Cryopreservation of rainbow trout oncorhynchus mykiss spermatozoa: effects of extender supplemented with different antioxidants on sperm motility, velocity and fertility

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dc.authoridKUTLUYER, Filiz -- 0000-0001-8334-5802; TUNCER, Purhan Barbaros -- 0000-0002-9257-9544
dc.contributor.authorKutluyer, Filiz
dc.contributor.authorKayim, Murathan
dc.contributor.authorOgretmen, Fatih
dc.contributor.authorBüyükleblebici, Serhat
dc.contributor.authorTuncer, Pürhan Barbaros
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:26:28Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:26:28Z
dc.date.issued2014
dc.departmentVeteriner Fakültesi
dc.description.abstractIn present study, it was examined whether addition of different antioxidants to the cryopreservation extenders had an effect on semen post-thaw fertility and motility in rainbow trout (Oncorhynchus mykiss) and also it was investigated the sperm characteristics post-thaw sperm characteristics and fertility. The collected semen was pooled to minimize individual variation. Each pooled ejaculate was split into 12 equal aliquots and diluted with base extenders supplemented with the antioxidants, and a base extender with no additives (control). The pooled semen samples diluted at the ratio of 1:10 by the extenders were subjected to cryopreservation. Antioxidants were separately added to the extenders (one per experimental group): catalase (250 U/l), superoxide dismutase (250 U/l), peroxidase (250 U/l), oxidized glutathione (1.5 mmol/l), reduced glutathione (1.5 mmol/l), L-methionine (1.5 mmol/l), uric acid (0.25 mmol/l), L-ascorbic acid (0.5 mmol/l), alpha-tocopherol (2.0 mmol/l), beta-carotene (0.5 mmol/l) and camitine (0.5 mmol/l). After dilution the semen was aspirated into 0.25 ml straws, the straws were placed on the tray, frozen for 10 min, and plunged into liquid nitrogen. Our results indicated that the post-thaw motility rate increased in extenders supplemented with uric acid, L-methionine, SOD, L-carnitine, alpha-tocopherol and L-reduced glutathione (p < 0.05). The motility duration of frozen thawed semen increased in extenders supplemented with uric acid, L-methionine, SOD, alpha-tocopherol and L-reduced glutathione (p < 0.05). Fertilization rate and hatching rate of frozen-thawed semen was not affected by the tested antioxidants. Consequently, the tested antioxidants affected the motility parameters and cryopreservation extenders could be supplement with antioxidants. This study suggested usage of antioxidants in the cryopreservation of rainbow trout.
dc.description.sponsorshipScientific and Technological Research Council of Turkey [TUBITAK-TOVAG 1120285]
dc.description.sponsorshipThis study was carried out as a part of a research project, "Determination of the effect of antioxidants on the quality of cryopreserved semen in Rainbow trout (Oncorhynchus mykiss) and determination of fatty acids, protein profiles and seminal plasma composition" supported by the Scientific and Technological Research Council of Turkey (Project No: TUBITAK-TOVAG 1120285).
dc.identifier.doi10.1016/j.cryobiol.2014.10.005
dc.identifier.endpage466en_US
dc.identifier.issn0011-2240
dc.identifier.issn1090-2392
dc.identifier.issue3en_US
dc.identifier.pmid25445462
dc.identifier.scopusqualityQ1
dc.identifier.startpage462en_US
dc.identifier.urihttps://doi.org/10.1016/j.cryobiol.2014.10.005
dc.identifier.urihttps://hdl.handle.net/20.500.12451/5606
dc.identifier.volume69en_US
dc.identifier.wosWOS:000346461500015
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAcademic Press
dc.relation.ispartofCRYOBIOLOGY
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectRainbow Trout
dc.subjectOncorhynchus Mykiss
dc.subjectAntioxidant
dc.subjectSperm Quality
dc.titleCryopreservation of rainbow trout oncorhynchus mykiss spermatozoa: effects of extender supplemented with different antioxidants on sperm motility, velocity and fertility
dc.typeArticle

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