Co-evaluation of interaction parameters of genomic and plasmid DNA for a new chromatographic medium
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Date
2019
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier B.V.
Access Rights
info:eu-repo/semantics/embargoedAccess
Abstract
Preparation of new sorbents specific to DNA has a great significance in many biomedical fields. This study reports a new sorbent with high surface area and porosity to immobilize nucleic acids having both high molecular weight like genomic DNA (gDNA) for potential use in therapy of some immune system disease and low molecular weight like plasmid DNA (pDNA) for diagnosis, gene therapy and DNA vaccination. For this aim, silica-based pore-expanded SBA-15 nanoparticles with aminopropyl-trimethoxysilane (APTMS) for decoration of Fe+3 ions (PE SBA-15-APTMS/Fe+3) were synthesized to get high surface area for high adsorption, and embedded into cryogel column for obtaining interconnected pores to avoid diffusion limitation of DNA samples because of their viscosity features. SEM, XRD, BET, and FTIR techniques were used for characterization of samples. Synthesized hybrid column showed a superior adsorption capacity of 751.5 mg/g NP for gDNA at pH 6 with an initial concentration of 2.0 mg/mL. Hybrid column presented excellent performance for pDNA when evaluated with agarose gel electrophoresis.
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Description
*Önal, Burcu ( Aksaray, Yazar ) *Odabaşı, Mehmet ( Aksaray, Yazar )
Keywords
DNA Adsorption, Hybrid Cryogels, Pore Expanded SBA-15
Journal or Series
International Journal of Biological Macromolecules
WoS Q Value
N/A
Scopus Q Value
Q1
Volume
141
Issue
-
