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Öğe Evaluation of thiol/disulphide and oxidant–antioxidant statusof dairy cows in periparturient and post-partum period(John Wiley and Sons Inc, 2024) Balkan, Burcu Menekşe; Meral, Öğünç; Çetintav, Bekir; Özkurt, Güzin; Sel, TevhideThiol/disulphide homeostasis (TDH) has a critical role in many cellular activities such as antioxidant protection. Alterations of oxidative stress in the transition period play an important role in development of some diseases and disorders in dairy cows. Objectives: The purpose of this study is to assess the total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), total thiol, native thiol, disulphide and lipid profile in Simmental and Montofon dairy cows (aged 2–3) before and after calving. Methods: Blood samples were collected 233–280 days of pregnancy and the 30 days of post-partum. Serum total thiol, native thiol and disulphide levels were determined as well as TAS, TOS and paraoxonase-1 (PON-1) levels were measured using colorimetric assays. Triglycerides (TG), total cholesterol, HDL cholesterol and LDL cholesterol levels were measured with an automatic analyser. Results: Total thiol (p = 0.038) and disulphide (p = 0.015) levels were higher after calving compare to pregnancy in Montofon. TAS was found lower (p < 0.001), and OSI was higher in both breeds (Montofon p = 0.012, Simmental p = 0.028) after calving than in pregnancy. When compared between pregnancy and after calving levels in the same breed, HDL was found to be higher after calving (p < 0.001) and TG was lower after calving (p = 0.020) in Montofon. PON (p = 0.090), HDL (p < 0.001) and cholesterol levels were found higher (p < 0.001) and TG level was lower (p < 0.001) after calving in Simmental. Conclusions: According to our results, we observed different responses between two breeds before and after calving. There are few studies about TDH in animal research, and this is the first study in the literature that evaluates the TDH along with oxidative stress and lipid profiles in dairy cows in the periparturient and post-partum perioÖğe Cryopreservation of ram semen: baicalein efficiency on oxidative stress, chromatin integrity, viability and motility post thaw(Frontiers Media SA, 2024) Avdatek, Fatih; Güngör, Şükrü; Gülhan, Mehmet Fuat; İnanç, Muhammed Enes; Olğaç, Kemal Tuna; Denk, Barış; Yeni, Deniz; Taşdemir, UmutBaicalein (B) has potential antioxidant properties, but it has not been tested as a ram semen extender. This study aimed to assess the impact of B on various sperm parameters and determine its potential influence on semen quality after the freeze-thawing process. During the breeding season, ejaculates were obtained from four rams with the aid of an artificial vagina. The collected mixed semen samples were divided into four groups: control (C; 0), B0.5 (0.5 mM), B1 (1 mM), and B2 (2 mM). After semen extension, the samples were loaded into 0.25 mL straws and stored for 2 h at 4°C prior to freezing in liquid nitrogen vapor and thawed in a water bath at 37°C. Among the groups, B0.5 demonstrated the highest progressive motility results, while B1 and B2 exhibited reduced motility (p < 0.05). In terms of high mitochondrial membrane potential, plasma membrane and acrosome integrity, and viability, B0.5 showed significantly superior outcomes to the other B groups (p < 0.05), although it was not significantly better than C. B1 displayed the highest plasma membrane integrity levels (p < 0.05). Notably, B2 displayed the lowest total antioxidant status levels among the groups (p < 0.05). The findings of this study suggested that the in vitro spermatological characteristics of ram spermatozoa such as progressive motility and chromatin integrity can be protected from the freeze-thawing process by using the 0.5 mM dose of baicalein as a semen extender. The treatment of sperm freezing might benefit from further in-depth research on the role of B in the improvement of cryoinjury and its underlying processes.