Protein C recognition by ion-coordinated imprinted monolithic cryogels

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dc.authoridAsliyuce, Sevgi -- 0000-0003-0002-0856
dc.contributor.authorDemirci, Binnaz
dc.contributor.authorBereli, Nilay
dc.contributor.authorAslıyüce, Sevgi
dc.contributor.authorBaydemir, Gözde
dc.contributor.authorDenizli, Adil
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:28:45Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:28:45Z
dc.date.issued2017
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractThe protein C imprinted monolithic cryogel was synthesized using 2-hydroxyethyl methacrylate by redox cryo-polymerization method. The prepared monolithic cryogel was characterized by Fourier transform infrared spectroscopy, swelling test, surface area measurements, and scanning electron microscopy. The nonimprinted cryogel was prepared as well for control. Adsorption of protein C from aqueous solutions was investigated in a continuous mode and several parameters affecting adsorption performance were optimized. The maximum protein C adsorption amount was 30.4 mg/g. The selectivity studies were performed by monolithic column studies and fast protein liquid chromatography, using hemoglobin and human serum albumin as competing proteins. The relative selectivity coefficients were 2.37 and 8.89 for hemoglobin and human serum albumin, respectively. Reusability was tested for ten consecutive adsorption-desorption cycles, and no significant change in adsorption capacity was recorded. A pseudo-second-order model was suitable to interpret kinetic data, and the Langmuir model suited the adsorption isotherms well.
dc.identifier.doi10.1002/jssc.201600992
dc.identifier.endpage1620en_US
dc.identifier.issn1615-9306
dc.identifier.issn1615-9314
dc.identifier.issue7en_US
dc.identifier.pmid28195421
dc.identifier.scopusqualityQ2
dc.identifier.startpage1610en_US
dc.identifier.urihttps://doi.org/10.1002/jssc.201600992
dc.identifier.urihttps://hdl.handle.net/20.500.12451/6081
dc.identifier.volume40en_US
dc.identifier.wosWOS:000399782900021
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley-VCH
dc.relation.ispartofJournal of Separation Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCryogels
dc.subjectFast Protein Liquid Chromatography
dc.subjectMolecularly Imprinted Polymers
dc.subjectProtein Purification
dc.titleProtein C recognition by ion-coordinated imprinted monolithic cryogels
dc.typeArticle

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