Oxidative stress parameters of L929 cells cultured on plasma-modified PDLLA scaffolds

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dc.authoridVURAL, TAYFUN -- 0000-0001-9504-5532; SAGLAM, NECDET -- 0000-0002-5463-8355
dc.contributor.authorDemirbilek, Melike Erol
dc.contributor.authorDemirbilek, Murat
dc.contributor.authorKarahaliloğlu, Zeynep
dc.contributor.authorErdal, Ebru
dc.contributor.authorVural, Tayfun
dc.contributor.authorYalçın, Eda
dc.contributor.authorSağlam, Necdet
dc.contributor.authorDenkbaş, Emir Baki
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:27:09Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:27:09Z
dc.date.issued2011
dc.departmentFen-Edebiyat Fakültesi
dc.description.abstractOxidative stress may produce high level of reactive oxygen species (ROS) following cell exposure to endogenous and exogenous factors. Recent experiments implicate oxidative stress as playing an essential role in cytotoxicity of many materials. The aim of this study was to measure intracellular malondialdehyde (MDA), advanced oxidation protein product (AOPP) levels, and superoxide dismutase (SOD) activities of L929 fibroblasts cultured on PDLLA, polyethylene glycol (PEG), or ethylenediamine (EDA) grafted PDLLA by plasma polymerization method. Cell proliferation on these scaffolds was studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. The study showed that MDA, AOPP levels, and SOD activities in L929 fibroblast cells cultured on all scaffolds were significantly different compared to the control group and each other. The highest MDA (0.42 +/- 0.76 nmol/mg protein), AOPP (14.99 +/- 4.67 nmol/mg protein) levels, and SOD activities (7.49 +/- 3.74 U/mg protein) were observed in cells cultured on non-modified scaffolds; meanwhile, the most cell proliferation was obtained in EDA-modified scaffolds (MDA 0.15 +/- 0.14 nmol/mg protein, AOPP 13.12 +/- 3.86 nmol/mg protein, SOD 4.82 +/- 2.64 U/mg protein). According to our finding, EDA- or PEG-modified scaffolds are potentially useful as suitable biomaterials in tissue engineering.
dc.identifier.doi10.1007/s12010-011-9173-7
dc.identifier.endpage792en_US
dc.identifier.issn0273-2289
dc.identifier.issn1559-0291
dc.identifier.issue6en_US
dc.identifier.pmid21312003
dc.identifier.scopusqualityQ2
dc.identifier.startpage780en_US
dc.identifier.urihttps://doi.org/10.1007/s12010-011-9173-7
dc.identifier.urihttps://hdl.handle.net/20.500.12451/5801
dc.identifier.volume164en_US
dc.identifier.wosWOS:000291218200006
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherHumana Press
dc.relation.ispartofApplied Biochemistry and Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBiocompatibility
dc.subjectOxidative Stress
dc.subjectPDLLAscaffolds
dc.subjectL929fibroblasts
dc.subjectMDA
dc.subjectSOD
dc.subjectAOPP
dc.titleOxidative stress parameters of L929 cells cultured on plasma-modified PDLLA scaffolds
dc.typeArticle

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