Öğe Evaluation of the in vitro cryopreservative performance of Juniper berry oil (Juniperus communis) on frozen-thawed bull semen(Acta Veterinaria Brno, 2023) Güngör, Şükrü; Yeni, Deniz; İnanç, Muhammed Enes; Türkmen, Ruhi; Tuncer, Pürhan Barbaros; Gülhan, Mehmet Fuat; Avdatek, Fatih; Taşdemir, UmutThis study aimed to reveal the effects of juniper (Juniperus communis) berry oil (JB) when added to the Tris extender, on bull (Holstein) sperm motility characteristics, abnormal sperm ratio, DNA fragmentation and some oxidative stress markers. The pooled ejaculate was divided into five equal parts as control (C) and Tris diluent containing 25, 50, 100, 200 ?g/ml JB. Diluted semen was incubated at 4 °C for 4 h and then frozen in liquid nitrogen (?196 °C). After freezing-thawing, motility characteristics were determined using a sperm analyzer system. The results showed that the 25 ?g/ml dose of JB had an improving effect on overall sperm motility (P < 0.05). The total abnormal spermatozoa ratio was not significantly different between the groups (P > 0.05). The JB25 and JB50 groups had higher DNA integrity than the other groups (P < 0.05). This finding was supported by low malondialdehyde levels (P < 0.05). Especially the 25 ?g/ml dose of JB was found to have a remedial effect on DNA integrity, as well as increasing progressive and total motility rates. This study has revealed new information on the effects of JB on bull semen cryopreservation. Juniper berry oil may be used as an antioxidant in bull semen freezing.Öğe Investigation of ischemia-modified albumin and thiol/disulfide homeostasis for the determination of oxidative stress in sheep with toxoplasmosis(Elsevier B.V., 2023) Aydın, Ömer; Özkurt, Güzin; Çamkerken, İlker; Eren, Emre; Yanar, Kerim Emre; Aktaş, Mustafa SinanIn this study, the aim was to investigate the thiol/disulfide homeostasis (TDH) parameters and ischemia-modified albumin (IMA) (ABSU) levels to determine oxidative stress in sheep with toxoplasmosis. Twenty-four Morkaraman sheep used in this study were allocated into two groups, one control group and one experimental group. Each group included 12 sheep. Blood samples were taken from all sheep only once and blood serum levels of albumin (ALB) (g/L), IMA (ABSU), native thiol (NT) (?mol/L), total thiol (TT) (?mol/L), disulfide (Ds) (?mol/L), Ds/NT, Ds/TT, NT/TT were investigated. Our results showed that the IMA (ABSU) levels of toxoplasma group were significantly higher than control group (p < 0.05). However, NT (?mol/L) and TT (?mol/L) level of toxoplasma group were significantly lower than the control group (p < 0.05). While the oxidant and antioxidant parameters were positively correlated within themselves, a negative correlation was determined between the antioxidant and oxidant parameters. In the Receiver Operating Characteristic (ROC) analysis, the area under the ROC curve (AUC), sensitivity, cut off and specificity values were determined for IMA (ABSU) values. These values were determined as 0.812, 1.24, 91.7% and 75.0%, respectively. For TT (?mol/L), these values were obtained as 0.750, 330.55, 75.0% and 66.7%. It was concluded that oxidative stress is intensely formed in sheep with toxoplasmosis, thiol-disulphide homestasis markers, and IMA (ABSU) are valuable markers in determining oxidative stress in toxoplasmosis.Öğe Casirivimab/Imdevimab: Possible Candidate for COVID-19 Treatment in Pets: Traditional Review(Türkiye Klinikleri Yayınevi, 2023) Ender, Fatma; Sayıner, Serkan; Gençosman, Sevgi; Ceylanlı, Deniz; Özkurt, Güzin; Abacıoğlu, Nurettin; Özer Şehirli, AhmetThe coronavirus disease-2019 (COVID-19), over the last 3 years has globally resulted in catastrophic widespread losses. Prevention and control of this disease has become a global priority for researchers and medical professionals due to high mortality rates, treatment costs and various losses (economic and fatalities). In recent times, the fact that cats and dogs living in households which are being affected by the disease has caused panic among pet owners and animal lovers. Studies have shown that cats, especially young cats, are the group of animals which are most affected. Therefore, the treatment of COVID-19 in animals is an important topic for veterinary medicine. Monoclonal antibodies have also been considered a possible treatment modality due to the use of various agents to treat COVID-19 and the lack of clear demonstration of their efficacy. Casirivimab/imdevimab (CAS/IMD) is a monoclonal antibody cocktail approved for treating COVID-19. There was a high expectation that CAS/IMD combination directly affects the angiotensin converting enzyme-2 receptors, which plays an important role in the pathogenesis of the virus. Experimental and clinical studies have shown that CAS/IMD combination positively affects the prognosis of patients suffering from COVID-19, speeds up the recovery process and increases survival. We suggest that CAS/IMD can be considered protective in COVID-19 positive dogs and cats. The aim of this article is to review the studies related to the beneficial effects of CAS/IMD and its possible effectiveness in animals with COVID-19 to promote their research in future studies.