Safety and metabolic characteristics of 17 Enterococcus faecium isolates

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dc.authorid0000-0002-0381-5876
dc.contributor.authorYüksekdağ, Zehranur
dc.contributor.authorAhlatçı, Nur Seda
dc.contributor.authorHajikhani, Rasta
dc.contributor.authorÖnal Darılmaz, Derya
dc.contributor.authorBeyatlı, Yavuz
dc.date.accessioned2021-12-07T07:27:13Z
dc.date.available2021-12-07T07:27:13Z
dc.date.issued2021
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractIn the present study, metabolic characteristics, such as lactic acid, hydrogen peroxide, exopolysaccharide (EPS) production, and antimicrobial activities, of 17 Enterococcus faecium isolates from white cheese samples were assessed. In E. faecium isolates, the amount of lactic acid obtained between in MRS medium 0.61–1.22% and in skim milk 0.75–1.08%, and the amount of H2O2 was found between 0.57 and 3.17 µg mL?1. In MRS and skim milk, the amount of EPS production was 59–185 mg L?1, 155–255 mg L?1 for isolates, respectively. The antimicrobial activities of E. faecium isolates on eight different pathogenic bacteria were also performed by an agar well diffusion method. The highest inhibition zones 8.60 mm were observed with culture supernatants of RI-71 isolate against Escherichia coli ATCC 35218. The safety of the E. faecium isolates was assessed by determining gelatinase activity, hemolytic activity, the resistance to ten different antibiotics, biofilm forming, and virulence genes (van A, van B, gelE, cylA, cylB, esp, agg, and asa1, efaAfm, cob, ccf, hyl). The isolates did not show gelatinase activity, ?-hemolysis, and biofilm formation. All E. faecium isolates were susceptible to vancomycin, penicillin-G, tetracycline, ampicillin, and chloramphenicol. The efaAfm gene was detected most frequently (94%) followed by cob (82%), van B (59%), and ccf (53%). For enterococci to be recommended as co-starter or probiotic adjunct cultures, it is necessary to determine whether they have virulence genes and resistance to antibiotics.
dc.identifier.doi10.1007/s00203-021-02536-8
dc.identifier.endpage5694en_US
dc.identifier.issn0302-8933
dc.identifier.issue9en_US
dc.identifier.pmid34468805
dc.identifier.scopusqualityQ3
dc.identifier.startpage5683en_US
dc.identifier.urihttps:/dx.doi.org/10.1007/s00203-021-02536-8
dc.identifier.urihttps://hdl.handle.net/20.500.12451/8875
dc.identifier.volume203en_US
dc.identifier.wosWOS:000691968500001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer Science and Business Media Deutschland GmbH
dc.relation.ispartofArchives of Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectAntibiotic Susceptibility
dc.subjectEnterococcus Faecium
dc.subjectExopolysaccharide
dc.subjectHydrogen Peroxide
dc.subjectLactic Acid
dc.subjectVirulence Genes
dc.titleSafety and metabolic characteristics of 17 Enterococcus faecium isolates
dc.typeArticle

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