Cellular localization and biological effects of 20nm-gold nanoparticles

Basit Öğe Kaydı
dc.authoridTan, Gamze -- 0000-0002-2755-1756
dc.contributor.authorTan, Gamze
dc.contributor.authorOnur, Mehmet Ali
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:29:58Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:29:58Z
dc.date.issued2018
dc.departmentSabire Yazıcı Fen-Edebiyat Fakültesi
dc.description.abstractGold nanoparticles (AuNPs) have recently emerged as prominent vehicles for many biomedical applications from sensing to delivery. The relevant literature contains conflicting data about the effects of AuNPs on living cells. The aim of present study is the synthesis and characterization of AuNPs at nanoscale, tracking their cellular localization and determining their effects on cell viability, migration and angiogenesis. Within this scope, 20 nm AuNPs were synthesized and characterized using various spectrometric techniques to determine their size, shape and surface properties such as charge and texture. Two main cell types including mouse fibroblast (L929) and human cervix adenocarcinoma (HeLa) were used in the study to compare the biological effects of colloidal gold on both non-cancer and cancer cells. AuNPs were allowed to interact with HeLa cells to determine their intracellular localization. AuNPs were mainly attached to the cell membrane/membranous compartments and to be captured in small amounts in cytoplasmic vacuoles or to be distributed freely in the cytosol. Scratch assay results showed that AuNPs reduced cancer cell migration especially at increasing concentrations. According to the chick chorioallantoic membrane assay, AuNPs exhibited strong anti-angiogenetic properties and can inhibit vascularization during angiogenesis. In addition, the MTT assay confirmed that AuNP-treated cells caused concentration dependent cytotoxic effects on both cell types. As a result, AuNPs not only have inhibitory effects on cancer cells, but also possess antiangiogenic activity, thus making them a multipotent agent for cancer therapy.
dc.description.sponsorshipHacettepe University Scientific Research Projects Coordination Unit [10BIYP02604003]
dc.description.sponsorshipContract grant sponsor: Hacettepe University Scientific Research Projects Coordination Unit; contract grant number: 10BIYP02604003
dc.identifier.doi10.1002/jbm.a.36373
dc.identifier.endpage1721en_US
dc.identifier.issn1549-3296
dc.identifier.issn1552-4965
dc.identifier.issue6en_US
dc.identifier.pmid29468810
dc.identifier.scopusqualityQ1
dc.identifier.startpage1708en_US
dc.identifier.urihttps://doi.org/10.1002/jbm.a.36373
dc.identifier.urihttps://hdl.handle.net/20.500.12451/6226
dc.identifier.volume106en_US
dc.identifier.wosWOS:000431004500024
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Biomedical Materials Research Part A
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectGold Nanoparticles
dc.subjectCytotoxicity
dc.subjectCellular Localization
dc.subjectCell Migration
dc.subjectAngiogenesis
dc.subjectChorioallantoic Membrane
dc.titleCellular localization and biological effects of 20nm-gold nanoparticles
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
tan-gamze-2018.pdf
Boyut:
1.11 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu