Efficient adsorption of hemoglobin from aqueous solutions by hybrid monolithic cryogel column
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Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier Science Bv
Access Rights
info:eu-repo/semantics/openAccess
Abstract
In this study, a supermacroporous poly(2-hydroxyethyl methacrylate) (PHEMA)-based Cu2+-attached bentonite particles embedded hybrid monolithic cryogel (Cu2+-ABPs EHMC) was prepared by radical cryo-copolymerization of HEMA with N,N'-methylene-bis-acrylamide (MBAAm) as a cross-linker directly in a plastic syringe in the presence of Cu2+-ABPs, and used for hemoglobin (Hb) separation from aqueous solution. Due to the naturally abundant hydroxyl groups on bentonite, Cu2+-attached bentonite particles (Cu2+-ABPs) embedded hybrid monolithic cryogel (HMC) showed a good adsorption performance for Hb, covering an excellent adsorption capacity of 521.6 mg/g bentonite in phosphate buffer at pH 6.0 with a flow rate of 0.5 mL/min, fast binding stability within 60 min, well selectivity and reversibility. Compared with other adsorbent systems Used for protein separation, this sorbent has many advantages such as excellent adsorption performance, ease of preparation, suitable, effective and environment-friendly to perform, which can serve as a more sustainable material in protein separation and purification. (C) 2016 Elsevier B.V. All rights reserved.
Description
Keywords
Bentonite, Gel Electrophoresis, Hemoglobin Adsorption, Hybrid Cryogel, Metal Chelate, Particle Embedding
Journal or Series
Materials Science & Engineering C-Materials For Biological Applications
WoS Q Value
N/A
Scopus Q Value
N/A
Volume
73
