A novel matrix for hydrophobic interaction chromatography and its application in lysozyme adsorption

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dc.contributor.authorGedikli, Mehmet
dc.contributor.authorCeylan, Şeyda
dc.contributor.authorErzengin, Mahmut
dc.contributor.authorOdabaşı, Mehmet
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T08:22:32Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T08:22:32Z
dc.date.issued2014
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.descriptionPubMed ID: 25298972
dc.description.abstractA novel 1-naphthylamine (NA) coupled poly(2-hydroxyethyl methacrylate-co-N-methacryloyl-(L)-histidine methyl ester) [NA-PHEMAH] supermacroporous monolithic hydrophobic cryogel was prepared via covalent coupling of NA to PHEMAH for adsorption of lysozyme from aqueous solution. Firstly, PHEMAH monolithic cryogel was prepared by radical cryocopolymerization of HEMA with MAH as a functional comonomer and N,N'-methylene-bisacrylamide (MBAAm) as a crosslinker directly in a plastic syringe, and then NA molecules were covalently attached to the imidazole rings of MAH groups of the polymeric structure. The prepared, NA-PHEMAH, supermacroporous monolithic hydrophobic cryogel was characterized by scanning electron microscopy (SEM). The effects of initial lysozyme concentration, pH, salt type, temperature and flow rate on the adsorption efficiency of monolithic hydrophobic cryogel were studied in a column system. The maximum amount of lysozyme adsorption from aqueous solution in phosphate buffer was 86.1 mg/g polymer at pH 8.0 with a flow rate of 1 mL/min. It was observed that lysozyme could be repeatedly adsorbed and desorbed with the NA-PHEMAH monolithic hydrophobic cryogel without significant loss of the adsorption capacity.
dc.identifier.endpage737en_US
dc.identifier.issn0001-527X
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ3
dc.identifier.startpage731en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12451/2585
dc.identifier.volume61en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherPolskie Towarzystwo Biochemiczne
dc.relation.ispartofActa Biochimica Polonica
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject1-Naphthylamine
dc.subjectCryogel
dc.subjectHydrophobic Interaction Chromatography
dc.subjectLysozyme
dc.subjectPHEMA
dc.subjectSEM
dc.titleA novel matrix for hydrophobic interaction chromatography and its application in lysozyme adsorption
dc.typeArticle

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