Öğe The effect of supplementation of follicle-stimulating hormone (FSH) into the Ovsynch protocol to increase the pregnancy rate in cyclic lactating dairy cows(Veterinární a farmaceutická univerzita Brno, 2022) Yılmazbaş Mecitoğlu, Gülnaz; Keskin, Abdulkadir; Karakaya Bilen, Ebru; Taşdemir, UmutThe aim of this study was to determine the effects of the follicle stimulating hormone (FSH) supplemented in the Ovsynch protocol on pregnancy per timed artificial insemination (P/TAI) in cyclic dairy cows. All cows (n = 383) included in the study received the Ovsynch protocol. The cows in FSH3 (n = 92), FSH4 (n = 88), and FSH3&4 (n = 91) were administered 20 mg FSH on day 3, or day 4, or both on days 3 and 4 of the protocol, respectively, whereas the control group (n = 112) did not receive any FSH treatment. The mean follicle number at TAI and ovulation number after TAI were similar among the FSH groups. However, the mean follicular size was smaller (similar to 14.9 mm, P = 0.02) in FSH4 compared to the other groups (similar to 15.9 mm). Pregnancy/TAI of the FSH4 group on day 31 (59.1%) and day 62 (53.4%) was higher than those of other groups on day 31 (45.5% in control, 45.7% in FSH3, and 46.2% in FSH3&4) and day 62 (42.9% in control, 43.5% in FSH3, and 40.7% in FSH3&4), but the difference was non-significant. Likewise, FSH4 (62.7%) had more (P = 0.05) P/TAI than the other groups (48.6% in control, 47.7% in FSH3, and 49.4% in FSH3&4) in synchronized cows. The cows which responded to the first gonadotropin-releasing hormone (GnRH) of Ovsynch in the FSH4 group (64.8%) had higher (P < 0.02) P/AI rate than the responding control cows (44.6%). We concluded that application of FSH on day 4 in cyclic cows which were responsive to the Ovsynch protocol can yield higher pregnancy rates, but more studies are needed to better demonstrate this potential effect.Öğe Use of infrared thermography during ejaculation process and Its link with semen quality and freezability in dogs(MDPI, 2021) Tekin, Koray; İnanç, Muhammed Enes; Özen, Doğukan; Çil, Beste; Olğaç, Kemal Tuna; Yılmaz, Burak; Taşdemir, Umut; Tuncer, Pürhan Barbaros; Büyükleblebici, Serhat; Daşkın, Ali; Uysal, Ongun; Stelletta, CalogeroThis study aimed to describe the thermal variation of external reproductive tracts during ejaculation in relation to sperm quality in dogs. Forty-six adult fertile dogs were monitored using a thermal camera before, during and after the semen collection, taking into account penile and scrotal temperatures as reproductive thermal patterns while eye and perianal temperatures were recorded as complementary thermal patterns of behavioral response. The parameters were classified depending on age (?4 years and >4 years), body weight (BW) (?75 kg and >75 kg), sperm concentration (CON) (?300 million and >300 million), total testicular volume (TTV) (?600 cm3 and >600 cm3) and total ejaculation time (TET) (?800 s and >800 s) of the animals from which semen was collected successfully. Heavier males (p < 0.05) that have more consistent testicles (p < 0.01) as well as quicker ejaculate responders (p < 0.001) and lower scrotal temperature had better semen (? motility) freezability. The lower eye temperature prior to the ejaculation (p < 0.01), lower scrotal temperature following ejaculation (p < 0.01), and conversely, higher penile temperature during the ejaculation (p < 0.001) had a higher sperm concentration. Furthermore, the sperm freezability was negatively correlated with total ejaculation time (r = -0.39, p < 0.05) and sperm abnormalities were lower in the ejaculate of dogs having a higher temperature of the scrotum, bulbus and penis. In conclusion, infrared monitoring throughout semen collection in dogs can provide information on behavioral reactions during human manipulation, as well as semen quality and testicular functionality.