Investigation of changes in spermatozoon characteristics, chromatin structure, and antioxidant/oxidant parameters after freeze-thawing of hesperidin (Vitamin P) doses added to ram semen

dc.authorid0000-0002-9105-5677
dc.authorid0000-0003-3460-522X
dc.authorid0000-0003-2345-8826
dc.authorid0000-0003-4838-1597
dc.authorid0000-0001-9216-7059
dc.authorid0000-0001-6954-6309
dc.authorid0000-0002-7586-0895
dc.authorid0000-0003-2827-1286
dc.contributor.authorYeni, Deniz
dc.contributor.authorGüngor, Şükrü
dc.contributor.authorAvdatek, Fatih
dc.contributor.authorGülhan, Mehmet Fuat
dc.contributor.authorOlgaç, Kemal Tuna
dc.contributor.authorİnanç, Muhammed Enes
dc.contributor.authorDenk, Barış
dc.contributor.authorTaşdemir, Umut
dc.date.accessioned2023-01-20T06:20:34Z
dc.date.available2023-01-20T06:20:34Z
dc.date.issued2022
dc.departmentVeteriner Fakültesi
dc.description.abstractWe conducted this study to determine the potential cryopreservative effects of different hesperidin (vitamin P; H) doses on ram semen after freeze-thawing. Semen samples were obtained from Sonmez rams using an artificial vagina. The samples were divided into six groups: control, 10, 50, 100, 250, and 500 mu g/mL H (C, H10, H50, H100, H250, and H500, respectively). At the end of the study, sperm motility and kinetic parameters, acrosome integrity (AI), mitochondrial membrane potential (MMP), viability, lipid peroxidation levels (LPL), chromatin damage, oxidant parameters, and antioxidant parameters were assayed. None of the doses of H added to the semen extender showed any enhancing effects on progressive motility compared to C (p > 0.05). In fact, H500 had negative effects (p < 0.05). Moreover, AI was the highest at the H10 dose, while LPL values were the lowest at the same dose (p < 0.05). The doses of H10 and H50 added to the Tris extender medium showed positive effects on sperm cell chromatin damage. Consequently, we can say that H doses used in this study are not effective on semen progressive motility, but the H10 dose is effective on AI and chromatin damage by reducing LPL.
dc.identifier.doi10.3390/life12111780
dc.identifier.issn2075-1729
dc.identifier.issue11en_US
dc.identifier.pmid36362935
dc.identifier.urihttps:/dx.doi.org/10.3390/life12111780
dc.identifier.urihttps://hdl.handle.net/20.500.12451/9989
dc.identifier.volume12en_US
dc.identifier.wosWOS:000895117300001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMDPI
dc.relation.ispartofLife-Basel
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSemen
dc.subjectHesperidin
dc.subjectCryopreservation
dc.subjectOxidative Stress
dc.subjectAntioxidantram
dc.titleInvestigation of changes in spermatozoon characteristics, chromatin structure, and antioxidant/oxidant parameters after freeze-thawing of hesperidin (Vitamin P) doses added to ram semen
dc.typeArticle

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