DNA damaging effect of paclitaxel in the epididymal sperms as a chemotherapeutic agent and possible remedies to prevent this effect: A study on reproductive potential of male cancer patients of reproductive age
Yükleniyor...
Dosyalar
Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Ltd.
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Cancer is a major public health problem, young cancer patients therefore undergo chemotherapy, andmost of them may lose their fertility. DNA damage level provides important clues about the quality andreproductive potential of spermatozoa. In this study, we evaluated the levels of both DNA fragmentationand abnormal DNA integrity in the epididymal sperms of New Zealand rabbit (Oryctolagus cuniculus)after cryopreservation using the terminal deoxyribonucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) assay and the toluidine blue (TB) staining methods and assessed the effects of pacli-taxel, resveratrol,L-glutamine (LG), and basal medium eagle (BME) solution on DNA damage. Paclitaxelinduced the levels of both DNA damages in the sperms, but resveratrol ameliorated this effect. LG andBME supplementation to the extender prevented the sperm samples from DNA fragmentation aftercryopreservation. Chemotherapy drugs containing paclitaxel can cause the sperm DNA to be damaged,and hence adversely affect the fertility of male cancer patients of reproductive age. The administration ofresveratrol together with paclitaxel may ameliorate the DNA damage inducing effect of paclitaxel. Spermbanking and cryopreservation with the appropriate cryoprotectants such as LG and BME prior to cancertreatment can also be suggested to all male cancer patients of reproductive age facing cancer treatmentfor fertility preservation.
...
...
Açıklama
Öztürk, Caner ( Aksaray, Yazar )
Anahtar Kelimeler
Paclitaxel, Resveratrol, DNA Damage, Cryopreservation, Cancer, Fertility
Kaynak
Theriogenology
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
132
