Red pine (Pinus brutia Ten) bark tree extract preserves sperm quality by reducing oxidative stress and preventing chromatin damage

dc.authorid0000-0002-9105-5677
dc.authorid0000-0003-2822-1625
dc.authorid0000-0003-4726-3900
dc.contributor.authorTaşdemir, Umut
dc.contributor.authorYeni, Deniz
dc.contributor.authorİnanç, Muhammed Enes
dc.contributor.authorAvdatek, Fatih
dc.contributor.authorÇil, Beste
dc.contributor.authorTürkmen, Ruhi
dc.date.accessioned2020-06-11T07:25:02Z
dc.date.available2020-06-11T07:25:02Z
dc.date.issued2020
dc.departmentTeknik Bilimler Meslek Yüksekokulu
dc.descriptionTaşdemir, Umut ( Aksaray, Yazar )
dc.description.abstractThis study aimed to investigate the effectiveness of using red pine bark tree extract (P; Pinus brutia Ten) as a TRIS extender in an attempt to prevent oxidative stress in bull spermatozoa after freezing. Semen specimens were obtained from Simmental bulls via an artificial vagina and pooled. They were separated into five specimens and diluted with Tris extender consisting of P (200, 100, 50 and 25 µg/ml) and P free (control; C) up to a final concentration of 16 × 106 per straw. All specimens were equilibrated for a period of 4 hr at a temperature of 4°C, following which they were filled in 0.25-ml French straws and frozen. Addition of P resulted in favourable tail length in comparison with C (p <.05). The lowest malondialdehyde levels and the highest glutathione levels were detected in all P groups (p <.05). Supplementation with P did not show advanced results in terms of total, progressive sperm motility and total abnormality in comparison with C (p >.05). In conclusion, it has been shown that although P added to a Tris extender does not have a positive effect on sperm motility, it prevents chromatin damage by reducing oxidative stress, in addition to reducing head abnormalities when used at the amount of 50 ?g/ml.
dc.identifier.doi10.1111/and.13603
dc.identifier.endpage-en_US
dc.identifier.issn0303-4569
dc.identifier.issue-en_US
dc.identifier.pmid32294298
dc.identifier.scopusqualityQ1
dc.identifier.startpage-en_US
dc.identifier.urihttps://dx.doi.org/10.1111/and.13603
dc.identifier.urihttps://hdl.handle.net/20.500.12451/7577
dc.identifier.volume-en_US
dc.identifier.wosWOS:000525769500001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherBlackwell Publishing Ltd
dc.relation.ispartofAndrologia
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectAntioxidant
dc.subjectChromatin Damage
dc.subjectCryopreservation
dc.subjectOxidative Stress
dc.subjectSpermatozoa
dc.titleRed pine (Pinus brutia Ten) bark tree extract preserves sperm quality by reducing oxidative stress and preventing chromatin damage
dc.typeArticle

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