Microbial community and ovine host response varies with early and late stages of Haemonchus contortus infection

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dc.authoridDINCEL, Gungor Cagdas -- 0000-0002-6985-3197
dc.contributor.authorEl-Ashram, Saeed
dc.contributor.authorAl Nasr, İbrahim
dc.contributor.authorAbouhajer, Fathi
dc.contributor.authorEl-Kemary, Maged
dc.contributor.authorHuang, Guangping
dc.contributor.authorDinçel, Güngör Ça?daş
dc.contributor.authorMehmood, Rashid
dc.contributor.authorHu, Min
dc.contributor.authorSuo, Xun
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:15:21Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:15:21Z
dc.date.issued2017
dc.departmentMeslek Yüksekokulu
dc.description.abstractThe interactions between gastric microbiota, ovine host, and Haemonchus contortus portray the ovine gastric environment as a complex ecosystem, where all factors play a pertinent role in fine-tuning each other and in haemeostasis. We delineated the impact of early and late Haemonchus infection on abomasal and ruminal microbial community, as well as the ovine host. Twelve, parasite-naive lambs were divided into four groups, 7 days post-infection (dpi) and time-matched uninfected-control groups; 50 dpi and time-matched uninfected control groups were used for the experiment. Six sheep were inoculated with 5000 H. contortus infective larvae and followed for 7 or 50 days with their corresponding uninfected-control ones. Ovine abomasal tissues were collected for histological analysis and gastric fluids were collected for PH value measurements, microbial community isolation and Illumina MiSeq platform and bioinformatic analysis. Our results showed that Haemonchus infection increased the abomasal gastric pH (P = 0.05) and resulted in necrotizing and inflammatory changes that were more severe during acute infection. Furthermore, infection increased the abomasal bacterial load and decreased the ruminal microbiome. A 7-day infection of sheep with H. contortus significantly altered approximately 98% and 94% of genera in the abomasal and ruminal bacterial profile, respectively (P = 0.040.05). However, the approximate altered genera 50 days after infection in the ovine abomasal and ruminal microbiome were about 62% and 69%, correspondingly (P = 0.04-0.05) with increase in some bacteria and decrease in others. Overall, these results indicate that Haemonchus infection plays a crucial role in shaping stomach microbial community composition, and diversity.
dc.description.sponsorshipNational Key Basic Research Program (973 program) of China [2015CB150300]; Foshan University, Foshan city, Guangdong province
dc.description.sponsorshipThis research was supported by the National Key Basic Research Program (973 program) of China (Grant No. 2015CB150300), and the Start-up Research Grant Program provided by Foshan University, Foshan city, Guangdong province for distinguished researchers. Additionally, the funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.identifier.doi10.1007/s11259-017-9698-5
dc.identifier.endpage277en_US
dc.identifier.issn0165-7380
dc.identifier.issn1573-7446
dc.identifier.issue4en_US
dc.identifier.pmid29098532
dc.identifier.scopusqualityQ2
dc.identifier.startpage263en_US
dc.identifier.urihttps://doi.org/10.1007/s11259-017-9698-5
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4359
dc.identifier.volume41en_US
dc.identifier.wosWOS:000415919400004
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSPRINGER
dc.relation.ispartofVETERINARY RESEARCH COMMUNICATIONS
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectHistological Analysis
dc.subjectHaemonchus Contortus
dc.subjectIllumina MiSeq Platform
dc.subjectBioinformatic Analysis
dc.subjectAbomasal and Ruminal Microbiome
dc.titleMicrobial community and ovine host response varies with early and late stages of Haemonchus contortus infection
dc.typeArticle

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