The effect of gallic acid addition to tris-based extender on frozen bull semen
| dc.contributor.author | Güngör, Şükrü | |
| dc.contributor.author | İnanç, Muhammed Enes | |
| dc.contributor.author | Avdatek, Fatih | |
| dc.contributor.author | Türkmen, Ruhi | |
| dc.contributor.author | Taşdemir, Umut | |
| dc.date.accessioned | 2021-12-13T05:38:08Z | |
| dc.date.available | 2021-12-13T05:38:08Z | |
| dc.date.issued | 2021 | |
| dc.department | Teknik Bilimler Meslek Yüksekokulu | |
| dc.description.abstract | This study aimed to determine the probable protective effect of gallic acid (GA) on the spermatological parameters of frozen-thawed bull semen. Ejaculates were collected from four Holstein bulls. The mixed ejaculate was divided into four equal groups as control (0 pg/mL GA), 50, 100 and 200 mu g/mL GA with a Tris-based extender. All extended groups were cooled, equilibrated into a +4 degrees C cold cabinet and loaded into straws. The straws were then frozen and stored In a liquid nitrogen container (-196 degrees C). Subsequently, samples were thawed in a water bath for analyzing motility and kinematic parameters, morphological integrity, DNA damage and biochemical alterations. GA50 (28.76 +/- 0.51%) and GA100 (29.32 +/- 0.31%) had improved progressive motility in comparison to the other groups (P<0.05). The highest total motility (69.71 +/- 0.52%) was detected in GA100 (P<0.05). Besides, the lowest DNA damage and total abnormality values were detected in the GA100 group (P<0.05). GA100 had the highest total antioxidant capacity, as well as the lowest malondialdehyde (MDA) level (P<0.05). In conclusion, GA 50 and 100 mu g/mL protects the progressive motility, morphological and DNA integrity by improving the total antioxidant status from the harmful effects of the freezing and thawing protocol. | |
| dc.identifier.doi | 10.9775/kvfd.2021.26023 | |
| dc.identifier.endpage | 639 | en_US |
| dc.identifier.issue | 5 | en_US |
| dc.identifier.startpage | 633 | en_US |
| dc.identifier.uri | https:/dx.doi.org/10.9775/kvfd.2021.26023 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12451/8927 | |
| dc.identifier.volume | 27 | en_US |
| dc.identifier.wos | WOS:000704282200013 | |
| dc.identifier.wosquality | Q4 | |
| dc.indekslendigikaynak | Web of Science | |
| dc.language.iso | en | |
| dc.publisher | Kafkas Üniversitesi | |
| dc.relation.ispartof | Kafkas Üniversitesi Veteriner Fakültesi Dergisi | |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | |
| dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | * |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | * |
| dc.subject | Antioxidant | |
| dc.subject | Bull Semen | |
| dc.subject | DNA Damage | |
| dc.subject | Gallic Acid | |
| dc.title | The effect of gallic acid addition to tris-based extender on frozen bull semen | |
| dc.type | Article |
