Supplementation of quercetin for advanced DNA integrity in bull semen cryopreservation

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dc.authoridTURKMEN, Ruhi -- 0000-0003-4726-3900; Tasdemir, Umut -- 0000-0003-2827-1286; INANC, MUHAMMED ENES -- 0000-0001-6954-6309
dc.contributor.authorAvdatek, Fatih
dc.contributor.authorYeni, Deniz
dc.contributor.authorİnanç, Muhammed Enes
dc.contributor.authorÇil, Beste
dc.contributor.authorTuncer, B. P.
dc.contributor.authorTürkmen, Ruhi
dc.contributor.authorTaşdemir, Umut
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:14:51Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:14:51Z
dc.date.issued2018
dc.departmentAksaray Teknik Bilimler Meslek Yüksekokulu
dc.description.abstractThe aim of this study was to identify the effects of adding quercetin (Q) to Tris extender in order to identify levels of oxidative stress in bull sperm after freeze thawing. Ejaculates were collected via artificial vagina from Holstein bulls. Semen was divided into five tools and diluted to a final concentration of 15x10(6) spermatozoa/ml with the Tris extender containing Q (25, 50, 100 and 200g/ml) and no additive (control; C). All examples were equilibrated at 4 degrees C during 4hr then were loaded into 0.25-ml straws and frozen using a controlled rate. Sperm motility and motility characteristics were determined using the computer-assisted semen analyser. Sperm membrane integrity was assessed using the hypoosmotic swelling test. Sperm chromatin integrity was investigated using the single cell gel electrophoresis. Total antioxidant capacities were performed colorimetrically. Q supplementation used as an antioxidant did not produce better results in the proportion of sperm progressive and total motility, plasma membrane integrity and sperm abnormalities. Q supplementation exhibited the favourable tail length, tail DNA and tail moment. In conclusion, when whole parameters are considered, Q25 can be added to the Tris extender due to its positive effect on sperm DNA integrity and no adverse effect on the progressive and total motilities of sperm.
dc.description.sponsorshipGeneral Directorate of Agribusiness, Ankara, Turkey
dc.description.sponsorshipThis study was supported by General Directorate of Agribusiness, Ankara, Turkey.
dc.identifier.doi10.1111/and.12975
dc.identifier.issn0303-4569
dc.identifier.issn1439-0272
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1111/and.12975
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4179
dc.identifier.volume50en_US
dc.identifier.wosWOS:000429536900034
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofAndrologia
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectAntioxidant Activity
dc.subjectCryopreservation
dc.subjectDNA Integrity
dc.subjectOxidative Stress
dc.subjectQuercetin
dc.titleSupplementation of quercetin for advanced DNA integrity in bull semen cryopreservation
dc.typeArticle

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