Safety and metabolic characteristics of 17 Enterococcus faecium isolates
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Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
Springer Science and Business Media Deutschland GmbH
Access Rights
info:eu-repo/semantics/embargoedAccess
Abstract
In the present study, metabolic characteristics, such as lactic acid, hydrogen peroxide, exopolysaccharide (EPS) production, and antimicrobial activities, of 17 Enterococcus faecium isolates from white cheese samples were assessed. In E. faecium isolates, the amount of lactic acid obtained between in MRS medium 0.61–1.22% and in skim milk 0.75–1.08%, and the amount of H2O2 was found between 0.57 and 3.17 µg mL?1. In MRS and skim milk, the amount of EPS production was 59–185 mg L?1, 155–255 mg L?1 for isolates, respectively. The antimicrobial activities of E. faecium isolates on eight different pathogenic bacteria were also performed by an agar well diffusion method. The highest inhibition zones 8.60 mm were observed with culture supernatants of RI-71 isolate against Escherichia coli ATCC 35218. The safety of the E. faecium isolates was assessed by determining gelatinase activity, hemolytic activity, the resistance to ten different antibiotics, biofilm forming, and virulence genes (van A, van B, gelE, cylA, cylB, esp, agg, and asa1, efaAfm, cob, ccf, hyl). The isolates did not show gelatinase activity, ?-hemolysis, and biofilm formation. All E. faecium isolates were susceptible to vancomycin, penicillin-G, tetracycline, ampicillin, and chloramphenicol. The efaAfm gene was detected most frequently (94%) followed by cob (82%), van B (59%), and ccf (53%). For enterococci to be recommended as co-starter or probiotic adjunct cultures, it is necessary to determine whether they have virulence genes and resistance to antibiotics.
Description
Keywords
Antibiotic Susceptibility, Enterococcus Faecium, Exopolysaccharide, Hydrogen Peroxide, Lactic Acid, Virulence Genes
Journal or Series
Archives of Microbiology
WoS Q Value
Q4
Scopus Q Value
Q3
Volume
203
Issue
9
