Efficient expression of recombinant human telomerase inhibitor 1 (hPinX1) in Pichia pastoris

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dc.authoridderyanur -- 0000-0002-9115-136X
dc.contributor.authorÜnver, Yağmur
dc.contributor.authorYıldız, Melike
dc.contributor.authorKılıç, Deryanur
dc.contributor.authorTaşkın, Mesut
dc.contributor.authorFırat, Abdulhadi
dc.contributor.authorAşkın, Hakan
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:17:37Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:17:37Z
dc.date.issued2018
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractPinX1 encoded by a remarkable tumor suppressor gene and located in human chromosome 8p23 is known as telomerase inhibitor. In recent years, this protein has been of interest as clinically tumor suppressor. Pichia pastoris expression system is preferred to produce heterologous proteins and is suitable for industrial and research purposes. In the present study, human PinX1 gene (hPinX1) was cloned in E. coli One Shot TOP10 cells and overexpressed in P. pastoris strain X-33 intracellularly, using a strong AOX (alcohol oxidase) promoter. The recombinant cells were grown in shaking flask. Induction time, methanol concentration and initial pH were optimized for obtaining high levels of hPinX1 protein production. Recombinant protein production was confirmed by Western blot analysis and the relative expression levels of rhPinX1 were quantified. According to Western blot analysis, molecular mass of produced hPinX1 was determined as 47.5kDa. At the end of optimization studies, the best fermentation conditions were determined as induction time 48h, methanol concentration 3% and initial culture pH 5.0. This process would be an applicable way for obtaining recombinant hPinX1 using P. pastoris expression system. This is the first report on recombinant production of hPinX1 in P. pastoris.
dc.identifier.doi10.1080/10826068.2018.1466160
dc.identifier.endpage540en_US
dc.identifier.issn1082-6068
dc.identifier.issn1532-2297
dc.identifier.issue6en_US
dc.identifier.pmid29958061
dc.identifier.scopusqualityQ2
dc.identifier.startpage535en_US
dc.identifier.urihttps://doi.org/10.1080/10826068.2018.1466160
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4773
dc.identifier.volume48en_US
dc.identifier.wosWOS:000451222800011
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Inc
dc.relation.ispartofPreparative Biochemistry & Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectExpression
dc.subjecthPinX1
dc.subjectOptimization
dc.subjectPichia Pastoris
dc.subjectProduction
dc.subjectYeast
dc.titleEfficient expression of recombinant human telomerase inhibitor 1 (hPinX1) in Pichia pastoris
dc.typeArticle

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