Novel adsorbent for DNA adsorption: Fe (3+)-attached sporopollenin particles embedded composite cryogels

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dc.contributor.authorCeylan, Seyda
dc.contributor.authorOdabaşı, Mehmet
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:28:26Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:28:26Z
dc.date.issued2013
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractThe aim of this study is to prepare supermacroporous cryogels embedded with Fe3+-attached sporopollenin particles (Fe3+-ASPs) having large surface area for high DNA adsorption capacity. Supermacroporous poly(2-hydroxyethyl methacrylate) (PHEMA)-based monolithic cryogel column embedded with Fe3+-ASPs was prepared by radical cryo-copolymerization of 2-hydroxyethyl methacrylate (HEMA) with N, N'-methylenebis-acrylamide (MBAAm) as cross-linker directly in a plastic syringe for DNA adsorption studies. Firstly, Fe3+ ions were attached to the sporopollenin particles (SPs), then the supermacroporous PHEMA cryogel with embedded Fe3+-ASPs was produced by free radical polymerization using N, N, N', N'-Tetramethylethylenediamine (TEMED) and ammonium persulfate (APS) as initiator/activator pair in an ice bath. Optimum conditions of adsorption experiments were performed at pH 6.0 (0.02 M Tris buff er containing 0.2 M NaCl), with flow rate of 0.5 mL/min, and at 5 degrees C. The maximum amount of DNA adsorption from aqueous solution was very high (109 mg/g SPs) with initial concentration of 3 mg/mL. It was observed that DNA could be repeatedly adsorbed and desorbed with this composite cryogel without signifi cant loss of adsorption capacity. As a result, higher amounts of DNA adsorbed these composite cryogels are expected to be good candidate for achieving higher removal of anti-DNA antibodies from systemic lupus erythematosus (SLE) patients plasma.
dc.identifier.doi10.3109/21691401.2012.759125
dc.identifier.endpage383en_US
dc.identifier.issn2169-1401
dc.identifier.issn2169-141X
dc.identifier.issue6en_US
dc.identifier.pmid23305206
dc.identifier.scopusqualityQ1
dc.identifier.startpage376en_US
dc.identifier.urihttps://doi.org/10.3109/21691401.2012.759125
dc.identifier.urihttps://hdl.handle.net/20.500.12451/6039
dc.identifier.volume41en_US
dc.identifier.wosWOS:000329843900004
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherInforma Healthcare
dc.relation.ispartofArtificial Celss Nanomedicine and Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAffinity Chromatography
dc.subjectDNA Adsorption
dc.subjectMetal Chelate
dc.subjectParticle Embedding
dc.subjectSporopollenin
dc.titleNovel adsorbent for DNA adsorption: Fe (3+)-attached sporopollenin particles embedded composite cryogels
dc.typeArticle

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