A novel adsorbent for protein chromatography: Supermacroporous monolithic cryogel embedded with Cu2+-attached sporopollenin particles

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dc.contributor.authorErzengin, Mahmut
dc.contributor.authorÜnlü, Nuri
dc.contributor.authorOdabaşı, Mehmet
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:26:18Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:26:18Z
dc.date.issued2011
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractThe aim of this study is to prepare supermacroporous cryogels embedded with Cu2+-attached sporopollenin particles (Cu2+-ASP) having large surface area for high protein adsorption capacity. Super-macroporous poly(2-hydroxyethyl methacrylate) (PHEMA)-based monolithic cryogel column embedded with Cu2+-ASP was prepared by radical cryo-copolymerization of 2-hydroxyethyl methacrylate (HEMA) with N,N'-methylene-bis-acrylamide (MBAAm) as cross-linker directly in a plastic syringe for affinity purification of human serum albumin (HSA). Firstly, Cu2+ ions were attached to sporopollenin particles (SP), then the supermacroporous PHEMA cryogel with embedded Cu2+-ASP was produced by free radical polymerization using N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) as initiator/activator pair in an ice bath. Embedded particles (10 mg) in PHEMA-based cryogel column were used in the adsorption/desorption of HSA from aqueous solutions. Optimum conditions of adsorption experiments were performed at pH 8.0 phosphate buffer, with flow rate of 0.5 mL/min, and at 5 degrees C. The maximum amount of HSA adsorption from aqueous solution was very high (677.4 mg/g SP) with initial concentration 6 mg/mL It was observed that HSA could be repeatedly adsorbed and desorbed to the embedded Cu2+-ASP in PHEMA cryogel without significant loss of adsorption capacity. (C) 2010 Elsevier B.V. All rights reserved.
dc.identifier.doi10.1016/j.chroma.2010.11.074
dc.identifier.endpage490en_US
dc.identifier.issn0021-9673
dc.identifier.issue3en_US
dc.identifier.pmid21176840
dc.identifier.scopusqualityQ1
dc.identifier.startpage484en_US
dc.identifier.urihttps://doi.org/10.1016/j.chroma.2010.11.074
dc.identifier.urihttps://hdl.handle.net/20.500.12451/5530
dc.identifier.volume1218en_US
dc.identifier.wosWOS:000286680900015
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Ltd.
dc.relation.ispartofJournal of Chramatography A
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectProtein Adsorption
dc.subjectCryogels
dc.subjectSporopollenin
dc.subjectMetal Chelate
dc.subjectParticle Embedding
dc.titleA novel adsorbent for protein chromatography: Supermacroporous monolithic cryogel embedded with Cu2+-attached sporopollenin particles
dc.typeArticle

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