Efficient adsorption of hemoglobin from aqueous solutions by hybrid monolithic cryogel column

dc.contributor.authorYılmaz Baran, Nuray
dc.contributor.authorAcet, Ömür
dc.contributor.authorOdabaşı, Mehmet
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:28:45Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:28:45Z
dc.date.issued2017
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractIn this study, a supermacroporous poly(2-hydroxyethyl methacrylate) (PHEMA)-based Cu2+-attached bentonite particles embedded hybrid monolithic cryogel (Cu2+-ABPs EHMC) was prepared by radical cryo-copolymerization of HEMA with N,N'-methylene-bis-acrylamide (MBAAm) as a cross-linker directly in a plastic syringe in the presence of Cu2+-ABPs, and used for hemoglobin (Hb) separation from aqueous solution. Due to the naturally abundant hydroxyl groups on bentonite, Cu2+-attached bentonite particles (Cu2+-ABPs) embedded hybrid monolithic cryogel (HMC) showed a good adsorption performance for Hb, covering an excellent adsorption capacity of 521.6 mg/g bentonite in phosphate buffer at pH 6.0 with a flow rate of 0.5 mL/min, fast binding stability within 60 min, well selectivity and reversibility. Compared with other adsorbent systems Used for protein separation, this sorbent has many advantages such as excellent adsorption performance, ease of preparation, suitable, effective and environment-friendly to perform, which can serve as a more sustainable material in protein separation and purification. (C) 2016 Elsevier B.V. All rights reserved.
dc.identifier.doi10.1016/j.msec.2016.12.036
dc.identifier.endpage20en_US
dc.identifier.issn0928-4931
dc.identifier.issn1873-0191
dc.identifier.pmid28183592
dc.identifier.scopusqualityN/A
dc.identifier.startpage15en_US
dc.identifier.urihttps://doi.org/10.1016/j.msec.2016.12.036
dc.identifier.urihttps://hdl.handle.net/20.500.12451/6080
dc.identifier.volume73en_US
dc.identifier.wosWOS:000394064800003
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofMaterials Science & Engineering C-Materials For Biological Applications
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBentonite
dc.subjectGel Electrophoresis
dc.subjectHemoglobin Adsorption
dc.subjectHybrid Cryogel
dc.subjectMetal Chelate
dc.subjectParticle Embedding
dc.titleEfficient adsorption of hemoglobin from aqueous solutions by hybrid monolithic cryogel column
dc.typeArticle

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