In vitro and in vivo effects of commercial and environmental microplastics on Unio delicatus

dc.authorid0000-0001-5910-2835
dc.authorid0000-0002-9072-543X
dc.authorid0000-0003-2841-0928
dc.authorid0000-0002-0385-3602
dc.authorid0000-0003-4049-6197
dc.authorid0000-0003-0431-9919
dc.authorid0000-0001-7551-4899
dc.authorid0000-0002-8448-8976
dc.authorid0000-0002-2800-6215
dc.authorid0000-0001-8462-415X
dc.contributor.authorYüce, Pınar Arslan
dc.contributor.authorGünal, Aysel Çağlan
dc.contributor.authorErkmen, Belda
dc.contributor.authorYurdakök Dikmen, Begüm
dc.contributor.authorÇağan, Ali Serhan
dc.contributor.authorÇırak, Tamer
dc.contributor.authorBaşaran Kankılıç, Gökben
dc.contributor.authorSeyfe, Melike
dc.contributor.authorFilazi, Ayhan
dc.contributor.authorTavşanoğlu, Ülkü Nihan
dc.date.accessioned2024-11-14T12:42:36Z
dc.date.available2024-11-14T12:42:36Z
dc.date.issued2024
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractMicroplastics (MPs) are ubiquitous pollutants in freshwater environments. In this study, freshwater mussels, Unio delicatus, were exposed to both environmental MPs (e-MP) and commercial MPs (c-MP) that include green fluorescent MP (gf-MP), polyethylene (c-PE) and polystyrene (c-PS) at environmental concentrations (5 mg/L and 50 mg/L) over duration of 7 and 30 days. According to in vivo experiment results, both e-MPs and c-MPs induced significant changes in the total hemocyte counts of mussels (p < 0.05). Exposure to high concentrations of e-MPs and c-MPs for 7 days led to decreased cellular glutathione levels in the mussels, while exposure to low concentrations of e-MPs and c-PS for 7 days resulted in increased advanced oxidation protein products (AOPP). Mussels exposed to high concentrations of e-MPs for 30 days exhibited decreases in both glutathione levels and AOPP values. Although no damage was observed in tissues other than gills and digestive gland, histopathological alterations were observed in these tissues following exposure to 50 mg/L c-MPs. Additionally, MPs were observed in the intestine tissues. In vitro experiments using the MTT assay showed no significant difference in cell viability between the MP-exposed group and the control group at tested concentrations, with no observed dose-response relationship (p > 0.05). Nevertheless, certain cells exhibited signs of cell death, such as disrupted cellular structures, condensed nuclei, and loss of cellular integrity. These observations were consistent with mechanical compression, indicating that physical contact with MPs may result in cell damage or death. These findings demonstrate that environmentally relevant concentrations of MPs have toxic effects on freshwater mussels and multiple parameters provide valuable insight for the evaluation of health risks of organisms.
dc.identifier.doi10.1007/s10646-024-02807-2
dc.identifier.issn0963-9292
dc.identifier.scopusqualityQ2
dc.identifier.urihttps:/dx.doi.org/10.1007/s10646-024-02807-2
dc.identifier.urihttps://hdl.handle.net/20.500.12451/12647
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofEcotoxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectAdvanced Oxidation Protein Products
dc.subjectCytotoxicity
dc.subjectFreshwater Mussel
dc.subjectGlutathione Level
dc.subjectHistopathology
dc.titleIn vitro and in vivo effects of commercial and environmental microplastics on Unio delicatus
dc.typeArticle

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