A rational approach for 3D recognition and removal of L-asparagine via molecularly imprinted membranes
| dc.contributor.author | Acet, Ömür | |
| dc.contributor.author | Ali Noma, Samir Abbas | |
| dc.contributor.author | Önal Acet, Burcu | |
| dc.contributor.author | Dikici, Emrah | |
| dc.contributor.author | Osman, Bilgen | |
| dc.contributor.author | Odabaşı, Mehmet | |
| dc.date.accessioned | 2023-02-23T06:26:57Z | |
| dc.date.available | 2023-02-23T06:26:57Z | |
| dc.date.issued | 2023 | |
| dc.department | Sabire Yazıcı Fen Edebiyat Fakültesi | |
| dc.description.abstract | In this study, a L-asparagine (L-Asn) imprinted membranes (L-Asn-MIPs) were synthesized via molecular imprinting for selective and efficient removal of L-Asn. The L-Asn-MIP membrane was prepared by using acrylamide (AAm) and hydroxyethyl methacrylate (HEMA) as a functional monomer and a comonomer, respectively. The membrane was characterized by scanning electron microscopy (SEM) and Fourier Transform infrared spectroscopy (FTIR). The L-Asn adsorption capacity of the membrane was investigated in detail. The maximum L-Asn adsorption capacity was determined as 408.2 mg/g at pH: 7.2, 24 °C. Determination of L-Asn binding behaviors of L-Asn-MIPs also shown with Scatchard analyses. The effect of pH on L-Asn adsorption onto the membrane and also the selectivity and reusability of the L-Asn-MIPs for L-Asn adsorption were determined through L-asparaginase (L-ASNase) enzyme activity measurements. The selectivity of the membrane was investigated by using two different ternary mixtures; L-glycine (L-Gly)/L-histidine (L-His)/L-Asn and L-tyrosin (L-Tyr)/L-cystein(L-Cys)/L-Asn. The obtained results showed that the L-Asn-MIP membranes have a high selectivity towards L-Asn. | |
| dc.identifier.doi | 10.1016/j.jpba.2023.115250 | |
| dc.identifier.issn | 0731-7085 | |
| dc.identifier.pmid | 36657352 | |
| dc.identifier.scopusquality | Q1 | |
| dc.identifier.uri | https:/dx.doi.org/10.1016/j.jpba.2023.115250 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12451/10273 | |
| dc.identifier.volume | 226 | en_US |
| dc.identifier.wos | WOS:000971451800001 | |
| dc.identifier.wosquality | Q2 | |
| dc.indekslendigikaynak | Web of Science | |
| dc.indekslendigikaynak | Scopus | |
| dc.indekslendigikaynak | PubMed | |
| dc.language.iso | en | |
| dc.publisher | Elsevier B.V. | |
| dc.relation.ispartof | Journal of Pharmaceutical and Biomedical Analysis | |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | |
| dc.rights | info:eu-repo/semantics/embargoedAccess | |
| dc.subject | Asparagine | |
| dc.subject | L-asparaginase Activity | |
| dc.subject | Molecular Imprinting | |
| dc.subject | Remov | |
| dc.title | A rational approach for 3D recognition and removal of L-asparagine via molecularly imprinted membranes | |
| dc.type | Article |
