Conjugation of polymer-coated gold nanoparticles with antibodies—synthesis and characterization

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dc.authoridBayram, Cem -- 0000-0001-8717-4668; Pelaz, Beatriz -- 0000-0002-4626-4576; biomaGUNE, CIC -- 0000-0001-7690-0660; Soliman, Mahmoud -- 0000-0003-4260-0808; ONUR, MEHMET -- 0000-0002-3630-7982; del Pino, Pablo -- 0000-0003-1318-6839; Tan, Gamze -- 0000-0002-2755-1756; Valdeperez, Daniel -- 0000-0002-2876-5545
dc.contributor.authorTan, Gamze
dc.contributor.authorKantner, Karsten
dc.contributor.authorZhang, Qian
dc.contributor.authorSoliman, Mahmoud G.
dc.contributor.authorDel Pino, Pablo
dc.contributor.authorParak, Wolfgang Johann
dc.contributor.authorOnur, Mehmet Ali
dc.contributor.authorValdeperez, Daniel
dc.contributor.authorRejman, Joanna
dc.contributor.authorPelaz, Beatriz
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:27:26Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:27:26Z
dc.date.issued2015
dc.departmentSabire Yazıcı Fen-Edebiyat Fakültesi
dc.description.abstractThe synthesis of polymer-coated gold nanoparticles with high colloidal stability is described, together with appropriate characterization techniques concerning the colloidal properties of the nanoparticles. Antibodies against vascular endothelial growth factor (VEGF) are conjugated to the surface of the nanoparticles. Antibody attachment is probed by different techniques, giving a guideline about the characterization of such conjugates. The effect of the nanoparticles on human adenocarcinoma alveolar basal epithelial cells (A549) and human umbilical vein endothelial cells (HUVECs) is probed in terms of internalization and viability assays.
dc.description.sponsorshipGerman Research Foundation [DFG GRK 1782]; European Commission (grant FutureNanoNeeds); Alexander von Humboldt Foundation; TUBITAK [2214A]; Chinese Scholarship Council (CSC); Youssef Jameel Foundation
dc.description.sponsorshipThis work was supported by the German Research Foundation (DFG GRK 1782 to Wolfgang J. Parak) and by the European Commission (grant FutureNanoNeeds to Wolfgang J. Parak). Beatriz Pelaz acknowledges a fellowship from the Alexander von Humboldt Foundation. Gamze Tan thanks TUBITAK for financial support through the 2214A-International Research Fellowship Programme. Qian Zhang is grateful to a Chinese Scholarship Council (CSC) for a fellowship. Mahmoud G. Soliman thanks the Youssef Jameel Foundation for a PhD fellowship.
dc.identifier.doi10.3390/nano5031297
dc.identifier.endpage1316en_US
dc.identifier.issn2079-4991
dc.identifier.issue3en_US
dc.identifier.pmid28347065
dc.identifier.scopusqualityQ1
dc.identifier.startpage1297en_US
dc.identifier.urihttps://doi.org/10.3390/nano5031297
dc.identifier.urihttps://hdl.handle.net/20.500.12451/5862
dc.identifier.volume5en_US
dc.identifier.wosWOS:000362643900011
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publishermdpi
dc.relation.ispartofNanomaterials
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectGold Nanoparticles
dc.subjectBioconjugation
dc.subjectNanoparticle Characterization
dc.subjectToxicity
dc.subjectNanoparticle-cell Interaction
dc.subjectCellular Uptake
dc.subjectVEGF
dc.titleConjugation of polymer-coated gold nanoparticles with antibodies—synthesis and characterization
dc.typeArticle

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