Öğe The effct of hot-iron disbudding on Thiol-Disulphide Homeostasis in calves(Kafkas Üniversitesi, 2019) Erdoğan, Hasan; Çamkerten, İlker; Çamkerten, Güzin; Ural, Kerem; Erdoğan, Songül; Günal, İsmail; Erel, ÖzcanWe aimed to examine the effct of hot-iron disbudding on serum thiol-disulphide homeostasis levels as a marker of oxidant stress in relationship with trauma in calves under sedation, local anaesthesia, and the non-steroidal anti-inflmmatory drug ketoprofen. A total of 30 Holstein calves were enrolled in the study and allocated into three groups: disbudded following sedation with xylazine (n=10) (group I); disbudded following sedation (xylazine) and local anaesthesia with lidocaine (n=10) (group II); and disbudded after sedation (xylazine), local anaesthesia (lidocaine), and ketoprofen (n=10) (group III). Blood samples were withdrawn before (0. min) and 30, 60, 90, and 120 min after dehorning. Serum native thiols, total thiols, and disulphide levels were detected with a novel assay. Native thiol and total thiol levels were reduced in all groups without any significance during the study period. At the 90th min of the study, native thiol levels in group II were significantly lower than in groups I and III. There were no significant alterations in total thiol levels in both groups. Disulphide levels showed no significant changes in group, time, and group by time interactions, but at the 60th min, groups I and III had the lowest levels. Disulphite/ native thiol, disulphite/total thiol, and native thiol/total thiol levels had significant group alterations in the 60th min. The reduction of native thiol and total thiol levels in all groups without significance might be related to the antioxidant activity of plasma; however, it is thought that the pain management procedures should be related to the sensitive oxidative balance by thiols.Öğe Levels of serum 25 (OH) D3 in naturally infected lambs with giardia duodenalis(Afyon Kocatepe Üniversitesi, 2019) Çamkerten, Güzin; Erdoğan, Hasan; Alıç Ural, Ali; Çamkerten, İlker; Erdoğan, Songül; Ural, KeremGiardia duodenalis is a binuclear flagellated protozoan that can infect birds, reptiles and many mammals. Thepathogen is one of the most important causes of diarrhea in humans and farm animals. The aim of this study wasto determine the changes in serum 25-hydroxyvitamin D3 levels in naturally infected lambs with Giardia duodenalis.The animal material of the study was used Sakız sheep which had naturally infected with Giardia duodenalisbetween 15-35 days of age from the Aydın region (n = 30), healthy lambs in the same age range (n = 15) and bothsexes. Sterile stool samples with the method of rectal touching of fecal samples from infected and healthyanimals, blood samples were obtained in a total of 5 ml of v.jugularis according to the technique in order todetermine serum 25-hydroxyvitamin D3 levels. Giardia screening was performed by direct microscopicexamination of stool samples. 25-OH-D levels were measured with Savant brand fluorescent immunoassay devicefrom the obtained serum samples. Mann-Whitney U test was used in statistical analysis. Statistically significantchanges were observed in 25-hydroxyvitamin D3 levels of healthy and natural infected animals (p <0.05).Öğe The effect of gallic acid addition to tris-based extender on frozen bull semen(Kafkas Üniversitesi, 2021) Güngör, Şükrü; İnanç, Muhammed Enes; Avdatek, Fatih; Türkmen, Ruhi; Taşdemir, UmutThis study aimed to determine the probable protective effect of gallic acid (GA) on the spermatological parameters of frozen-thawed bull semen. Ejaculates were collected from four Holstein bulls. The mixed ejaculate was divided into four equal groups as control (0 pg/mL GA), 50, 100 and 200 mu g/mL GA with a Tris-based extender. All extended groups were cooled, equilibrated into a +4 degrees C cold cabinet and loaded into straws. The straws were then frozen and stored In a liquid nitrogen container (-196 degrees C). Subsequently, samples were thawed in a water bath for analyzing motility and kinematic parameters, morphological integrity, DNA damage and biochemical alterations. GA50 (28.76 +/- 0.51%) and GA100 (29.32 +/- 0.31%) had improved progressive motility in comparison to the other groups (P<0.05). The highest total motility (69.71 +/- 0.52%) was detected in GA100 (P<0.05). Besides, the lowest DNA damage and total abnormality values were detected in the GA100 group (P<0.05). GA100 had the highest total antioxidant capacity, as well as the lowest malondialdehyde (MDA) level (P<0.05). In conclusion, GA 50 and 100 mu g/mL protects the progressive motility, morphological and DNA integrity by improving the total antioxidant status from the harmful effects of the freezing and thawing protocol.