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Investigation of the Correlation Between ELISA and Serum Amyloid A in the Diagnosis of Bordetella bronchiseptica in Dogs
(John Wiley and Sons Inc, 2025) Sanioğlu Gölen, Gökçenur; Akar, Kadir; Ekin, İsmail Hakkı
Background: Bordetella bronchiseptica is an essential bacterial pathogen characterized by chronic respiratory disease in dogs known as Kennel cough. The presence of causative antibodies in animals can also be detected by lipopolysaccharide antigen-based enzyme linked immunosorbent assay (ELISA). In recent years, it has been determined that there is a significant relationship between acute phase proteins and diseases, and disease follow-up can be done within the framework of this relationship. Methods: In this study, blood sera from 150 dogs in an animal shelter in Van province were evaluated for B. bronchiseptica by the homemade ELISA method, and their correlations with serum amyloid A (SAA) were investigated. Blood serum samples were analysed for antibodies against B. bronchiseptica using a homemade ELISA method. Positive animals were also molecularly confirmed using nasal swabs by PCR. A commercial ELISA kit determined SAA levels in blood sera. Results: Eighteen (12%) of the analysed blood serum samples were found positive by the homemade ELISA method. SAA concentrations in the positive blood sera were elevated from 12.7 to ≤38.98 mg/L. SAA concentrations in blood sera serologically positive for B. bronchiseptica were statistically significant. Conclusions: In this study, in which the relationship between SAA concentration and B. bronchiseptica was investigated for the first time in Turkey, it was concluded that SAA concentration analysis may help diagnose and monitor the disease. In addition, the presence and prevalence of this critical and zoonotic agent causing chronic respiratory tract disease in dogs in Van province was revealed for the first time in this study.
Investigation of the effect of water and feed sourced boron on the growth performance and blood parameters of Nile tilapia, Oreochromis niloticus
(John Wiley and Sons Inc, 2024) Çelik, Mustafa; Dikel, Suat; Öz, Mustafa
This study investigated the effects of water and feed-derived boron on the growth performance and blood parameters of Nile tilapia fingerlings. Two different experiments were designed for this purpose. The first phase of the study determined the LC50 (96-h) value of boron for Nile tilapia. Fish were then fed in water containing boron at a ratio of 1:20 of the LC50 value. In the second experiment, feed containing boron at different rates (0.00%, 0.01%, 0.05%, and 0.10%) was fed for 40 days. At the end of the feeding period, growth performance, hematology, and blood biochemistry parameters were determined. The study concluded with a calculated LC50 value of 161.053 mg/L boron for Nile tilapia. The initial weight was 12.51 ± 0.79 g; at the end of feeding, the final weights were determined as 26.36 ± 0.15 g for the control and 28.07 ± 0.23, 32.28 ± 0.25 and 24.81 ± 0.48 g for 0.01%, 0.05% and 0.10% of boron feed treatments, respectively. At the end of feeding in water containing boron, the final weight of Nile tilapia was determined as 24.26 ± 0.26 g (LC50/20%). The results showed that feeding Nile tilapia with 0.05% boron-supplemented feed stimulated growth and positively affected blood parameters, whereas waterborne boron inhibited Nile tilapia growth and negatively affected blood parameters.
An immunohistochemical study on the evaluation of mast cell, interleukin 17 and interleukin 1? profile in contagious caprine pleuropneumonia
(Urmia University - Faculty of Veterinary Medicine, 2024) Karataş , Özhan; Akçakavak, Gökhan
Contagious caprine pleuropneumonia (CCPP) in goats is defined as a highly contagious and rapidly spreading mycoplasmal disease that is now among the leading causes of major economic losses on many continents (Asia, Africa and the Middle East). In this study, we aimed to evaluate immunohistochemically mast cells (MCs) profile and local interleukin (IL)-17 and IL-1? protein expressions in naturally infected CCPP according to the course of the inflammation (peracute-acute, subacute-chronic). The material of the study consisted of 40 naturally infected CCPP and 6 healthy control goat lung tissues. Appropriate samples were taken from the necropsied goats and subjected to histopathological and immunohistochemical examination. In the histopathological examination of the samples, it was determined that 29 samples had a peracute-acute course and 11 had a subacute-chronic course. In immuno-histochemical examination, MC profile and local IL-17 and IL-1? protein expressions were evaluated in the peracute-acute and subacute-chronic course. Immunohistochemically, significant increases in MC number, local IL-17 and IL-1? scores were detected in the peracute-acute course compared to the control group. There were significant decreases in the relevant scores in the subacute-chronic course compared to the peracute-acute course. Current findings indicated that MC, IL-17, and IL-1? expressions played important roles in the pathogenesis of infection in naturally infected CCPP, especially in the peracute-acute course.
