD-MSC localization in the cardiac tissue. The immunohistochemical findings demonstrated a significant improvement in the treatment group compared to the sham group. The BrdU-labeled FKD-MSCs were predominantly localized in cardiac muscle tissues, indicating their successful homing and integration into damaged cardiac regions. The results of the study indicate that FKD-MSCs significantly attenuated DOX-induced cardiotoxicity in rats, suggesting their potential as a novel therapeutic approach for cardioprotection. Further studies are warranted to investigate their clinical applications in managing chemotherapy-induced cardiotoxicity.
Keratoconjunctivitis sicca in intact Aksaray Malakli breed dogs: Evaluation of 50 cases
(John Wiley and Sons Inc, 2024) Avcı, Nihan; Gümüş, Fehmiye; Boztok Özgermen, Başak; Özen, Doğukan
Keratoconjunctivitis sicca (KCS) is characterized by decreased aqueous tear film components, leading to ocular surface damage. Diagnosis involves clinical examination, Schirmer Tear Test (STT1), and Tear Film Break-Up Time (TFBUT) measurement. This retrospective study aimed to assess dry eye syndrome in intact, purebred, Aksaray Malakli breed dogs, and investigate potential associations with hereditary ocular diseases due to inbreeding for phenotype preservation. Animals: Fifty healthy, purebred, intact Aksaray Malakli dogs of varying ages and sexes, all exhibiting conjunctival hyperemia and mucoid ocular discharge. Dogs with chronic diseases, systemic medication, or ongoing ophthalmic treatment were excluded. Procedures: Demographic data, ophthalmic examination results, and ocular anomalies were recorded. STT1 and TFBUT assessments were performed to measure tear production and film stability. STT1 values were scored from 1 (normal) to 4 (severe dry eye), and TFBUT ≥20 s was considered normal. Statistical analyses were used to evaluate correlations and differences. Results: The correlation between age and STT1 values (r = −.182, p =.206) was not statistically significant. Mean STT1 was higher in females than males, though not significantly (p =.849). Dogs without third eyelid gland prolapse had significantly higher STT1 and TFBUT measurements compared to those with prolapse (p =.027). No significant sex difference was found in the frequency of third eyelid prolapse (p =.289). A significant positive correlation was observed between STT1 and TFBUT (r =.924; p <.001). Conclusions: Aksaray Malakli dogs, particularly those with third eyelid gland prolapse, are predisposed to mild to moderate KCS. These findings suggest the need for further clinical and genetic investigations to better understand and manage dry eye syndrome in this breed.
Relationship between blood antioxidant paraoxo-nase-1 and the offspring yield in hair goats
(Trakia University, 2024) Dursun, Şükrü
Small ruminants bring unproductive pastures to the economy with products such as meat and milk. Although the management of Hair goats is difficult compared to that of sheep, they make better use of unproductive pastures than sheep. The birth rate of Hair goats is high, but the multiple birth rate is quite low. This study aimed to determine the relationship between the antioxidant paraoxonase-1 (PON-1), and the offspring yield in Hair goats. In the second week of September, 100 females were randomly selected and blood samples were taken 15 days before the introduction of male goats to the herd from both males and females. Blood was taken from the same animals again before starting milk-ing in May. Paraoxonase-1 values were found to be low and significantly different in both sexes in October compared to May. There was a statistical difference (P?0.05) in PON-1 level in terms of gender and birth type. The PON-1 level of Hair goats that gave birth to twins was like that of male goats and goats with single birth, while it was insignificant between single-birth and infertile goats. It was found that a low PON-1 value was associated with low fertility whereas twin births were closely related to high value of PON-1. PON-1 was found to be important for multiple births (P?0.05). As a result, it was concluded that the PON-1 level increased the birth rate and offspring yield in Hair goats.

Güncel Gönderiler