Öğe Efficiency of two different synchronization protocols in conception in simmental heifers(Afyon Kocatepe Üniversitesi, 2020) Bulut, Gaye; Taşdemir, UmutThis study aimed to reveal the effects of timed artificial insemination applied after the synchronization protocol known as day5 (D5) and the prostaglandin F2? (PGF2?) protocol applied in 11-day intervals (D11) on pregnancy rates in Simmental heifers. A total of 88 heifers were divided into two treatment groups. In the D5 group (n: 56), after gonadotrophin releasing hormone (GnRH) application, PGF2? injection was made on the 5th day and the second PGF2? injection was given one day later. The second GnRH was applied at the time of TAI and at the 44th hour of the second PGF2? application. In the D11 group (n: 32), double-dose PGF2? was given in 11-day intervals. GnRH was applied at the time of TAI and at the 81st hour following the second PGF2? injection. Pregnancy-associated glycoprotein visual test was carried out for determining to pregnancy on the 28th day. Additionally, for the purpose of determining embryonic deaths, the pregnancies were checked again by ultrasonography on the 63rd day following TAI. The 28th-day pregnancy rates in the synchronized heifers were 51.8% (29/56) in the D5 group and 43.8% (14/32) in the D11 group P>0.05. The 63rd-day pregnancy rates were 48.21% (27/56) in the D5 group and 40.62% (13/32) in the D11 group P>0.05. Consequently, although there was no statistically significant difference in terms of pregnancy rates between the D5 and D11 protocols, there was a numerical increase in the D5 protocol. Considering its short application time, D5 may be used for TAI in Simmental heifers.Öğe An investigation of thiol/disulfide homeostasis and ischemia-modified albumin levels to assess the oxidative stress in dogs with canine distemper(Zagrab University, 2021) Değirmençay, Şükrü; Camkerten, Güzin; Camkerten, İlker; Aktaş, M. SinanThis study aimed to investigate the diagnostic value of thiol/disulfide homeostasis (TDH) and ischemia-modified albumin (IMA) levels in evaluating oxidative stress in dogs with canine distemper. The study materials consisted of 24 cross-breed dogs: 18 dogs with canine distemper (CD group) and 6 healthy dogs as the control. Blood samples for analysis of antioxidant-oxidant parameters were taken from both groups. In addition to TDH parameters (native thiol [NT], total thiol [TT], and disulfide [Ds] levels and Ds/NT, Ds/TT, and NT/TT ratios), IMA and albumin (ALB) levels were also determined in the blood serum of the groups. The IMA levels of the CD group were significantly higher than the control group (P<0.05). Antioxidant parameters (NT, TT) and oxidant parameters (Ds, Ds/NT, and Ds/TT) both exhibited a significant positive correlation among themselves (P<0.01). The areas under the ROC curve of IMA, Ds/NT, and Ds/TT were 0.78, 0.62, and 0.62 respectively. The high levels of IMA, Ds, Ds/NT, and Ds/TT and low levels of NT, TT, NT/TT and ALB in dogs with canine distemper can be used to assess the oxidative stress caused by the disease. Overall, the diagnostic performances of IMA. Ds/NT, and Ds/TT for detection of increased oxidative stress were similar, but IMA was superior.Öğe Red pine (Pinus brutia Ten) bark tree extract preserves sperm quality by reducing oxidative stress and preventing chromatin damage(Blackwell Publishing Ltd, 2020) Taşdemir, Umut; Yeni, Deniz; İnanç, Muhammed Enes; Avdatek, Fatih; Çil, Beste; Türkmen, RuhiThis study aimed to investigate the effectiveness of using red pine bark tree extract (P; Pinus brutia Ten) as a TRIS extender in an attempt to prevent oxidative stress in bull spermatozoa after freezing. Semen specimens were obtained from Simmental bulls via an artificial vagina and pooled. They were separated into five specimens and diluted with Tris extender consisting of P (200, 100, 50 and 25 µg/ml) and P free (control; C) up to a final concentration of 16 × 106 per straw. All specimens were equilibrated for a period of 4 hr at a temperature of 4°C, following which they were filled in 0.25-ml French straws and frozen. Addition of P resulted in favourable tail length in comparison with C (p <.05). The lowest malondialdehyde levels and the highest glutathione levels were detected in all P groups (p <.05). Supplementation with P did not show advanced results in terms of total, progressive sperm motility and total abnormality in comparison with C (p >.05). In conclusion, it has been shown that although P added to a Tris extender does not have a positive effect on sperm motility, it prevents chromatin damage by reducing oxidative stress, in addition to reducing head abnormalities when used at the amount of 50 ?g/ml.