Cryoprotective effect of vaccenic acid supplemented in bull semen extender
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Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Scientific and Technological Research Council of Turkey
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The aim of this study was to investigate whether vaccenic acid (VA) added to TRIS semen extender has an effect on freezing bull semen. The semen was collected at intervals of three days through an artificial vagina then pooled. The pooled ejaculates were divided into five components as the control (C; no supplement) and VA (25, 50, 100, and 200 mu g / mL) groups then extended with the TRIS-based medium. The samples were left in a cold air cabinet for cooling process (4 degrees C), then they were equilibrated for 4 h. Afterward, the samples in the groups were frozen by using a programmable freezer. The greatest progressive and total motility results were obtained in the VA25 group (p < 0.001). Besides, the total abnormality and chromatin damage was determined to be on the lowest level in the VA25 group (p < 0.05). Except for VA200, the glutathione level was greater in the treatment groups in comparison to the control group (p < 0.05), while the lowest malondialdehyde was determined in VA25. In conclusion, VA supplemented with TRIS-based extender at the concentration of 25 mu g / mL improves the progressive and total motility properties of spermatozoa, reduces the abnormal spermatozoa rate, and has a positive effect on chromatin integrity.
Açıklama
Anahtar Kelimeler
Antioxidant, Bull Semen, Cryopreservation, Chromatin Integrity, Oxidative Stress
Kaynak
Turkish Journal of Veterinary and Animal Sciences
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
46
Sayı
1