A molecular and histopathological study on bronchopneumonia in cats
(Kırgızistan-Türkiye Manas Üniversitesi, 2024) Akçakavak, Gökhan; Tuzcu, Nevin; Çelik, Zeynep; Tural, Ayşenur; Dağar, Osman; Tuzcu, Mehmet
In this study, it was aimed to determine Bordetella bronchiseptica, Mycoplasma felis, Staphylococcus aureus and Chlamydia felis, which cause bronchopneumonia in cats, by Real-time PCR and to compare the pathological findings of the identified agents. The material of the study was constituted of paraffin blocks belonging to the lungs, of which 21 bronchopneumonia were detected in microscopic examination (with Hematoxylin Eosin (HE)) from a total of 78 cats samples brought to Selcuk University, Faculty of Veterinary Medicine, Department of Pathology for pathological diagnosis. Histopathologically, polymorphonuclear leukocytes (PMNL) and mononuclear cell infiltration (MCI) in the bronchi and bronchiolar lumens, desquamation in the bronchi and bronchiolar epithelium, PMNL infiltration with oedama in alveolar lumens and desquamated alveolar epithelium, PMNL infiltration in the interstitium, and peribronchi and peribronchiolar MCI, and pleuritis were detected. Real-time PCR analysis revealed Bordetella bronchiseptica in 3 (14.29%) cases, Mycoplasma felis in 3 (14.29%), Staphylococcus aureus in 5 (23.8%), and Chlamydia felis in 5 (23.8%). Morever, Mycoplasma felis and Staphylococcus aureus infection was detected in 1 case, and Staphylococcus aureus and Chlamydia felis mixed infection was observed in 1 case. Our results show that relevant agents can frequently be isolated in cases of feline bronchopneumonia.
Determination of local expressions of IGF-1, LC3B and NF-kB in white muscle disease in lambs by immunohistochemical method
(İlker ÇAMKERTEN, 2024) Akçakavak, Gökhan; Karataş, Özhan; Tural, Ayşenur; Dağar, Osman; Doğan, Osman; Tuzcu, Mehmet
White muscle disease (WMD) is also known as Stiff Lamb Disease or Nutritional Muscular Dystrophy. Selenium and/or Vitamin E deficiency constitutes the etiology of the disease. This study aimed to immunohistochemically evaluate local protein expressions of Nuclear factor kappa B (NF-kB), Insulin-like growth factor-1 (IGF-1) and Microtubule-related protein 1A/1B-light chain 3 beta (LC3B) in WMD. The material of the study consisted of 15 WMD, and 6 healthy lamb heart samples. The heart tissues of the autopsied lambs were subjected to routine tissue processing and paraffin blocks were obtained. Then, it was stained with Hematoxylin-Eosin and immunohistochemical methods. Control group lambs had normal macroscopic appearance. Macroscopically, hyaline degeneration and zenker’s necrosis, calcification areas were observed in WMD tissues. Microscopically, degenerative and necrotic muscle fibers, calcification areas, fibrosis, mononuclear cell infiltrates and macrophage infiltrates were detected in WMD heart tissues. Immunohistochemically, significant increases were detected in IGF-1 (p<0.001), LC3B (p<0.001) and NF-kB (p<0.05) in the WMD group compared to the control group. Immunoreactivity in the relevant primers was detected commonly in degenerative and necrotic muscle fibers. In addition, occasional immunoreactivity was observed in the relevant primers in inflammatory cell infiltrates. In conclusion, NF-kB, IGF-1 and LC3B protein expressions were evaluated immunohistochemically for the first time in lambs with WMD. Our findings show that IGF-1 and LC3B proteins are highly expressed in heart tissue in WMD. Additionally, it is possible to say that IGF-1 and LC3B can be used in the diagnosis of WMD.
