A novel adsorbent for protein chromatography: Supermacroporous monolithic cryogel embedded with Cu2+-attached sporopollenin particles
Yükleniyor...
Tarih
2011
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Ltd.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The aim of this study is to prepare supermacroporous cryogels embedded with Cu2+-attached sporopollenin particles (Cu2+-ASP) having large surface area for high protein adsorption capacity. Super-macroporous poly(2-hydroxyethyl methacrylate) (PHEMA)-based monolithic cryogel column embedded with Cu2+-ASP was prepared by radical cryo-copolymerization of 2-hydroxyethyl methacrylate (HEMA) with N,N'-methylene-bis-acrylamide (MBAAm) as cross-linker directly in a plastic syringe for affinity purification of human serum albumin (HSA). Firstly, Cu2+ ions were attached to sporopollenin particles (SP), then the supermacroporous PHEMA cryogel with embedded Cu2+-ASP was produced by free radical polymerization using N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) as initiator/activator pair in an ice bath. Embedded particles (10 mg) in PHEMA-based cryogel column were used in the adsorption/desorption of HSA from aqueous solutions. Optimum conditions of adsorption experiments were performed at pH 8.0 phosphate buffer, with flow rate of 0.5 mL/min, and at 5 degrees C. The maximum amount of HSA adsorption from aqueous solution was very high (677.4 mg/g SP) with initial concentration 6 mg/mL It was observed that HSA could be repeatedly adsorbed and desorbed to the embedded Cu2+-ASP in PHEMA cryogel without significant loss of adsorption capacity. (C) 2010 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
Protein Adsorption, Cryogels, Sporopollenin, Metal Chelate, Particle Embedding
Kaynak
Journal of Chramatography A
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
1218
Sayı
3
