Investigation of changes in spermatozoon characteristics, chromatin structure, and antioxidant/oxidant parameters after freeze-thawing of hesperidin (Vitamin P) doses added to ram semen

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Tarih

2022

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

MDPI

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

We conducted this study to determine the potential cryopreservative effects of different hesperidin (vitamin P; H) doses on ram semen after freeze-thawing. Semen samples were obtained from Sonmez rams using an artificial vagina. The samples were divided into six groups: control, 10, 50, 100, 250, and 500 mu g/mL H (C, H10, H50, H100, H250, and H500, respectively). At the end of the study, sperm motility and kinetic parameters, acrosome integrity (AI), mitochondrial membrane potential (MMP), viability, lipid peroxidation levels (LPL), chromatin damage, oxidant parameters, and antioxidant parameters were assayed. None of the doses of H added to the semen extender showed any enhancing effects on progressive motility compared to C (p > 0.05). In fact, H500 had negative effects (p < 0.05). Moreover, AI was the highest at the H10 dose, while LPL values were the lowest at the same dose (p < 0.05). The doses of H10 and H50 added to the Tris extender medium showed positive effects on sperm cell chromatin damage. Consequently, we can say that H doses used in this study are not effective on semen progressive motility, but the H10 dose is effective on AI and chromatin damage by reducing LPL.

Açıklama

Anahtar Kelimeler

Semen, Hesperidin, Cryopreservation, Oxidative Stress, Antioxidantram

Kaynak

Life-Basel

WoS Q Değeri

Q2

Scopus Q Değeri

Cilt

12

Sayı

11

Künye