A new application of inorganic sorbent for biomolecules: IMAC practice of Fe3+-nano flowers for DNA separation
| dc.authorid | 0000-0002-1414-4465 | |
| dc.contributor.author | Serinbaş, Ayça | |
| dc.contributor.author | Önal, Burcu | |
| dc.contributor.author | Acet, Ömür | |
| dc.contributor.author | Özdemir, Nalan | |
| dc.contributor.author | Dzmitruk, Volha | |
| dc.contributor.author | Halets-Bui, Inessa V. | |
| dc.contributor.author | Shcharbin, Dzmitry G. | |
| dc.contributor.author | Odabaşı, Mehmet | |
| dc.date.accessioned | 2020-06-08T10:47:09Z | |
| dc.date.available | 2020-06-08T10:47:09Z | |
| dc.date.issued | 2020 | |
| dc.department | Sabire Yazıcı Fen Edebiyat Fakültesi | |
| dc.description.abstract | Selection of purification method and type of adsorbent has high significance for separation of a biomolecule like deoxyribonucleic acid (DNA). Nanoflowers are a newly improved class of adsorbent. Due to showing very structural similarity to plant flowers, they are named as nanoflowers. Herein, after synthesize of copper phosphate three hydrate nanoflowers [(Cu3(PO4)2.3H2O), CP-NFs], Fe3+ ions were attached to their surfaces. Obtained Fe3+-CP-NFs, before investigation of some adsorption parameters for DNA, they were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). Some attained data from the results of adsorption experiments as follows: While maximum DNA adsorption on Fe3+-CP-NFs was found as an excellent value of 845.8 mg/g, nanoflowers without Fe3+ ions adsorbed DNA as only 25.3 mg/g. Optimum media conditions for DNA adsorption were observed at pH 7 and 25 °C with an initial concentration of 1.5 mg/mL DNA. | |
| dc.identifier.doi | 10.1016/j.msec.2020.111020 | |
| dc.identifier.endpage | - | en_US |
| dc.identifier.issn | 0928-4931 | |
| dc.identifier.issue | - | en_US |
| dc.identifier.pmid | 32487418 | |
| dc.identifier.scopusquality | N/A | |
| dc.identifier.startpage | - | en_US |
| dc.identifier.uri | https://dx.doi.org/10.1016/j.msec.2020.111020 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12451/7549 | |
| dc.identifier.volume | 113 | en_US |
| dc.identifier.wos | WOS:000537712500036 | |
| dc.identifier.wosquality | Q1 | |
| dc.indekslendigikaynak | Web of Science | |
| dc.indekslendigikaynak | Scopus | |
| dc.indekslendigikaynak | PubMed | |
| dc.language.iso | en | |
| dc.publisher | Elsevier Ltd | |
| dc.relation.ispartof | Materials Science and Engineering C | |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | |
| dc.rights | info:eu-repo/semantics/embargoedAccess | |
| dc.subject | Copper Phosphate Nanoflowers | |
| dc.subject | IMAC | |
| dc.subject | DNA Separation | |
| dc.title | A new application of inorganic sorbent for biomolecules: IMAC practice of Fe3+-nano flowers for DNA separation | |
| dc.type | Article |
