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Öğe Combination of cysteamine and lipoic acid ımproves the post-thawed bull sperm parameters(2016) Güngör, Şükrü; Aksoy, Adil; Yeni, Deniz; Avdatek, Fatih; Öztürk, Caner; Ataman, Mehmet Bozkurt; Coyan, Kenan; Bucak, Mustafa NumanThe present study was conducted to examine the protective roles of cysteamine, trehalose, alpha-lipoic acid and combinations of these antioxidants on post-thawed bull sperm and oxidative stress parameters. Five healthy Holstein bull (3-4 years old) were used. Eight ejaculates for each bull were collected and pooled. Pooled ejaculate, splitted into seven equal aliquots and diluted at 37 °C with base extenders containing cysteamine 2 mM, trehalose 50 mM, alpha-lipoic acid (ALA) 1 mM, cysteamine 2 mM + trehalose 50 mM, ALA 1 mM + trehalose 50 mM, cysteamine 2 mM + ALA 1 mM and no antioxidant (control), was cooled to 5 °C and then frozen. Frozen straws were thawed in a water bath for evaluation. The combination of cysteamine 2 mM and ALA 1 mM of the semen extender improved the percentages of post-thawed subjective motility (68 ± 2.7%), and progressive motility (42.9 ± 4.7%), compared with the controls (61 ± 4.2% and 37.5 ± 8%, respectively, non- significantly, P>0.05). The supplementation of the semen extender with combination of cysteamine 2 mM and ALA 1 mM produced a higher acrosome integrity and mitochondrial activity (52.02 ± 6.4% and 32 ± 4.1%, respectively), compared with the controls (30.5 ± 1.7 and 14.02 ± 3.5% respectively, P < 0.05). Combination of cysteamine and ALA antioxidants in semen extenders provided the benefit in terms of sperm motilities, acrosome integrity and mitochondrial activity on frozen-thawed bull spermÖğe Cryopreservation of Ram Semen Using Capsaicin Supplemented Tris Extender(Afyon Kocatepe Üniversitesi, 2023) İnanç, Muhammed Enes; Avdatek, Fatih; Güngör, Şükrü; Gülhan, Mehmet Fuat; Olgaç, Kemal Tuna; Denk, Barış; Yeni, Deniz; Taşdemir, UmutIn this study, it was designed to reveal the effects of capsaicin on oxidative stress and freezability of ram semen. Ejaculates were taken from Sönmez rams and divided into five specimens and diluted with extender at different rates (4 mM, 2 mM, 1 mM, 500 ?M) with and without capsaicin (control; C). Semen samples were thawed with a 37°C water bath for 30 seconds for post-thawed analysis. At the end of the study, sperm motility and kinetic parameters, plasma membrane acrosome integrity (PMAI), mitochondrial membrane potential (MMP), DNA damage, oxidant and antioxidant parameters were analyzed. A decrease was observed in the groups containing capsaicin compared to the C in terms of progressive, total motility and kinetic parameters (p<0.05). Besides, positive results were not obtained DNA integrity, PMAI and MMP (p<0.05). In conclusion; it was determined that capsaicin added to Tris extender did not have a positive effect on oxidative stress and freezing of ram semen.Öğe Cryopreservation of ram semen: baicalein efficiency on oxidative stress, chromatin integrity, viability and motility post thaw(Frontiers Media SA, 2024) Avdatek, Fatih; Güngör, Şükrü; Gülhan, Mehmet Fuat; İnanç, Muhammed Enes; Olğaç, Kemal Tuna; Denk, Barış; Yeni, Deniz; Taşdemir, UmutBaicalein (B) has potential antioxidant properties, but it has not been tested as a ram semen extender. This study aimed to assess the impact of B on various sperm parameters and determine its potential influence on semen quality after the freeze-thawing process. During the breeding season, ejaculates were obtained from four rams with the aid of an artificial vagina. The collected mixed semen samples were divided into four groups: control (C; 0), B0.5 (0.5 mM), B1 (1 mM), and B2 (2 mM). After semen extension, the samples were loaded into 0.25 mL straws and stored for 2 h at 4°C prior to freezing in liquid nitrogen vapor and thawed in a water bath at 37°C. Among the groups, B0.5 demonstrated the highest progressive motility results, while B1 and B2 exhibited reduced motility (p < 0.05). In terms of high mitochondrial membrane potential, plasma membrane and acrosome integrity, and