Öğe Serum thiol disulphide levels among sheep with sarcoptic mange(Veteriner Fakultesi Dergisi, 2019) Çamkerten, İlker; Çamkerten, Güzin; Erdo?an, Hasan; Ayan, Adnan; Erdoğan, Songül; Ural, KeremSarcoptic mange, a notable parasitic disease, causes dermatological alterations among ruminants. Thiol-disulphite hemostasis is a novel oxidative stress parameter. The aim of this study was to evaluate dynamic thiol/disulfide homeostasis in sheep with sarcoptic mange. Total of thirty-six sheep (n=15 female, n=21 male) with sarcoptic mange (Group I), and twelve healthy sheep (Group II) were used in the study. A novel method was used to determine the thiol disulphide parameters. Native thiol, total thiol and Disulphide values were statistically lower in Group I. Disulphide/native thiol, Disulphide/total thiol, and Native thiol/ total thiol proportions had no statistical differences in groups. Sarcoptic mange was probably affected by the thiol Disulphide hemostasis in infected sheep. Thus, the data obtained in this study might form base for further studies to include antioxidant molecules in the treatment protocols.Öğe Rasyon çinko miktarının boğalarda sperma miktar ve kalitesine etkisi(Türkiye Cumhuriyeti Tarım ve Orman Bakanlığı, 2014) Ünay, Engin; Yaman, Sema; Kinet, Hüseyin; Tuncer, Pürhan Barbaros; Büyükleblebici, Serhat; Karakaş, VedatAraştırmada, hayvan materyali olarak, Hayvancılık Merkez Araştırma Enstitüsünde, dondurulmuş sperma üretimi yapılan 9 baş Siyah Alaca ırkı boğa kullanılmıştır. Araştırma, karma yemde 40 mg/kg çinko içeren kontrol grubu ve kontrol grubunda kullanan karma yeme ilave olarak 110mg/kg çinko (toplam 150 mg/kg Zn içeren 1. grup) ve 160 mg/kg (toplam 200 mg/kg Zn içeren 2. grup) çinko eklenen 3 farklı muamele grubundan oluşmuştur. Her gruba tesadüfi olarak 3er baş boğa dağıtılmıştır. Çinko preparatı olarak, kontrol grubu ve diğer iki muamele grubunda organik çinko bileşiği Bioplex Zinc (Aminoasit hidratin çinko şelat, Alltech) kullanılmıştır. Deneme başlangıcında, 60 gün ön yemleme yapılmıştır. Bu süre sonunda boğalardan suni vajen yöntemiyle 5 hafta boyunca, haftada 2 defa 2’şer ejakülat sperma alınmıştır. Sulandırılan spermalar, ekilibrasyondan sonra 0.25 ml’lik payetlere çekilerek, kontrollü dondurma cihazında dondurulup sıvı azot içinde saklanmıştır. Nativ spermatolojik değerlendirmeler aynı gün, çözüm sonu değerlendirmeleri ise 24 saatlik dondurma sürecinden sonra payetler 37°C’de 30 saniye süreyle çözdürülerek değerlendirilmiştir. Çalışmada sperma miktarı yönünden kontrol grubu ile 200 mg/kg çinko içeren 2. grup arasından fark önemsiz, kontrol grubu ile 150 mg/kg çinko içeren 1. grup arasındaki fark önemli (P<0,05) bulunmuştur. Motilite oranı, mass aktivite değeri ve yoğunluk yönünden kontrol grubu ile 1. ve 2. gruplar arasındaki fark önemli bulunmuştur (P<0,05). Sperma yoğunluğu bakımından en iyi sonuç 1. gruptan alınmıştır. Çözüm sonu en iyi motilite değeri ise 2. grupta elde edilmiştir. Damızlık boğaların beslenmesinde, karma yemde 40 mg/kg çinko (NRC 2001) kullanılması yerine, 150 veya 200 mg/ kg çinko kullanılmasının sperma miktarı ve kalitesi üzerine olumlu etki yaptığı kanaatine varılmıştır.Öğe Effect of ?--Lipoic acid supplementation to extender on quality of frozen-thawed bull semen(Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 2015) Büyükleblebici, Olga; Büyükleblebici, Serhat; Tuncer, Pürhan Barbaros; Özgürtaş, Taner; Taşdemir, UmutThis study was conducted to investigate the possible protective effect of ?--lipoic acid (ALA) supplementation to extender on damages in the quality of bull semen elicited by freze-thawing process-induced oxidative stres. Ejaculates were collected via an artificial vagina from the bulls at once a week. Then they were split into five aliquots and extended with the Tris base extender containing different doses of ALA, except control. The extended samples were equilibrated slowly to 4°C for 4 h and then frozen using a digital freezing machine. Frozen straws were thawed to analyse progressive motility and sperm motility characteristics as well as plasma membrane integrity. Biochemical assays were performed in a spectrophotometer using commercial kits. Treatment of extender with ALA groups caused a significant decrease in percentages of post-thaw sperm CASA, progressive motilities and sperm motility characteristics such as VAP, VSL and VCL except ALA0.5. Besides ALA0.5 showed lower percentages of acrosome and total abnormalities in comparison to the control. In conclusion, findings