Cryopreservation of bull sperm: Effects of extender supplemented with different cryoprotectants and antioxidants on sperm motility, antioxidant capacity and fertility results

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dc.authoridTUNCER, Purhan Barbaros -- 0000-0002-9257-9544
dc.contributor.authorBüyükleblebici, Serhat
dc.contributor.authorTuncer, Pürhan Barbaros
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorEken, Ayşe
dc.contributor.authorSarıözkan, Serpil
dc.contributor.authorTaşdemir, Umut
dc.contributor.authorEndirlik, Burcu Ünlü
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:26:48Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:26:48Z
dc.date.issued2014
dc.departmentVeteriner Fakültesi
dc.description.abstractThe objectives of this study were to compare glycerol (G) and ethylene glycol (EG) at different concentrations and trehalose (T) or cysteine (C; with/without) in Tris extender for cryopreservation of bull semen. Twenty-four ejaculates obtained from three bulls were included in the study. Each ejaculate was divided into four equal aliquots and diluted using both of the Tris extenders with G (5% or 7%) or EG (3% or 5%). After that, each extenders were divided into three equal aliquots and diluted using both of the 5 mM C or 25 mM T, and control (without additives) was cooled to 4 degrees C and frozen in 0.25 ml French straws. The addition of 3% and 5% EG without antioxidants resulted in the least Computer-Assisted Sperm motility Analysis (CASA) motility as compared with the other groups. Treatment with 25 mM Tin 3% EG beneficially effected acrosome morphology as compared with the other groups. Also, treatment with 3% EG with 25 mM T and 5% EG resulted in a greater rate of total abnormalities. Treatment with 3% G yielded a slightly greater percentage of membrane integrity by Hypo-Osmotic Swelling Test (HOST) assessment than that of the other groups. Treatment with 3% EG with 5 mM C resulted in the greatest concentration of malondialdehyde (MDA). The glutathione peroxidase (GPx) antioxidant activity was increased in the C-treatment groups when compared to the other groups. Treatment with 5% EG and 5 mM C resulted in less chromatin damage and detrimental impacts on tail moment. Treatment with 5% EG led to greater non-return rates of inseminated cows. However, this result was not considered to be statistically important.
dc.description.sponsorshipRepublic of Turkey, Ministry of Agriculture and Rural Affairs, General Directorate of Agricultural Research (GDAR) [09/01/01/01]
dc.description.sponsorshipThis study was supported by the Republic of Turkey, Ministry of Agriculture and Rural Affairs, General Directorate of Agricultural Research (GDAR) Project number: 09/01/01/01.
dc.identifier.doi10.1016/j.anireprosci.2014.09.006
dc.identifier.endpage83en_US
dc.identifier.issn0378-4320
dc.identifier.issn1873-2232
dc.identifier.issue03.Apren_US
dc.identifier.pmid25278438
dc.identifier.scopusqualityQ1
dc.identifier.startpage77en_US
dc.identifier.urihttps://doi.org/10.1016/j.anireprosci.2014.09.006
dc.identifier.urihttps://hdl.handle.net/20.500.12451/5714
dc.identifier.volume150en_US
dc.identifier.wosWOS:000345476200001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bv.
dc.relation.ispartofAnimal Reproduction Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAntioxidants
dc.subjectBull semen
dc.subjectCryoprotectant
dc.subjectFreezing
dc.subjectFertility
dc.titleCryopreservation of bull sperm: Effects of extender supplemented with different cryoprotectants and antioxidants on sperm motility, antioxidant capacity and fertility results
dc.typeArticle

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