viability, B0.5 showed significantly superior outcomes to the other B groups (p < 0.05), although it was not significantly better than C. B1 displayed the highest plasma membrane integrity levels (p < 0.05). Notably, B2 displayed the lowest total antioxidant status levels among the groups (p < 0.05). The findings of this study suggested that the in vitro spermatological characteristics of ram spermatozoa such as progressive motility and chromatin integrity can be protected from the freeze-thawing process by using the 0.5 mM dose of baicalein as a semen extender. The treatment of sperm freezing might benefit from further in-depth research on the role of B in the improvement of cryoinjury and its underlying processes.Öğe Cryoprotective effect of vaccenic acid supplemented in bull semen extender(Scientific and Technological Research Council of Turkey, 2022) Avdatek, Fatih; Taşdemir, Umut; İnanç, Muhammed Enes; Yeni, Deniz; Gülhan, Mehmet Fuat; Güngör, Şükrü; Türkmen, Ruhi; Tuncer, Purhan BarbarosThe aim of this study was to investigate whether vaccenic acid (VA) added to TRIS semen extender has an effect on freezing bull semen. The semen was collected at intervals of three days through an artificial vagina then pooled. The pooled ejaculates were divided into five components as the control (C; no supplement) and VA (25, 50, 100, and 200 mu g / mL) groups then extended with the TRIS-based medium. The samples were left in a cold air cabinet for cooling process (4 degrees C), then they were equilibrated for 4 h. Afterward, the samples in the groups were frozen by using a programmable freezer. The greatest progressive and total motility results were obtained in the VA25 group (p < 0.001). Besides, the total abnormality and chromatin damage was determined to be on the lowest level in the VA25 group (p < 0.05). Except for VA200, the glutathione level was greater in the treatment groups in comparison to the control group (p < 0.05), while the lowest malondialdehyde was determined in VA25. In conclusion, VA supplemented with TRIS-based extender at the concentration of 25 mu g / mL improves the progressive and total motility properties of spermatozoa, reduces the abnormal spermatozoa rate, and has a positive effect on chromatin integrity.Öğe Evaluation of the in vitro cryopreservative performance of Juniper berry oil (Juniperus communis) on frozen-thawed bull semen(Acta Veterinaria Brno, 2023) Güngör, Şükrü; Yeni, Deniz; İnanç, Muhammed Enes; Türkmen, Ruhi; Tuncer, Pürhan Barbaros; Gülhan, Mehmet Fuat; Avdatek, Fatih; Taşdemir, UmutThis study aimed to reveal the effects of juniper (Juniperus communis) berry oil (JB) when added to the Tris extender, on bull (Holstein) sperm motility characteristics, abnormal sperm ratio, DNA fragmentation and some oxidative stress markers. The pooled ejaculate was divided into five equal parts as control (C) and Tris diluent containing 25, 50, 100, 200 ?g/ml JB. Diluted semen was incubated at 4 °C for 4 h and then frozen in liquid nitrogen (?196 °C). After freezing-thawing, motility characteristics were determined using a sperm analyzer system. The results showed that the 25 ?g/ml dose of JB had an improving effect on overall sperm motility (P < 0.05). The total abnormal spermatozoa ratio was not significantly different between the groups (P > 0.05). The JB25 and JB50 groups had higher DNA integrity than the other groups (P < 0.05). This finding was supported by low malondialdehyde levels (P < 0.05). Especially the 25 ?g/ml dose of JB was found to have a remedial effect on DNA integrity, as well as increasing progressive and total motility rates. This study has revealed new information on the effects of JB on bull semen cryopreservation. Juniper berry oil may be used as an antioxidant in bull semen freezing.