generated here showed that ALA0.5 supplementation in Tris based semen extender was of great beneficial effect on frozen-thawed bull semen in terms of morphology and plasma membrane integrityÖğe The effects of different antioxidants on post-thaw microscopic and oxidative stress parameters in the cryopreservation of Brown-Swiss Bull Semen(Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 2016) Büyükleblebici, Olga; Büyükleblebici, Serhat; Taşdemir, Umut; Tuncer, Pürhan BarbarosThe aim of this study was to research the effects of four antioxidants that had been added to semen diluents, on spermatological parameters, anti-oxidant enzymes activities and DNA integrity after the freeze-thaw procedures in Brown-Swiss bull semen. A total of 24 ejaculates were collected from three bulls. Each ejaculate was divided into five equal parts and they diluted to the Tris + 25 mM trehalose base extender containing fetuin 1 mg/ml, dithioerithritol 1 mM, cysteamine 4 mM or linoleic acid 0.5 ml/50 ml, and no additives (control). Group dithioerithritol and linoleic acid showed the higher rates of CASA progressive motility, CASA sperm motility (P<0.05) compared with the other groups. Group linoleic acid provided the better protective effect for acrosome and total abnormalities (P<0.01, P<0.05; respectively). Also, group dithioerithritol showed the highest values for the HOS test (P<0.01). In the comet test, group dithioerithritol enhanced tail with lesser chromatin damage than the other groups (P<0.05). Supplementation of dithioerithritol significantly affected the GSH activity (P<0.01). Also groups dithioerithritol and linoleic acid gave higher CAT values than the other groups (P<0.05). Additionally, supplementation of fetuin and cysteamine showed the lowest total antioxidant activity value (P<0.05). In conclusion, we may said that, the addition of antioxidants, specially dithioerithritol and linoleic acid, to added with 25 mM trehalose Tris extender improve post-thaw sperm parameters.Öğe Effects of various cryoprotectants on bull sperm quality, DNA integrity and oxidative stress parameters(Academic Press, 2013) Taşdemir, Umut; Büyükleblebici, Serhat; Tuncer, Pürhan Barbaros; Coşkun, Erdem; Özgürtaş, Taner; Aydın, Fevzi Nuri; Büyükleblebici, Olga; Gürcan, İsmail SafaThe objectives of this study was to compare the effects of type and concentration of cryoprotectants glycerol (G), ethylene glycol (EG) and dimethyl sulfoxide (DMSO) on the plasma membrane and DNA integrity as well as antioxidant activity of cryopreserved Eastern Anatolian red bull sperm. Ejaculates were collected from the three bulls using an artificial vagina twice a week. The ejaculates were pooled to increase the semen volume for replication and to eliminate variability among the evaluated samples. The pooled ejaculates were also split into seven equal experimental groups and diluted with the modified base extender to a final spermatozoa concentration of 15 x 10(6) /ml. The extended samples were cooled slowly to 4 degrees C and equilibrated for 4 h. They were then loaded into 0.25 ml French straws and frozen using a digital freezing machine at 3 programmed rates: -3 degrees C/min from +4 degrees C to -10 degrees C, -40 degrees C/min from -10 degrees C to -100 degrees C, and -20 degrees C/min from -100 degrees C to -140 degrees C. Thereafter, the straws were plunged into liquid nitrogen at -196 degrees C. Frozen straws were thawed individually at 37 degrees C for 30 s in a water bath to analyse progressive motility and sperm motion characteristics as well as membrane integrity using hypo-osmotic swelling test. Biochemical assays were performed in a spectrophotometer using commercial kits. DNA damage was evaluated by Comet Assay using Image Analysis System. 6% G exhibited the greatest percentages of CASA (43.7 +/- 2.92%) and progressive (26.4 +/- 2.64%) motilities when compared to the other groups (P < 0.001). 6% G and 6% EG showed the greatest values of preserved membrane integrity (P < 0.001). 6% DMSO and 3% EG + 3% DMSO resulted in greater chromatin damage than the other groups (P < 0.001). The antioxidant activities of GPx, GSH, and CAT as well as the total antioxidant activity were affected by the type of cryoprotectant; notably, 2% G + 2% EG + 2% DMSO yielded the lowest activities when compared to the other groups (P < 0.001). In conclusion, no advantages were found in using EG or DMSO to replace G in bull sperm cryopreservation. Freezing with cryoprotectant 6% G yielded the best post-thaw sperm characteristics for Eastern Anatolian Red bull spermatozoa. (c) 2012 Elsevier Inc. All rights reserved.