The effect of gallic acid addition to tris-based extender on frozen bull semen
Yükleniyor...
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Kafkas Üniversitesi
Erişim Hakkı
Attribution-NonCommercial-NoDerivs 3.0 United States
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
Özet
This study aimed to determine the probable protective effect of gallic acid (GA) on the spermatological parameters of frozen-thawed bull semen. Ejaculates were collected from four Holstein bulls. The mixed ejaculate was divided into four equal groups as control (0 pg/mL GA), 50, 100 and 200 mu g/mL GA with a Tris-based extender. All extended groups were cooled, equilibrated into a +4 degrees C cold cabinet and loaded into straws. The straws were then frozen and stored In a liquid nitrogen container (-196 degrees C). Subsequently, samples were thawed in a water bath for analyzing motility and kinematic parameters, morphological integrity, DNA damage and biochemical alterations. GA50 (28.76 +/- 0.51%) and GA100 (29.32 +/- 0.31%) had improved progressive motility in comparison to the other groups (P<0.05). The highest total motility (69.71 +/- 0.52%) was detected in GA100 (P<0.05). Besides, the lowest DNA damage and total abnormality values were detected in the GA100 group (P<0.05). GA100 had the highest total antioxidant capacity, as well as the lowest malondialdehyde (MDA) level (P<0.05). In conclusion, GA 50 and 100 mu g/mL protects the progressive motility, morphological and DNA integrity by improving the total antioxidant status from the harmful effects of the freezing and thawing protocol.
Açıklama
Anahtar Kelimeler
Antioxidant, Bull Semen, DNA Damage, Gallic Acid
Kaynak
Kafkas Üniversitesi Veteriner Fakültesi Dergisi
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
27
Sayı
5