Öğe Investigation of changes in spermatozoon characteristics, chromatin structure, and antioxidant/oxidant parameters after freeze-thawing of hesperidin (Vitamin P) doses added to ram semen(MDPI, 2022) Yeni, Deniz; Güngor, Şükrü; Avdatek, Fatih; Gülhan, Mehmet Fuat; Olgaç, Kemal Tuna; İnanç, Muhammed Enes; Denk, Barış; Taşdemir, UmutWe conducted this study to determine the potential cryopreservative effects of different hesperidin (vitamin P; H) doses on ram semen after freeze-thawing. Semen samples were obtained from Sonmez rams using an artificial vagina. The samples were divided into six groups: control, 10, 50, 100, 250, and 500 mu g/mL H (C, H10, H50, H100, H250, and H500, respectively). At the end of the study, sperm motility and kinetic parameters, acrosome integrity (AI), mitochondrial membrane potential (MMP), viability, lipid peroxidation levels (LPL), chromatin damage, oxidant parameters, and antioxidant parameters were assayed. None of the doses of H added to the semen extender showed any enhancing effects on progressive motility compared to C (p > 0.05). In fact, H500 had negative effects (p < 0.05). Moreover, AI was the highest at the H10 dose, while LPL values were the lowest at the same dose (p < 0.05). The doses of H10 and H50 added to the Tris extender medium showed positive effects on sperm cell chromatin damage. Consequently, we can say that H doses used in this study are not effective on semen progressive motility, but the H10 dose is effective on AI and chromatin damage by reducing LPL.Öğe Investigation of the effects of syringic acid supplemented to Tris semen diluent on ram semen freezability(John Wiley and Sons Inc, 2023) Avdatek, Fatih; İnanç, Muhammed Enes; Gülhan, Mehmet Fuat; Güngör, Şükrü; Yeni, Deniz; Olğaç, Kemal Tuna; Denk, Barış; Taşdemir, UmutTo the best of our knowledge, no research has been conducted to test the effects of syringic acid (SA) on ram semen freezing within the scope of natural antioxidants added to semen extenders. Therefore, this study had two main objectives. First, to test whether adding SA to ram semen freezing extender has a protective effect and contributes positively to sperm kinetic, plasma and acrosome integrity, mitochondrial membrane potential, lipid peroxidation, oxidant and antioxidant and DNA damage parameters after thawing. Second, it was to determine at what concentration the SA supplemented to the extender could be applied by in vitro studies by preserving the fertilization ability of frozen semen at the highest level. In the study, six individuals of Sönmez rams were used. The semen was collected from the rams using an artificial vagina and pooled. The pooled semen was divided into five different groups and extended with 0, 0.5, 1, 2 and 4 mM SA (control C, SA0.5, SA1, SA2 and SA4, respectively). After dilution, the semen samples were kept at 4°C for 3 h, then loaded into 0.25 mL straws and frozen in liquid nitrogen vapour. The SA1 and SA2 groups were higher plasma membrane and acrosome integrity (PMAI), high mitochondrial membrane potential (HMMP), plasma membrane integrity and motility compared to other groups (p <.05). It was observed that SA supplemented to the Tris extender significantly reduced DNA damage, and the lowest values were obtained especially in the SA1 and SA2 treatments (p <.05). Also, lowest MDA level was determined at the SA1 and this was statistically significant compared to SA4 and C (p [removed]Öğe Potential protective effect of hesperidin (Vitamin P) against hlyphosate-induced spermatogenesis damage in male rats: biochemical and histopathological findings on reproductive parameters(MDPI, 2024) Güngör, Şükrü; Kırıkkulak, Murat; Denk, Barış; Gülhan, Mehmet Fuat; Güleş, Özay; Budak, Duygu; İnanç, Muhammed Enes; Avdatek, Fatih; Yeni, Deniz; Taşdemir, UmutThe aim of this study was to investigate the effectiveness of hesperidin (HES) on testicular histopathological changes, biochemical changes, and semen characteristics in rats exposed to glyphosate (GLP). The control group was given a normal diet devoid of GLP and HES, the HES group was given 100 mg/kg/day HES with the normal diet, the GLP group was given GLP at the LD50/10 dose of normal feed, which was 787.85 mg/kg/day, and the GLP + HES group was given normal feed containing 787.85 mg/kg/day LD50/10 dose of GLP in addition to 100 mg/kg/day HES. GLP administration reduced sperm motility, sperm plasma membrane integrity, glutathione levels, and total antioxidant levels in the testicular tissues of rats. Moreover, it caused an increase in right testis and left epididymis weights, abnormal sperm counts, malondialdehyde levels, total oxidant status, and DNA damage. The HES treatment showed curative effects on these parameters. Furthermore, HES was effective in lessening the histopathological damage that was caused by GLP. The results showedthat HES protects spermatological parameters and DNA integrity, improves antioxidant defenses, and lowers the damage and lipid peroxidation caused by GLP in testicular tissue.