Magnetic dye-affinity beads for human serum albumin purification

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dc.contributor.authorOdabaşı, Mehmet
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:27:52Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:27:52Z
dc.date.issued2011
dc.departmentFen-Edebiyat Fakültesi
dc.description.abstractCibacron Blue F3GA was covalently attached onto magnetic poly(vinyl alcohol) (mPVAL) beads (100-150m in diameter) for human serum albumin (HSA) adsorption from human plasma. Despite low nonspecific adsorption of HSA on mPVAL beads, Cibacron Blue F3GA attachment significantly increased the HSA adsorption. The maximum HSA adsorption was observed at pH 5.0. Higher HSA adsorption was observed from human plasma. Desorption of HSA from mPVAL beads was achieved by medium containing 1.0M KSCN at pH 8.0. To test the efficiency of albumin adsorption from human serum, before and after albumin adsorption was demonstrated with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analyses. HSA molecules could be reversibly adsorbed and desorbed 10 times with the magnetic beads without noticeable loss in their HSA adsorption capacity.
dc.identifier.doi10.1080/10826068.2010.540606
dc.identifier.endpage304en_US
dc.identifier.issn1082-6068
dc.identifier.issue3en_US
dc.identifier.pmid21660868
dc.identifier.scopusqualityQ2
dc.identifier.startpage287en_US
dc.identifier.urihttps://doi.org/10.1080/10826068.2010.540606
dc.identifier.urihttps://hdl.handle.net/20.500.12451/5944
dc.identifier.volume41en_US
dc.identifier.wosWOS:000291526400007
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTAYLOR & FRANCIS INC
dc.relation.ispartofPREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAffinity Beads
dc.subjectAlbumin Adsorption
dc.subjectCibacron Blue F3GA
dc.subjectDye Ligands
dc.subjectMagnetic Beads
dc.subjectPVAL
dc.titleMagnetic dye-affinity beads for human serum albumin purification
dc.typeArticle

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