Cryopreservation effects on ram sperm ultrastructure

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dc.authorid0000-0001-9502-1178
dc.contributor.authorKeskin, Nazan
dc.contributor.authorErdoğan, Cennet
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorÖztürk, Ali Erdem
dc.contributor.authorBodu, Mustafa
dc.contributor.authorİli, Pınar
dc.contributor.authorBaşpınar, Nuri
dc.contributor.authorDursun, Şükrü
dc.date.accessioned2021-06-16T10:32:29Z
dc.date.available2021-06-16T10:32:29Z
dc.date.issued2020
dc.departmentMühendislik Fakültesi
dc.description*Dursun, Şükrü ( Aksaray, Yazar )
dc.description.abstractCryoprotectants are known to have protective effects against cryodamage to spermatozoa. In this study, the cryoprotective effects of two cryoprotectants (glycerol, ethylene glycol) and cryoprotectants/trehalose combinations on frozen-thawed ram spermatozoa were investigated at the ultrastructural level. For this purpose, ejaculates collected from Konya Merino rams were pooled and diluted with a tris-based extender containing additives, including 5% glycerol, 3% glycerol +60 mM trehalose, 1.5% glycerol +100 mM trehalose, 5% ethylene glycol, 3% ethylene glycol +60 mM trehalose, and 1.5% ethylene glycol +100 mM trehalose. They were all cooled to 5°C and then frozen in 0.25 mL French straws in liquid nitrogen. The samples were thawed at 37°C and centrifuged to remove the diluents. Then, they were processed using a scanning transmission electron microscope. In the statistical analysis, the number of ultrastructurally cryodamaged and intact spermatozoa were counted in longitudinal and transverse ultrathin sections in all groups by electron microscopic examination. The amount of intact spermatozoa in the groups containing 5% ethylene glycol and 1.5% ethylene glycol +100 mM trehalose was found to be higher than other groups (p < 0.05). As a result, it was suggested that the groups of 5% ethylene glycol and 1.5% ethylene glycol +100 mM trehalose provided the highest protection for the ultrastructural morphology of frozen-thawed Konya Merino ram spermatozoa among the groups.
dc.identifier.doi10.1089/bio.2020.0056
dc.identifier.endpage448en_US
dc.identifier.issn1947-5535
dc.identifier.issue5en_US
dc.identifier.pmid32816526
dc.identifier.scopusqualityQ2
dc.identifier.startpage441en_US
dc.identifier.urihttps:/dx.doi.org/10.1089/bio.2020.0056
dc.identifier.urihttps://hdl.handle.net/20.500.12451/8119
dc.identifier.volume18en_US
dc.identifier.wosWOS:000563395000001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMary Ann Liebert Inc.
dc.relation.ispartofBiopreservation and Biobanking
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectCryopreservation
dc.subjectElectron Microscopy
dc.subjectEthylene Glycol
dc.subjectGlycerol
dc.subjectKonya Merino Ram Spermatozoa
dc.subjectTrehalose
dc.titleCryopreservation effects on ram sperm ultrastructure
dc.typeArticle

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