Öğe Red pine (Pinus brutia Ten) bark tree extract preserves sperm quality by reducing oxidative stress and preventing chromatin damage(Blackwell Publishing Ltd, 2020) Taşdemir, Umut; Yeni, Deniz; İnanç, Muhammed Enes; Avdatek, Fatih; Çil, Beste; Türkmen, RuhiThis study aimed to investigate the effectiveness of using red pine bark tree extract (P; Pinus brutia Ten) as a TRIS extender in an attempt to prevent oxidative stress in bull spermatozoa after freezing. Semen specimens were obtained from Simmental bulls via an artificial vagina and pooled. They were separated into five specimens and diluted with Tris extender consisting of P (200, 100, 50 and 25 µg/ml) and P free (control; C) up to a final concentration of 16 × 106 per straw. All specimens were equilibrated for a period of 4 hr at a temperature of 4°C, following which they were filled in 0.25-ml French straws and frozen. Addition of P resulted in favourable tail length in comparison with C (p <.05). The lowest malondialdehyde levels and the highest glutathione levels were detected in all P groups (p <.05). Supplementation with P did not show advanced results in terms of total, progressive sperm motility and total abnormality in comparison with C (p >.05). In conclusion, it has been shown that although P added to a Tris extender does not have a positive effect on sperm motility, it prevents chromatin damage by reducing oxidative stress, in addition to reducing head abnormalities when used at the amount of 50 ?g/ml.Öğe Supplementation of quercetin for advanced DNA integrity in bull semen cryopreservation(Wiley, 2018) Avdatek, Fatih; Yeni, Deniz; İnanç, Muhammed Enes; Çil, Beste; Tuncer, B. P.; Türkmen, Ruhi; Taşdemir, UmutThe aim of this study was to identify the effects of adding quercetin (Q) to Tris extender in order to identify levels of oxidative stress in bull sperm after freeze thawing. Ejaculates were collected via artificial vagina from Holstein bulls. Semen was divided into five tools and diluted to a final concentration of 15x10(6) spermatozoa/ml with the Tris extender containing Q (25, 50, 100 and 200g/ml) and no additive (control; C). All examples were equilibrated at 4 degrees C during 4hr then were loaded into 0.25-ml straws and frozen using a controlled rate. Sperm motility and motility characteristics were determined using the computer-assisted semen analyser. Sperm membrane integrity was assessed using the hypoosmotic swelling test. Sperm chromatin integrity was investigated using the single cell gel electrophoresis. Total antioxidant capacities were performed colorimetrically. Q supplementation used as an antioxidant did not produce better results in the proportion of sperm progressive and total motility, plasma membrane integrity and sperm abnormalities. Q supplementation exhibited the favourable tail length, tail DNA and tail moment. In conclusion, when whole parameters are considered, Q25 can be added to the Tris extender due to its positive effect on sperm DNA integrity and no adverse effect on the progressive and total motilities of sperm.