Comparison of the efficacies of Rhodococcus equi recombinant vaccine in mice

dc.contributor.authorSanioğlu Gölen, Gökçenur
dc.contributor.authorErganiş, Osman
dc.contributor.authorBalevi, Aslı
dc.date.accessioned2025-07-10T12:19:40Z
dc.date.available2025-07-10T12:19:40Z
dc.date.issued2025
dc.departmentVeteriner Fakültesi
dc.description.abstractRhodococcus equi is an important bacterial pathogen and causes severe chronic granulomatous pneumonia in foals below 6 months of age. It has also become an opportunistic and emerging pathogen in immunocompromised humans. Vaccination is the most cost-effective strategy for controlling and preventing this infection. Although several potential virulence genes and candidate immunogens have been identified over the years, no effective vaccine is currently available to prevent R. equi disease in horses. Recently, bacterial vector vaccines have been shown to be promising for R. equi. In this study, the virulence-associated protein A (VapA) gene of R. equi was cloned into Protein Expression System small ubiquitin-related modifier (pET-SUMO) expression vectors and transferred into Escherichia coli BL21 (DE3). Also, adjuvant significantly affects the efficacy of recombinant vaccines. Therefore, native VapA and recombinant VapA were formulated with Immunostimuling Microparticle System (IMS 3012) or PetGel A (recommended for horses) and subcutaneously administered to mice. The immunization effect of four different vaccines was determined by assaying antibody titers and survival rates. The antibody response was slightly higher in the PetGel A formulations than IMS 3012. Survival rates were lower in the PetGel A formulations than IMS 3012. Given these results, recombinant VapA adjuvanted with PetGel A represents a promising formulation for developing new-generation R. equi vaccines.
dc.identifier.doi10.30466/vrf.2024.2028438.4266
dc.identifier.issn20088140
dc.identifier.issue5
dc.identifier.scopus105006856180
dc.identifier.urihttps://dx.doi.org/10.30466/vrf.2024.2028438.4266
dc.identifier.urihttps://hdl.handle.net/20.500.12451/13242
dc.identifier.volume16
dc.identifier.wosWOS:001490693300001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakScopus
dc.indekslendigikaynakWeb of Science
dc.institutionauthorSanioğlu Gölen, Gokcenur
dc.language.isoen
dc.publisherUrmia University - Faculty of Veterinary Medicine
dc.relation.ispartofVeterinary Research Forum
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectIMS 3012
dc.subjectPetGel A
dc.subjectRecombinant VapA
dc.subjectRhodococcus equi
dc.subjectVaccine
dc.titleComparison of the efficacies of Rhodococcus equi recombinant vaccine in mice
dc.typeArticle

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