Öğe Cryopreservation of rainbow trout oncorhynchus mykiss spermatozoa: effects of extender supplemented with different antioxidants on sperm motility, velocity and fertility(Academic Press, 2014) Kutluyer, Filiz; Kayim, Murathan; Ogretmen, Fatih; Büyükleblebici, Serhat; Tuncer, Pürhan BarbarosIn present study, it was examined whether addition of different antioxidants to the cryopreservation extenders had an effect on semen post-thaw fertility and motility in rainbow trout (Oncorhynchus mykiss) and also it was investigated the sperm characteristics post-thaw sperm characteristics and fertility. The collected semen was pooled to minimize individual variation. Each pooled ejaculate was split into 12 equal aliquots and diluted with base extenders supplemented with the antioxidants, and a base extender with no additives (control). The pooled semen samples diluted at the ratio of 1:10 by the extenders were subjected to cryopreservation. Antioxidants were separately added to the extenders (one per experimental group): catalase (250 U/l), superoxide dismutase (250 U/l), peroxidase (250 U/l), oxidized glutathione (1.5 mmol/l), reduced glutathione (1.5 mmol/l), L-methionine (1.5 mmol/l), uric acid (0.25 mmol/l), L-ascorbic acid (0.5 mmol/l), alpha-tocopherol (2.0 mmol/l), beta-carotene (0.5 mmol/l) and camitine (0.5 mmol/l). After dilution the semen was aspirated into 0.25 ml straws, the straws were placed on the tray, frozen for 10 min, and plunged into liquid nitrogen. Our results indicated that the post-thaw motility rate increased in extenders supplemented with uric acid, L-methionine, SOD, L-carnitine, alpha-tocopherol and L-reduced glutathione (p < 0.05). The motility duration of frozen thawed semen increased in extenders supplemented with uric acid, L-methionine, SOD, alpha-tocopherol and L-reduced glutathione (p < 0.05). Fertilization rate and hatching rate of frozen-thawed semen was not affected by the tested antioxidants. Consequently, the tested antioxidants affected the motility parameters and cryopreservation extenders could be supplement with antioxidants. This study suggested usage of antioxidants in the cryopreservation of rainbow trout.Öğe Comparing ethylene glycol with glycerol and with or without dithiothreitol and sucrose for cryopreservation of bull semen in egg-yolk containing extenders(Academic Press, 2014) Büyükleblebici, Serhat; Tuncer, Pürhan Barbaros; Bucak, Mustafa Numan; Taşdemir, Umut; Eken, Ayşe; Büyükleblebici, Olga; Durmaz, Emre; Sarıözkan, Serpil; Endirlik, Burcu ÜnlüThere are few studies performed for investigating the roles of different ratio and cryoprotectants with dithiothreitol or sucrose on sperm motility characteristics and antioxidant capacities of post-thawed bull spermatozoa. The objectives of this study were to compare glycerol (G) and ethylene glycol (EG) at different concentrations as cryoprotectants and dithiothreitol (D) or sucrose (S) (with/without) as antioxidants in Tris extender for cryopreservation of bull semen. Twenty-four ejaculates obtained from three bulls were included in the study. Each ejaculate was split into four equal aliquots and diluted using both of the Tris extenders with glycerol (5% or 7%) or ethylene glycol (3% or 5%). After that, each extenders were split into three equal aliquots and diluted using both of the dithiothreitol 5 mM or sucrose 25 mM, and control (without additives) was cooled to 4 degrees C and frozen in 0.25-ml French straws. when compared to control, different doses cryoprotectants and antioxidants addition no significantly increased the percentages of post-thaw sperm progressive and motitilities, acrosome abnormality and plasma membrane integrity (P > 0.05). However, EG3 + S yielded the greatest percentages of the total abnormality (P < 0.05). As regard to antioxidant activities G7 and EG5 led to lowest MDA activity with or without D or S but, these results were not supported to the GPx activity (P < 0.01). The sperm motion characteristics such as VAP, VCL, ALH and BCF gave significantly different results (P < 0.05). When compared the DNA integrity, different doses cryoprotectants without antioxidants addition significantly increased the percentages the tail intensity and tail moment (P < 0.05). There were no significant differences observed in non-return rates among all treatment groups (P > 0.05). (C) 2014 Elsevier Inc. All rights reserved.
