Synthesis of hydrophobic nanoparticles for real-time lysozyme detection using surface plasmon resonance sensor

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dc.authoridyilmaz, erkut -- 0000-0002-1217-5225;
dc.contributor.authorSaylan, Yeşeren
dc.contributor.authorYılmaz, Fatma
dc.contributor.authorDerazshamshir, Ali
dc.contributor.authorYılmaz, Erkut
dc.contributor.authorDenizli, Adil
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:27:57Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:27:57Z
dc.date.issued2017
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractDiagnostic biomarkers such as proteins and enzymes are generally hard to detect because of the low abundance in biological fluids. To solve this problem, the advantages of surface plasmon resonance (SPR) and nanomaterial technologies have been combined. The SPR sensors are easy to prepare, no requirement of labelling and can be detected in real time. In addition, they have high specificity and sensitivity with low cost. The nanomaterials have also crucial functions such as efficiency improvement, selectivity, and sensitivity of the detection systems. In this report, an SPR-based sensor is developed to detect lysozyme with hydrophobic poly (N-methacryloyl-(L)-phenylalanine) (PMAPA) nanoparticles. The SPR sensor was first characterized by attenuated total reflection-Fourier transform infrared, atomic force microscope, and water contact angle measurements and performed with aqueous lysozyme solutions. Various concentrations of lysozyme solution were used to calculate kinetic and affinity coefficients. The equilibrium and adsorption isotherm models of interactions between lysozyme solutions and SPR sensor were determined and the maximum reflection, association, and dissociation constants were calculated by Langmuir model as 4.87, 0.019nM(-1), and 54nM, respectively. The selectivity studies of SPR sensor were investigated with competitive agents, hemoglobin, and myoglobin. Also, the SPR sensor was used four times in adsorption/desorption/recovery cycles and results showed that, the combination of optical SPR sensor with hydrophobic ionizable PMAPA nanoparticles in one mode enabled the detection of lysozyme molecule with high accuracy, good sensivity, real-time, label-free, and a low-detection limit of 0.66nM from lysozyme solutions. Lysozyme detection in a real sample was performed by using chicken egg white to evaluate interfering molecules present in the medium.
dc.identifier.doi10.1002/jmr.2631
dc.identifier.issn0952-3499
dc.identifier.issn1099-1352
dc.identifier.issue9en_US
dc.identifier.pmid28322473
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.1002/jmr.2631
dc.identifier.urihttps://hdl.handle.net/20.500.12451/5961
dc.identifier.volume30en_US
dc.identifier.wosWOS:000407478900004
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Molecular Recognition
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectHydrophobic Nanoparticle
dc.subjectlysozyme
dc.subjectSensor
dc.subjectSurface Plasmon Resonance
dc.titleSynthesis of hydrophobic nanoparticles for real-time lysozyme detection using surface plasmon resonance sensor
dc.typeArticle

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