Preparation of a new chromatographic media and assessment of some kinetic and interaction parameters for lysozyme

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dc.contributor.authorAcet, Ömür
dc.contributor.authorÖnal, Burcu
dc.contributor.authorSanz, Raul
dc.contributor.authorSanz-Perez, Eloy S.
dc.contributor.authorErdönmez, Demet
dc.contributor.authorOdabaşı, Mehmet
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:16:28Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:16:28Z
dc.date.issued2019
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractSilica based SBA-15 nanoparticles functionalized with aminopropyl-trimethoxysilane (AP) groups were prepared, and then Cu2+ ions were decorated onto them via AP (Cu+2-SBA-15), and calculated by ICP-OES as 137.9 mg/g nanoparticles. The characterizations of SBA-15 and SBA-15-AP were examined by XRD, FTIR and BET analysis. Prepared Cu+2-SBA-15 nanoparticles were embedded into cryogel column (Cu+2-SBA-15 NEC) photographed by SEM, and tried to investigate some adsorption parameters against to lysozyme selected as model protein. Lysozyme adsorption studies were conducted in a continuous system. The maximum adsorption capacity (1275.2 mg/g particles) of Cu+2-SBA-15 nanoparticles was obtained at phosphate buffer of pH 7.0 with initial lysozyme concentration of 1 mg/mL. According to examined Langmuir and Freundlich adsorption isotherms, Langmuir isotherm was fitted the best to this study. Composite cryogels were also used for 30 times in adsorption-desorption experiments. Purification capability of matrix was expressed as 88.5% with 913 purification yield from hen egg white (HEW) by means of SDS-PAGE. (C) 2018 Elsevier B.V. All rights reserved.
dc.identifier.doi10.1016/j.molliq.2018.12.037
dc.identifier.endpage487en_US
dc.identifier.issn0167-7322
dc.identifier.issn1873-3166
dc.identifier.scopusqualityQ1
dc.identifier.startpage480en_US
dc.identifier.urihttps://doi.org/10.1016/j.molliq.2018.12.037
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4600
dc.identifier.volume276en_US
dc.identifier.wosWOS:000459528600053
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofJournal of Molecular Liquids
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectSBA-15 Nanoparticles
dc.subjectEmbedding Cryogels
dc.subjectIMAC
dc.subjectMonolith
dc.subjectLysozyme Adsorption
dc.titlePreparation of a new chromatographic media and assessment of some kinetic and interaction parameters for lysozyme
dc.typeArticle

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