Development of a simple and rapid DNA extraction method for aspergillus Flavus-A
(Sciendo, 2024) Sanio?lu Gölen, Gökçenur; Akar, Kadir
Aspergillus species are known to be very important in human and domestic animal health. Aspergillus species commonly cause severe systemic and skin infections, as well as allergic lung diseases. With the development of PCR techniques, these methods are used to identify and diagnose fungi. DNA extraction from Aspergillus species is difficult because the fungal cell wall structure is very durable and complex. Fungal DNA extraction methods containing proteinase K and liquid nitrogen are widely used to break down the cell wall. However, these methods cause DNA loss during the extraction in Aspergillus species. In this study, on the contrary, the commonly used DNA extraction by means of ammonium hydroxide, which is generally used to break down chitin in DNA extraction of ticks and plants, is used. The efficiency of the cell wall lysis method from A. flavus with ammonium hydroxide was compared with methods containing proteinase K and liquid nitrogen. For this purpose, DNA extraction of A. flavus was tried using three different methods. As a result, the cell wall of A. flavus was lysed using ammonium hydroxide in this study. The obtained DNA's quality, concentration, and PCR performance were sufficient. This method has been evaluated as a faster, more straightforward, and more economical alternative.
Multifunctional hernia repair biopatch: Development, characterization, in vitro and in vivo evaluation
(Editions de Sante, 2024) Deveci, Mehmet Zeki Yılmaz; Enguven, Gözde; Ege, Hasan; Alakuş, İbrahim; Ağtürk, Gökhan; Dal Yöntem, Fulya; Yılmaz, Şenanur; Kırgız, Ömer; Akçakavak, Gökhan; Kazak, Filiz; Aksu, Burak; Alakuş, Halil
Incisional hernia, a prevalent postoperative complication, is characterized by the protrusion of organs or tissues through damaged abdominal wall. Predisposing factors for hernias include obesity, wound infections, immunosuppression, and comorbidities. However, hernia patches currently in use, including the commercial polypropylene (PP) patch, still have limitations in providing the mechanical and biological properties necessary for abdominal wall regeneration. In this study, three dimensional (3D) printing and coaxial electrospinning methods were combined to create a multifunctional double layered hernia repair biopatch to overcome these limitations. The double-layer design of the biopatch serves a multifunctional role in addressing incisional hernia models, with 3D printed ciprofloxacin (CIP) loaded polycaprolactone (PCL)/gelatin (Ge) scaffold (3DCIP) layer and coaxially electrospun PCL/Ge/?-carrageenan (?-C) nanofiber (NF) layers. While 3DCIP layers provides temporary mechanical reinforcement to the damaged abdominal wall, prevention of adhesions to internal organs, and reduction of surgical site infections, NF layer serves as tissue regeneration and fast wound healing. The developed multifunctional hernia biopatches underwent comprehensive physical and chemical characterization, followed by in vitro and in vivo evaluations. These evaluations included comparisons with a commercial PP patch, which was used as the control in the experiments. The study successfully fabricated multifunctional hernia biopatches with excellent antibacterial properties, high mechanical robustness, and strong biocompatibility.
45S5 bioactive glass-Ointment positively effects on wound healing in rats by regulating TNF?, Il-10, VEGF, and TGF?
(Wiley, 2024) Kırgız, Ömer; Altuğ, Muhammed Enes; Özkan, Hüseyin; Han, Mehmet Cengiz; Akçakavak, Gökhan; Özarslan, Ali Can; Yücel, Sevil
This study aimed to investigate the effects of 45S5 bioactive glass-ointment (BG) on cutaneous wound healing in rats at the molecular, biochemical, and histopathological levels.Materials and MethodsThirty-two rats were divided into four groups (n = 8): Control, Sham, BG, and DEX (Dexpanthenol). While no wound treatment was applied to the CONTROL, a wound model was created in the Sham, and no treatment was applied. A wound model was created for other groups, and BG and DEX were applied locally for 21 days. During the 21-day experiment period, feed and water consumption and weight changes were observed. Wound areas were calculated on days 0, 3, 7, 4, and 21. Following treatment, the rats were euthanized and tissues from the wound area and blood samples were collected. While the expression levels of tumor necrosis factor-alpha (TNF alpha), Interleukin 6 (IL6), Interleukin 10 (IL10), transforming growth factor-beta (TGF beta), and vascular endothelial growth factor (VEGF) genes were determined by qPCR, the levels of TNF alpha, IL6, and IL10 proteins were measured by ELISA.ResultsIt was observed that the BG group showed anti-inflammatory activity by suppressing TNF alpha levels and stimulating IL-10. In addition, it was determined that BG increased fibroblast activity and vascularization.ConclusionCurrent findings showed that topical application of BG has anti-inflammatory effects, while also accelerating healing by increasing vascularity and making positive contributions to tissue healing.
Effect of GnRH administration on different days following embryo transfer on conception rates in beef heifers
(Herman Otto Intezet, 2024) Satılmış, Fatma; Erdem, Hüseyin; Karaşahin, Tahir; Aalkan, Hasan; Dursun, Şükrü; Güler, Mehmet
This study investigated the effect of GnRH, administered on days 4 and 5 after the transfer of fresh embryos to beef heifers, on the conception rate. For this purpo-se, thirty Simmental cows were used as donors in the study. Progesterone-based protocol (eleven-day) was applied to the donors for estrus synchronization. In the synchronization of the recipients, the classical ovsynch protocol was applied. Uterine flushing was performed on the 7th day following artificial insemination of the donors. Code I and II quality fresh embryos were transferred to 117 recipient heifers suitable for transfer (?15 mm CL). Recipient heifers were divided into three groups; GnRH-4, GnRH-5, and control. After the embryo transfer intramuscular GnRH (10 ?g, Buserelin Acetate) was administered to the recipients on the 11th and 12th days of the cycle (4 and 5 days following the transfer). No administration was performed on heifers in the control group. On day 23 following the transfer, pregnancy examinations were performed with real-time ultrasound. Consequently, the conception rates of GnRH-4, GnRH-5, and control groups were 38.7%, 43.3%, and 33.9%, respectively (p > 0.05). As a result, GnRH administration on the 4th and 5th days following the transfer did not increase significantly the pregnancy rate. Since the pregnancy rates obtained in a high-cost biotechnological method such as embryo transfer are very important, the post-transfer GnRH administration can be recommended because there is a statistically insignificant numerical increase (approximately 10%) in the presented study. However, this strategy should be applied to a larger number of animals and be molecularly and endocrinologically evaluated.
Investigation of ivermectin susceptibility in Kangal and Akbaş Dogs via MDR1 gene mutation
(Harran Üniversitesi, 2024) Baydan, Emine; Çınar Kul, Bengi; Akkurt, Mustafa Yenal; Yıldırım, Ebru; Arslanbaş, Emre; Gönül Aydıni Farah
This research aimed to investigate the sensitivity to the drug (ivermectin) in Kangal and Akbaş breed dogs, which are dog breeds native to Turkey, via the MDR1 gene mutation. For the research, blood, hair and intraoral swap samples were taken from 30 Kangal and 20 Akbaş breed dogs (male-female mixed, adult) with ethical permission and approval forms. Kangal dog samples were taken from the dogs bred in the farms in Sivas center and Kangal village, and Akbaş dog samples were taken from the dogs bred in the farms in the Sivrihisar center, Ankara and Eskişehir regions. The samples taken were evaluated based on polymerase chain reaction (PCR) from the wool and subsequent two-way dideoxy chain termination reaction in the presence of 4 base farm deletions (c.296-299delAGAT) in the 4th exon of the ABCB1 gene that encodes the P-glycoprotein (P-gp) drug transporter protein. According to the obtained DNA sequence results, the deletion of “AGAT” was not determined in any of the individuals screened. The results of this study, which is preliminary research, showed that Kangal and Akbaş breed dogs are safe in terms of sensitivity to drugs that are set to be absorbed and excreted by the P-gp pump, especially ivermectin. However, it would be useful to repeat the analysis of both breeds with more examples.