Öğe Supplementation of rosmarinic acid has reduced oxidative stress on bull spermatozoa followİng the freeze thawing process(Cryo-Letters, 2018) Yeni, Deniz; İnanç, Muhammed Enes; Avdatek, Fatih; Tuncer, Pürhan Barbaros; Çil, Beste; Türkmen, R.; Taşdemir, UmutBACKGROUND: Cryopreservation has a side effect on the motility, chromatin integrity and viability of sperm cells. OBJECTIVE: The present study investigated the effects of supplementation with rosmarinic acid (RA) Tris extender on sperm quality parameters, plasma and acrosome membrane damage, antioxidant enzyme activity and chromatin integrity following the freeze thawing process on bull spermatozoa. MATERIALS AND METHODS: Ejaculates were split into five aliquots and diluted to a final concentration of 15x106 spermatozoa/ml with the Tris extender containing RA (25, 50, 100 and 200 µg/ml) and (control) and then frozen at a controlled rate. RESULT: Treatments did not give better results on the percentages of sperm progressive, total motility and sperm motion characters (P>0.05); however, RA25 and RA50 exhibited favourable chromatin integrity. In conclusion, RA25 and RA50 increased total antioxidant activity. As a consequence, the amount of MDA and chromatin damage were reduced in sperm cells. © CryoLetters.Öğe The effect of gallic acid addition to tris-based extender on frozen bull semen(Kafkas Üniversitesi, 2021) Güngör, Şükrü; İnanç, Muhammed Enes; Avdatek, Fatih; Türkmen, Ruhi; Taşdemir, UmutThis study aimed to determine the probable protective effect of gallic acid (GA) on the spermatological parameters of frozen-thawed bull semen. Ejaculates were collected from four Holstein bulls. The mixed ejaculate was divided into four equal groups as control (0 pg/mL GA), 50, 100 and 200 mu g/mL GA with a Tris-based extender. All extended groups were cooled, equilibrated into a +4 degrees C cold cabinet and loaded into straws. The straws were then frozen and stored In a liquid nitrogen container (-196 degrees C). Subsequently, samples were thawed in a water bath for analyzing motility and kinematic parameters, morphological integrity, DNA damage and biochemical alterations. GA50 (28.76 +/- 0.51%) and GA100 (29.32 +/- 0.31%) had improved progressive motility in comparison to the other groups (P<0.05). The highest total motility (69.71 +/- 0.52%) was detected in GA100 (P<0.05). Besides, the lowest DNA damage and total abnormality values were detected in the GA100 group (P<0.05). GA100 had the highest total antioxidant capacity, as well as the lowest malondialdehyde (MDA) level (P<0.05). In conclusion, GA 50 and 100 mu g/mL protects the progressive motility, morphological and DNA integrity by improving the total antioxidant status from the harmful effects of the freezing and thawing protocol.Öğe The effect of green tea extract supplementation in bull semen cryopreservation(Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 2019) İnanç, Muhammed Enes; Çil, Beste; Yeni, Deniz; Avdatek, Fatih; Orakçı, Durmuş; Tuncer, Purhan Barbaros; Türkmen, Ruhi; Taşdemir, UmutThis study aims to investigate the effect of catechin (CT), green tea extract, as a supplement to Tris extender on semen quality parameters in frozen-thawed of bull sperm. Ejaculates were taken with artificial vagina from Holstein bulls and divided equal five aliquots, diluted to containing different amounts of CT (5, 10, 25 and 50 mu g/mL) and no-additive (control). All samples were equilibrated at 4 degrees C for 4 h and were frozen using a digital freezing machine. Post-thawed sperm motility and kinetic parameters were determined using the sperm analyser system. Spermatozoa DNA integrity was evaluated with the single cell gel electrophoresis, abnormal spermatozoa rate was evaluated by fluid fixation test and lipid peroxidation status was evaluated calorimetrically. CT supplementation did not improve motility and kinetic parameters. However, the higher morphological integrity was detected in CT10, 25 and 50 groups compared to control (P<0.05). Regarding chromatin integrity, positive effects of catechin were observed in the treatment groups while in CT 50 group adverse effects were found (P<0.05). Although there was no improvement in malondialdehyde levels, the highest total antioxidant activity was seen in the CT50 group (P<0.05). In conclusion, CT supplementation could be used the protection of morphological and DNA integrity from cryodamage and it has increased the total antioxidant activity depending of the dose in bull semen.