Three-dimensional chitin rings from body segments of a pet diplopod species: Characterization and protein interaction studies

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dc.authoridSargin, Idris -- 0000-0003-3785-9575; Kaya, Murat -- 0000-0001-6954-2703; AKYUZ YILMAZ, Bahar -- 0000-0001-9760-9856
dc.contributor.authorKaya, Murat
dc.contributor.authorMulercikas, Povilas
dc.contributor.authorSargın, İdris
dc.contributor.authorKazlauskaite, Sonata
dc.contributor.authorBaublys, Vykintas
dc.contributor.authorAkyuz, Bahar
dc.contributor.authorBulut, Esra
dc.contributor.authorTubelyte, Vaida
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:28:48Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:28:48Z
dc.date.issued2016
dc.departmentFen-Edebiyat Fakültesi
dc.description.abstractPhysicochemical characterization of new chitin isolates can provide valuable insights into designing of biomimetic materials. Chitin isolates with a definite three-dimensional (3D) structure can exhibit characteristics that distinguish them from other chitin specimens that are in form of powder or flakes without a definite and uniform shape. Herein, 3D chitin rings were produced from body segments of a diplopod (Archispirostreptus gigas) inhabiting tropical regions. This organism is cultured easily and can reach 38 cm in length, which makes it a suitable source for isolation of chitin. The chitin rings were characterized via TGA, FT-IR, SEM and XRD analyses. Enzymatic digestion test with chitinase demonstrated that chitin isolates had high purity (digestion rate: 97.4%). The source organism had high chitin content; 21.02 +/- 2.23% on dry weight. Interactions of the chitin rings with bovine serum albumin (BSA) protein were studied under different conditions (pH: 4.0-8.0, chitin amount: 6-14 mg, contact time: 30-360 min, protein concentration: 0.2-1 mg/mL). The highest BSA adsorption was observed at pH 5.0 at 20 degrees C. The adsorption equilibrium data exhibited a better fit to Langmuir adsorption and the pseudo-first order kinetic models. The findings presented here can be useful for further studies aiming to develop biocompatible and nontoxic biomaterials.
dc.identifier.doi10.1016/j.msec.2016.06.046
dc.identifier.endpage722en_US
dc.identifier.issn0928-4931
dc.identifier.issn1873-0191
dc.identifier.pmid27524072
dc.identifier.scopusqualityN/A
dc.identifier.startpage716en_US
dc.identifier.urihttps://doi.org/10.1016/j.msec.2016.06.046
dc.identifier.urihttps://hdl.handle.net/20.500.12451/6088
dc.identifier.volume68en_US
dc.identifier.wosWOS:000382600000084
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofMATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subject3D Chitin
dc.subjectNanofibres
dc.subjectNanoporous
dc.subjectAlbumin
dc.titleThree-dimensional chitin rings from body segments of a pet diplopod species: Characterization and protein interaction studies
dc.typeArticle

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