Investigation of the presence and prevalence of listeriosis in clinical samples in Van and its region
(Harran Üniversitesi, 2024) Akar, Kadir; Sanioğlu Gölen, Gökçenur; Babaoğlu, Ali Rıza; Ekin, İbrahim Hakkı
Listeriosis is an infection that causes abortion in humans and various animals worldwide. The causative agent is spread by livestock faeces, especially ruminants, and has a zoonotic character, transmitted by ingesting contaminated silage and feed. In this study, it was aimed to investigate the presence of Listeria spp. in 120 samples (79 abortion material, 41 brain material) of sheep. For this purpose, the polymerase chain reaction (PCR) method was performed using specific oligonucleotide pairs for Listeria spp. all of the abortion materials from Van province and its districts were found to be negative. However, a total of 2 (4.87%) samples, one each from the Erciş and Gevaş districts, from sheep with clinical nervous symptoms were found positive. As a result, it was determined that Listeria spp. was sporadic in Van province. It was concluded that this situation may be due to the low use of silage in ovine breeding in Van province. It was thought that periodical studies should be carried out to determine the course of the disease in the region.
Determination of Apoptosis, Necroptosis and Autophagy Markers by Real-time PCR in Naturally Infected Pneumonic Pasteurellosis caused by Pasteurella multocida and Mannheimia haemolytica in Cattle
(University of Agriculture, 2024) Akçakavak, Gökhan; Karataş, Özhan; Tuzcu, Nevin; Tuzcu, Mehmet
Pneumonic pasteurellosis (PP) is defined as one of the pivotal infectious diseases caused by Pasteurella multocida and Mannheimia haemolytica. This study aimed to determine the levels of Bcl-2-associated protein X (Bax), B-cell lymphoma 2 (Bcl-2), caspase-3, autophagy related-5 (Atg5), beclin-1 and receptor interacting protein-3 (RIP3) in lung tissues with naturally infected PP caused by P. multocida and M. haemolytica, and to reveal their effects on the pathogenesis of P. multocida and M. haemolytica pneumonia. The material of the study consisted of 150 fibrinous pneumonia/pleuropneumonia and 10 healthy lung tissue samples. Relevant samples were examined by histopathological, immunohistochemical and real-time PCR methods. Immunohistochemically, 23 (15.3%) were positive for P. multocida, and 17 (11.3%) were positive for M. haemolytica. Subsequently, the processes of apoptosis, autophagy and necroptosis for P. multocida and M. haemolytica were evaluated by real-time PCR. P. multocida pneumonia increased Bax, Caspase-3, Atg5, Beclin-1, and RIP3 gene expressions (4.2, 3.8, 2.9, 2.1, 2.8-fold, respectively), whereas Bcl-2 gene expression was decreased (0.22-fold). While Bax, Caspase-3, Atg5, Beclin-1, and RIP3 gene expressions were increased in M. haemolytica pneumonia (2.3, 1.9, 1.7, 1.2, 4.2-fold, respectively), it was observed that Bcl-2 gene expression was reduced (0.52-fold). The results obtained in the study revealed the importance of necroptosis, apoptosis and autophagy processes in the pathogenesis of PP caused by P. multocida and M. haemolytica and contributed to the literature. In addition, we found that the processes of apoptosis and autophagy play a more active role in PP caused by P. multocida, and the process of necroptosis plays a more active role in PP caused by M. haemolytica.
