Supplementation of quercetin for advanced DNA integrity in bull semen cryopreservation
Yükleniyor...
Dosyalar
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/embargoedAccess
Özet
The aim of this study was to identify the effects of adding quercetin (Q) to Tris extender in order to identify levels of oxidative stress in bull sperm after freeze thawing. Ejaculates were collected via artificial vagina from Holstein bulls. Semen was divided into five tools and diluted to a final concentration of 15x10(6) spermatozoa/ml with the Tris extender containing Q (25, 50, 100 and 200g/ml) and no additive (control; C). All examples were equilibrated at 4 degrees C during 4hr then were loaded into 0.25-ml straws and frozen using a controlled rate. Sperm motility and motility characteristics were determined using the computer-assisted semen analyser. Sperm membrane integrity was assessed using the hypoosmotic swelling test. Sperm chromatin integrity was investigated using the single cell gel electrophoresis. Total antioxidant capacities were performed colorimetrically. Q supplementation used as an antioxidant did not produce better results in the proportion of sperm progressive and total motility, plasma membrane integrity and sperm abnormalities. Q supplementation exhibited the favourable tail length, tail DNA and tail moment. In conclusion, when whole parameters are considered, Q25 can be added to the Tris extender due to its positive effect on sperm DNA integrity and no adverse effect on the progressive and total motilities of sperm.
Açıklama
Anahtar Kelimeler
Antioxidant Activity, Cryopreservation, DNA Integrity, Oxidative Stress, Quercetin
Kaynak
Andrologia
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
50
Sayı
4
