Circulating miRNAs in maternal plasma as potential biomarkers of early pregnancy in sheep

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dc.contributor.authorHitit, Mustafa
dc.contributor.authorKöse, Mehmet
dc.contributor.authorKaya, Mehmet Salih
dc.contributor.authorKırbaş, Mesut
dc.contributor.authorDursun, Şükrü
dc.contributor.authorAlak, İlyas
dc.contributor.authorAtlı, Mehmet Osman
dc.date.accessioned2022-10-04T05:57:15Z
dc.date.available2022-10-04T05:57:15Z
dc.date.issued2022
dc.departmentVeteriner Fakültesi
dc.description.abstractMicroRNA (miRNA) plays an important role in the control of gene expression and is implied in many biological functions, including embryo implantation and development. The aim was to assess plasma miRNA profiles during the peri-implantation and ascertain potential candidate miRNA markers for early pregnancy diagnosis in ovine plasma. The plasma samples were obtained from a total of 24 ewes on days 12 (pre-implantation; P12, n = 4), 16 (implantation; P16, n = 4) and 22 (post-implantation; P22, n = 4) after mating, and on their corresponding days of 12 (Pre-C; C12, n = 4), 16 (Imp-C; C16, n = 4) and 22 (Post-C; C22, n = 4) of the estrous cycle. The miRNA profiles in plasma were assessed by microarray technology. We detected the presence of 60 ovine-specific miRNAs in plasma samples. Of these miRNAs, 22 demonstrated a differential expression pattern, especially between the estrous cycle and early pregnancy, and targeted 521 genes. Two miRNAs (oar-miR-218a and oar-miR-1185-3p) were confirmed using RT-qPCR in the ovine plasma samples. Protein-protein interaction (PPI) network of target genes established six functional modules, of which modules 1 and 3 were enriched in the common GO terms, such as inflammatory response, defense response, and regulation of immune response. In contrast, module 2 was enriched in the developmental process involved in reproduction, embryo development, embryonic morphogenesis, and regulation of the developmental process. The results indicate that miRNAs profiles of plasma seemed to be modulated during the peri-implantation stage of pregnancy in ewes. Circulating miRNAs could be promising candidates for diagnosis in early ovine pregnancy.
dc.identifier.doi10.3389/fgene.2022.929477
dc.identifier.endpage-en_US
dc.identifier.issn1664-8021
dc.identifier.issue-en_US
dc.identifier.pmid36061213
dc.identifier.scopusqualityQ2
dc.identifier.startpage-en_US
dc.identifier.urihttps:/dx.doi.org/10.3389/fgene.2022.929477
dc.identifier.urihttps://hdl.handle.net/20.500.12451/9722
dc.identifier.volume13en_US
dc.identifier.wosWOS:000848520200001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherFrontiers Media SA
dc.relation.ispartofFrontiers in Genetics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectOvine
dc.subjectPlasma
dc.subjectmicroRNA
dc.subjectCirculating
dc.subjectEarly Pregnancy
dc.subjectExpression
dc.titleCirculating miRNAs in maternal plasma as potential biomarkers of early pregnancy in sheep
dc.typeArticle

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