Investigation of lysozyme adsorption performance of Cu2+-attached PHEMA beads embedded cryogel membranes

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dc.contributor.authorCömert, Şeyda Ceylan
dc.contributor.authorOdabaşı, Mehmet
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:26:40Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:26:40Z
dc.date.issued2014
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractFor the purification of large molecules, cryogels are an alternative stationary phase to particle based media. But, due to the drawbacks of cryogels (i.e., low surface area) and particle sorbents (i.e., pressure drop in fixed beds), in recent years, the use of hybrid cryogels has greatly increased. In this study, a novel hybrid cryogel column was synthesized for the purification of lysozyme from aqueous solutions and hen egg white (HEW). Firstly, poly(2-hydroxyethyl methacrylate) beads (2 mu m size) were prepared, and after iminodiacetic acid (IDA) immobilization, Cu2+ ions were attached via the IDA chelating groups. These arranged Cu2+-attached PHEMA beads (Cu2+-ABs) were located into PHEMA based cryogel in order to prepare Cu2+-AB5 embedded supermacroporous hybrid cryogel (Cu2+-ABsEHC) column. The specific surface area of the hybrid cryogel was found as 95 m(2)/g by using BET. The amount of IDA on beads was found as 875 mu mol IDA/g. It was approached to the optimum adsorption levels at initial lysozyme concentration of 4 mg/mL and pH 8.0 as 874.9 mg/g beads. The purity of lysozyme adsorbed from HEW was studied by SDS-PAGE with a purity of 86.4%. It is demonstrated that this column is a potential separation medium for purification of lysozyme from HEW. (C) 2013 Elsevier B.V. All rights reserved.
dc.identifier.doi10.1016/j.msec.2013.09.033
dc.identifier.endpage8en_US
dc.identifier.issn0928-4931
dc.identifier.issn1873-0191
dc.identifier.pmid24268226
dc.identifier.scopusqualityN/A
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1016/j.msec.2013.09.033
dc.identifier.urihttps://hdl.handle.net/20.500.12451/5671
dc.identifier.volume34en_US
dc.identifier.wosWOS:000330489500001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofMATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectProtein Adsorption
dc.subjectHybrid Cryogel
dc.subjectLysozyme
dc.subjectMetal Chelate
dc.subjectBeads Embedding
dc.titleInvestigation of lysozyme adsorption performance of Cu2+-attached PHEMA beads embedded cryogel membranes
dc.typeArticle

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