Proanthocyanidin alleviates testicular torsion/detorsion-induced ischemia/reperfusion injury in rats
(Elsevier, 2024) Kazak, Filiz; Akçakavak, Gökhan; Alakuş, İbrahim; Alakuş, Halil; Kırgız, Ömer; Karataş, Özhan; Deveci, Mehmet Zeki Yılmaz; Coşkun, Pınar
Testicular torsion is an urological emergency and can lead to ischemia damage and testicular loss if not diagnosed in time. Proanthocyanidin is reported to have anti-inflammatory and antioxidant properties. The current study aimed to examine the possible effects of proanthocyanidin (P) on the testis in torsion/detorsion (T/D)-induced testicular ischemia/reperfusion (I/R) injury in rats. Forty rats were divided into four groups (n=10 for each): sham-operated (sham), I/R, I/R + P100 (100?mg/kg, 30?min before torsion), and I/R + P200 (200?mg/kg, 30?min before torsion). Testicular T/D was performed on the left testicle by 3?hours of torsion at 720° clockwise, followed by 3?hours of detorsion. In the I/R group, an increase in malondialdehyde (MDA) levels and a decrease in glutathione (GSH), vitamin C (Vit C), glutathione peroxidase (GPx), glucose-6-phosphate dehydrogenase (G6PD) values were determined compared to the sham group (p<0.001). Moreover, an increase in the expression of cleaved caspase-3 and Bcl2-associated X protein (Bax), a decrease in the expression of B-cell lymphoma 2 (Bcl-2) and proliferating cell nuclear antigen (PCNA) were detected in the I/R group (p<0.001). Histopathologically, it was determined that the Johnsen and Cosentino scores of the testicles were irregular in the I/R group (p<0.001). Proanthocyanidin treatment caused a decrease in MDA, cleaved caspase-3 and Bax levels and an increase in GSH, Vit C, GPx, G6PD, Bcl-2 and PCNA values. Additionally, Johnsen and Cosentino rearranged the scores. The present findings revealed the protective and curative effects of proanthocyanidin in organ damage due to testicular torsion/detorsion-induced ischemia/reperfusion with their antioxidative and antiapoptotic properties.
Tarantula cubensis alcohol extract enhances the tumoricidal effect of capecitabine via multiple pathways in azoxymethane-induced colorectal cancer in rats
(University of Benin, 2024) Akçakavak, Gökhan; Çelik, Zeynep; Karataş, Özhan; Doğan, Osman; Özdemir, Özgür; Tuzcu, Mehmet
To evaluate the effect of a combination of Tarantula cubensis alcohol extract (TCAE) and capecitabine (CAP) in the treatment of azoxymethane (AOM)-induced colorectal cancer (CRC). Methods: Forty-two Wistar albino rats were divided into 7 groups with 6 rats in each group. The groups consisted of Control (C), Control+TCAE (C-TCAE), Control+CAP (C-CAP), Cancer control (CC), Cancer+TCAE (CC-TCAE), Cancer+CAP (CC-CAP) and Cancer+CAP+TCAE (CC-CAP+TCAE). To induce CRC, AOM (15 mg/kg) was administered to rats subcutaneously (sc) twice at a one-week interval to all the groups except control. From the 15th week, TCAE (0.2 mL/rat sc) was administered to CC-TCAE group every 3 days for 4 weeks, and CAP (40 mg/kg/day) was administered by gavage to CC-CAP group for 4 weeks. In CC-CAP+TCAE group, TCAE (0.2 mL/rat sc) was administered every 3 days for 4 weeks, and CAP (40 mg/kg/day) was administered gavage for 4 weeks. Animals were treated for 18 weeks. Aberrant crypt foci (ACF) were evaluated histopathologically among CC, CC-TCAE, CC-CAP, and CC-CAP+TCAE groups. ?-catenin, CD15, Proliferating Cell Nuclear Antigen (PCNA), and Nuclear Factor kappa B (NF-?B) expression levels were immunohistochemically compared among all groups. Results: Histopathologically, ACF scores were significantly increased in CC group, while a significant decrease in the relevant scores (p < 0.001) was observed in CC-CAP and CC-CAP+TCAE treatment groups, and the lowest scores were in CC-CAP+TCAE group. Immunohistochemically, in CC group, ?-catenin, Nuclear Factor kappa B (NF-?B), Proliferating Cell Nuclear Antigen (PCNA) and CD15 expressions were highly irregular. CC-CAP and CC-CAP+TCAE groups had significantly reduced expressions (p < 0.001), and the lowest expressions were in CC-CAP+TCAE group. Conclusion: The combined use of TCAE and CAP in treatment of CRC has a synergistic effect and increases the anticancer efficacy of TCAE, and CAP. More studies at the molecular level are needed in the future to demonstrate the clinical benefit of TCAE supplementation during the treatment of CRC with CAP.