Öğe The effects of long-chain fatty acids supplemented to rations during the transition and early lactation periods on reproductive performance in Simmental cattle with low estrus signs(Elsevier, 2023) Budak, Duygu; Taşdemir, Umut; Avdatek, FatihThis study is based on the hypothesis that adding rumen bypass unsaturated fatty acids to rations during the transition and early lactation periods will reduce metabolic stress, milk fat synthesis, and milk fat concentration, increase the prevalence of estrus signs and fertilization rates and facilitate the implantation of the embryo. Thus, it was aimed to reveal the effects of calcium soap of long-chain fatty acids (CSFA) added to rations during the transition and early lactation periods in Simmental cattle with low estrus signs. Primiparous and multiparous Simmental cattle were used in the study. The animals were divided into two groups by random sampling, including 62 animals in the CSFA diet group and 44 animals in the basal ration (control; C) group. The modified Ovsynch protocol was used as the synchronization protocol. Pre-ovulatory follicle and uterine tension values were not significantly different between the CSFA and C groups (p>0.05). The rates of both the standing reflex and cervical mucus were greater in the CSFA group than in C (p<0.05). Conception rates on the 30th-35th and 60th-65th days were greater in the CSFA diet group than in C (p<0.05). Consequently, in this study, a positive correlation was identified between the appearance of estrus signs at the time of TAI application and conception outcomes. It was determined that supplementing 800 g/day CSFA to the rations of Simmental cattle with low rates of estrus signs in the transition and early lactation periods increased estrus signs and conception rates.Öğe Thymoquinone improves motility, plasma membrane integrity and DNA integrity of frozen-thawed ram semen(Wiley, 2022) İnanc, Muhammed Enes; Güngör, Şükrü; Avdatek, Fatih; Yeni, Deniz; Gülhan, Mehmet Fuat; Olgaç, Kemal Tuna; Denk, Barış; Taşdemir, UmutThe aim of this study was to determine the effects of thymoquinone (TQ), which is the most essential active compound of Nigella sativa, on the spermatological parameters of ram semen during cryopreservation. Ejaculates were collected from five Sonmez rams using an artificial vagina and extended with Tris-based extender not containing TQ (control, 0 mu g/ml TQ) and containing 10, 25, 50 and 100 mu g/ml TQ. The extended semen samples were equilibrated in a + 4 degrees C cold cabinet for 2 h. After 2 h, the samples were loaded into 0.25 ml French straws. The straws were frozen by liquid nitrogen vapour and stored in a liquid nitrogen container (-196 degrees C). The frozen straws were thawed in a water bath (37 degrees C for 30 s) and evaluated in terms of motility characteristics, plasma membrane and acrosome integrity, mitochondrial reactive oxygen species levels, lipid peroxidation levels, DNA damage and biochemical alterations (oxidative stress index, malondialdehyde and glutathione). TQ100 had higher total motility (53.59 +/- 3.01) and progressive motility (19.84 +/- 1.44; not significantly different from TQ25 and TQ50) compared to the control and TQ10 (p < 0.05). According to the results of the analyses on motility characteristics, there were significant differences between the groups in terms of curvilinear velocity (VCL), amplitude of lateral head displacement (ALH) and linearity (LIN; p < 0.05). The highest DNA damage was detected in the control group (p < 0.05). TQ50 had higher plasma membrane and acrosome integrity (59.56 +/- 5.92) compared to the control and TQ25 (p < 0.05) but not significantly different from TQ10 and TQ100. The lowest mitochondrial reactive oxygen species levels were detected in TQ50 and TQ100 (p < 0.05). There were no significant differences among the groups in terms of their oxidative stress index, lipid peroxidation, malondialdehyde and glutathione levels (p > 0.05). According to the results, it could be concluded that supplementing 50 or 100 mu g/ml TQ to Tris extenders that were used for ram semen cryopreservation showed a positive effect on motility, plasma membrane integrity and acrosome integrity, and it reduced DNA damage and mitochondrial reactive oxygen species levels.
