Cytotoxic and apoptotic activities of novel Pd(II) complexes against human leukemia cell lines in vitro

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dc.authoridBARAN, TALAT -- 0000-0003-0206-3841
dc.contributor.authorTekin, Neslihan
dc.contributor.authorÖztürk, Kamile
dc.contributor.authorBaran, Talat
dc.contributor.authorKerimoğlu, Barış
dc.contributor.authorTarhan, Mehtap
dc.contributor.authorMenteş, Ayfer
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:15:29Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:15:29Z
dc.date.issued2017
dc.departmentSabire Yazıcı Fen-Edebiyat Fakültesi
dc.description.abstractIn the investigation for alternative chemotherapeutic strategies against leukemia, Pd(II) complexes were synthesized and investigated for cytotoxic and apoptotic properties on two human leukemia cell lines (HL-60 and K562). Pd(II) complexes (Pd-5a and Pd-6a) with 5a and 6a as ligands were synthesized and characterized by H-1-NMR and F-TIR. The cytotoxicity of the compounds was quantified using MTT method. Bax, Bcl-2, and caspase 3 gene expression levels were estimated using RT-qPCR. Here we show that Pd(II) complexes have important cytotoxic activity on human leukemia cell lines. RT-qPCR indicated that Bax and caspase 3 gene expression levels were increased after 24h treatment with Pd-5a and Pd-6a complexes in both HL-60 and K562 cells at some selected dose. Furthermore, Bcl-2 gene expression level decreased after 24h treatment with Pd-5a and Pd-6a complexes in K562 cells at all selected dose. In HL-60 cells, only one selected Pd-5a dose (25 mu M) decreased the gene expression level of Bcl-2. The results obtained in the present investigation indicate that these two newly synthesized Pd(II) complexes have apoptotic effects at appropriate doses through caspase 3 and Bax genes and might represent a novel potentially active agents for the management of human leukemia cell lines.
dc.description.sponsorshipAksaray University Scientific Research Fund [2014-009]
dc.description.sponsorshipThis study was supported by Aksaray University Scientific Research Fund, grant number 2014-009.
dc.identifier.doi10.1080/10601325.2017.1294449
dc.identifier.endpage270en_US
dc.identifier.issn1060-1325
dc.identifier.issn1520-5738
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage263en_US
dc.identifier.urihttps://doi.org/10.1080/10601325.2017.1294449
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4397
dc.identifier.volume54en_US
dc.identifier.wosWOS:000399809000001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTaylor & Francis
dc.relation.ispartofJournal of Macromolecular Science, Part A. Pure and Applied Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectApoptosis
dc.subjectBax
dc.subjectBcl-2
dc.subjectCaspase 3
dc.subjectLeukemia
dc.subjectPalladium (II) Complexes
dc.titleCytotoxic and apoptotic activities of novel Pd(II) complexes against human leukemia cell lines in vitro
dc.typeArticle

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