Supplementation of rosmarinic acid has reduced oxidative stress on bull spermatozoa followİng the freeze thawing process
[ X ]
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Cryo-Letters
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
BACKGROUND: Cryopreservation has a side effect on the motility, chromatin integrity and viability of sperm cells. OBJECTIVE: The present study investigated the effects of supplementation with rosmarinic acid (RA) Tris extender on sperm quality parameters, plasma and acrosome membrane damage, antioxidant enzyme activity and chromatin integrity following the freeze thawing process on bull spermatozoa. MATERIALS AND METHODS: Ejaculates were split into five aliquots and diluted to a final concentration of 15x106 spermatozoa/ml with the Tris extender containing RA (25, 50, 100 and 200 µg/ml) and (control) and then frozen at a controlled rate. RESULT: Treatments did not give better results on the percentages of sperm progressive, total motility and sperm motion characters (P>0.05); however, RA25 and RA50 exhibited favourable chromatin integrity. In conclusion, RA25 and RA50 increased total antioxidant activity. As a consequence, the amount of MDA and chromatin damage were reduced in sperm cells. © CryoLetters.
Açıklama
PubMed ID: 29734425
Anahtar Kelimeler
Antioxidant, Bull Semen Cryopreservation, Oxidative Stress, Rosmarinic Acid
Kaynak
Cryo-Letters
WoS Q Değeri
N/A
Scopus Q Değeri
Q3
Cilt
39
Sayı
2
