Can linoleic acid improve the quality of frozen thawed bull sperm?

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dc.contributor.authorBüyükleblebici, Serhat
dc.contributor.authorTaşdemir, Umut
dc.contributor.authorTuncer, Pürhan Barbaros
dc.contributor.authorDurmaz, Emre
dc.contributor.authorÖzgürtaş, Taner
dc.contributor.authorBüyükleblebici, Olga
dc.contributor.authorCoşkun, Erdem
dc.contributor.authorGürcan, İsmail Safa
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T08:22:22Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T08:22:22Z
dc.date.issued2014
dc.departmentVeteriner Fakültesi
dc.descriptionPubMed ID: 25583007
dc.description.abstractBACKGROUND: Cryopreservation is known to have a detrimental effect on the motility, viability and membrane integrity of sperm cells. OBJECTIVE: The aim of this study was to investigate the effect of various amount of linoleic acid supplementation to the Tris extender, on bull sperm parameters, DNA integrity and oxidative stress after freeze-thawing. METHODS: Ejaculates were split into five aliquots and extended to a final concentration of 18x10 6 spermatozoa/ml with the base extender containing different doses of linoleic acid 0.125 ml, (L125); 0.250 ml (L250); 0.5 ml (L500), 1 ml (L1000) and no additive (control; L0). The extended samples were equilibrated slowly to 4°C for 4 h and then froze using a digital freezing machine. Frozen straws were thawed individually in water bath at 37°C for 30 s to analyse progressive motility and sperm motion characteristics as well as membrane integrity. Biochemical assays were performed in a spectrophotometer using commercial kits. DNA damage was evaluated by Comet Assay. RESULT: The addition of various linoleic acid did not improve the sperm subjective, CASA and progressive motilities, sperm motility characteristics and DNA integrity (P>0.05). L500 exhibited the greatest values for membrane integrity than that of the other groups (P<0.001). All supplementation groups led to lower percentages of tail abnormalities in comparison to the control (P<0.001). L500 and L1000 significantly decreased total abnormalities. In conclusion, our findings showed that L500 linoleic acid supplementation in semen extender was of great beneficial effect on frozen-thawed bull semen in terms of morphology and plasma membrane integrity. © CryoLetters.
dc.identifier.endpage481en_US
dc.identifier.issn0143-2044
dc.identifier.issue6en_US
dc.identifier.scopusqualityQ3
dc.identifier.startpage473en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12451/2542
dc.identifier.volume35en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherCryo-Letters
dc.relation.ispartofCryo-Letters
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBull Sperm
dc.subjectCryopreservation
dc.subjectDNA Integrity
dc.subjectLinoleic Acid
dc.subjectOxidative Stress
dc.titleCan linoleic acid improve the quality of frozen thawed bull sperm?
dc.typeArticle

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