Determination of some adsorption and kinetic parameters of alpha-amylase onto Cu+2-PHEMA beads embedded column

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dc.contributor.authorAcet, Ömür
dc.contributor.authorAksoy, Neşe Hayat
dc.contributor.authorErdönmez, Demet
dc.contributor.authorOdabaşı, Mehmet
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:17:15Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:17:15Z
dc.date.issued2018
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractIn order to investigate the biocatalytic properties of alpha-amylase on a composite cryogel matrix with immobilized metal affinity chromatography, Cu+2-attached poly(2-hydroxyethyl methacrylate) (Cu+2-PHEMA) beads, (2 mu m size) were synthesized, then composite cryogel column was prepared by composing beads and PHEMA cryogels. After the preparation of Cu+2-PHEMA beads embedded cryogel column (Cu+2-BEC), some experiments were tested. Accordingly, the highest adsorption capacity (676.8 mg/g particles) of cryogels was achieved at acetate buffer of pH 5.0 with initial alpha-amylase concentration of 4 mg/mL. Immobilized enzyme has more stable pH range, between 6 and 7.5 than, the free one. Immobilization also increased the optimal activity from 25 to temperature range of 25-35 degrees C. V-max and K-m of alpha-amylase were detected as 1.149 U/mg protein, and 11.6 x 10(-1) mM, respectively. alpha-Amylase was utilized 35 times repeatedly without losing the productivity.
dc.identifier.doi10.1080/21691401.2018.1501378
dc.identifier.endpageS545en_US
dc.identifier.issn2169-1401
dc.identifier.issn2169-141X
dc.identifier.pmid30299164
dc.identifier.scopusqualityQ1
dc.identifier.startpageS538en_US
dc.identifier.urihttps://doi.org/10.1080/21691401.2018.1501378
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4723
dc.identifier.volume46en_US
dc.identifier.wosWOS:000460141900051
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofArtificial Cells Nanomedicine and Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectProtein Adsorption
dc.subjectIMAC
dc.subjectAlpha-Amylase
dc.subjectComposite Cryogel
dc.subjectBead Embedding
dc.titleDetermination of some adsorption and kinetic parameters of alpha-amylase onto Cu+2-PHEMA beads embedded column
dc.typeArticle

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