Conjugation of polymer-coated gold nanoparticles with antibodies—synthesis and characterization
Yükleniyor...
Dosyalar
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
mdpi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The synthesis of polymer-coated gold nanoparticles with high colloidal stability is described, together with appropriate characterization techniques concerning the colloidal properties of the nanoparticles. Antibodies against vascular endothelial growth factor (VEGF) are conjugated to the surface of the nanoparticles. Antibody attachment is probed by different techniques, giving a guideline about the characterization of such conjugates. The effect of the nanoparticles on human adenocarcinoma alveolar basal epithelial cells (A549) and human umbilical vein endothelial cells (HUVECs) is probed in terms of internalization and viability assays.
Açıklama
Anahtar Kelimeler
Gold Nanoparticles, Bioconjugation, Nanoparticle Characterization, Toxicity, Nanoparticle-cell Interaction, Cellular Uptake, VEGF
Kaynak
Nanomaterials
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
5
Sayı
3
