The role of PRP in the healing of disc degeneration and the effect of local anesthetics on PRP

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dc.authorid0000-0001-5111-2051
dc.authorid0000-0003-0716-5695
dc.authorid0000-0003-4098-0907
dc.authorid0000-0002-9121-536X
dc.authorid0000-0003-1050-1645
dc.contributor.authorMert, Ahmet
dc.contributor.authorİkinci Keleş, Ayşe
dc.contributor.authorAydın, Murat
dc.contributor.authorErol, Hüseyin Serkan
dc.contributor.authorSönmez, Osman Fikret
dc.date.accessioned2025-07-18T06:07:26Z
dc.date.available2025-07-18T06:07:26Z
dc.date.issued2025
dc.departmentTıp Fakültesi
dc.description.abstractThis study aimed to investigate the regenerative effects of PRP on an experimental rat model of disc degeneration using histological and biochemical parameters. Additionally, we evaluated whether ropivacaine, a local anesthetic commonly used in clinical practice, affects the efficacy of PRP. Methods: Rats were randomly divided into five groups as control and treatment groups. Disc degeneration models were established using appropriate procedures. On the intervention day, PRP was prepared from whole blood collected from the rats. PRP, PRP + ropivacaine, or ropivacaine alone was administered at the appropriate doses and according to standardized protocols. Results: In the untreated disc degeneration groups, annulus fibrosus (AF) and nucleus pulposus (NP) boundaries were indistinct, disc morphology was disrupted, collagen structures in the AF were degenerated or irregular, and vacuolization, interstitial edema, and necrotic tissue remnants were observed in the NP region. In contrast, in groups treated with PRP and PRP + ropivacaine, a reduction in edema and vacuolization, disappearance of necrotic tissue, restoration of distinct NP and AF boundaries, and decreased atrophy and cellular clustering in NP cells were observed. Biochemical analysis showed that IL-6 and TNF-α levels were within normal ranges in the groups treated with PRP and PRP + ropivacaine, whereas these levels remained elevated in the untreated disc degeneration groups, indicating ongoing effects of degeneration. Conclusion: This study demonstrates the regenerative effects of PRP in disc degeneration through histological and biochemical parameters. Furthermore, the addition of ropivacaine to PRP did not exert any negative effects on PRP’s regenerative properties.
dc.identifier.doi10.3389/fbioe.2025.1613148
dc.identifier.issn22964185
dc.identifier.scopus105009589431
dc.identifier.urihttps://dx.doi.org/10.3389/fbioe.2025.1613148
dc.identifier.urihttps://hdl.handle.net/20.500.12451/13368
dc.identifier.volume13
dc.identifier.wosWOS:001521090900001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakScopus
dc.indekslendigikaynakWeb of Science
dc.institutionauthorİkinci Keleş, Ayşe
dc.institutionauthorid0000-0003-0716-5695
dc.language.isoen
dc.publisherFrontiers Media SA
dc.relation.ispartofFrontiers in Bioengineering and Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectInflammation
dc.subjectIntervertebral Disc Degeneration
dc.subjectLocal Anesthesia
dc.subjectPlatelet-rich Plasma
dc.subjectRat
dc.titleThe role of PRP in the healing of disc degeneration and the effect of local anesthetics on PRP
dc.typeArticle

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