Effects of dietary garlic (Allium sativum) oil on growth performance, haemato-biochemical and histopathology of cypermethrin-intoxicated Nile tilapia (Oreochromis niloticus)
(John Wiley and Sons Inc, 2024) Öz, Mustafa; İnanan, Burak Evren; Üstüner, Enes; Karagöz, Betül; Dikel, Suat
When pesticides are introduced into wetlands by agriculture, fish quickly absorb them through their gills. Pesticides reduce hatchability, impede growth, and antioxidant response, killing fish. Therefore, it's crucial to find effective pesticide mitigation methods for fish. Objective: In this study, the effects of garlic (Allium sativum) oil on the growth, haematology, biochemistry and histopathology parameters of Nile tilapia (Oreochromis niloticus) exposed to cypermethrin toxicity were investigated. Methods: In the research, cypermethrin was added to the water of the experimental groups at a rate of 1:20 of the LC50 value, and 1.00% garlic oil was added to the fish feed. Fish with an initial weight of 30.26 ± 0.26 g were fed for 45 days. Results: At the end of feeding, the final weights were determined as 69.39 ± 0.41 (G1), 61.81 ± 0.65 (G2), 82.25 ± 0.36 (G3), and 75.04 ± 0.68 (G4) grams, respectively. Histopathological examinations revealed serious lesions in the gill, liver, brain, and muscle tissues in the cypermethrin group, whereas these lesions were minimal or absent in the garlic oil group. Conclusions: Garlic oil supplementation had positive effects on growth, haematology, blood biochemistry, hepatosomatic index and histopathological parameters. These findings suggest that garlic oil is a potential protective agent against cypermethrin toxicity.
Cumin (Cuminum cyminum L.) seeds accelerates wound healing in rats: Possible molecular mechanisms
(John Wiley and Sons Inc, 2024) Ahmed, Khaled Abdul-Aziz; Jabbar, Ahmed A. J.; Galali, Yaseen; M. Al-Qaaneh, Ayman; Akçakavak, Gökhan; Salehen, Nur Ain; Hassan, Rawaz Rizgar; Mothana, Ramzi A.
Wound healing is a complex, intricate, and dynamic process that requires effective therapeutic management. The current study evaluates the wound healing potentials of methanolic extract of Cuminum cyminum L. seeds (CCS) in rats. Sprague Dawley (24) rats were distributed into four cages, wounds produced on the back of the neck, and received two daily topical treatments for 14 days: A, rats received normal saline; B, wounded rats treated with intrasite gel; C and D, rats received 0.2 mL of 250 and 500 mg/kg of CCS, respectively. After that, wound area and closure percentage were evaluated, and wound tissues were dissected for histopathological, immunohistochemical, and biochemical examinations. Acute toxicity trials of methanolic extract of CCS showed the absence of any physiological changes or mortality in rats. CCS application caused a significant reduction in wound size and a statistically elevated percentage of wound contraction than those of vehicle rats. CCS treatment caused significant up-regulation of collagen fiber, fibroblasts, and fewer inflammatory cells (inflammation) in granulation tissues. TGF-?1 (angiogenetic factor) was significantly more expressed in CCS-treated rats in comparison to normal saline-treated rats; therefore, more fibroblasts transformed into myofibroblasts (angiogenesis). CCS-treated rats showed remarkable antioxidant potentials (higher SOD and CAT enzymes) and decreased MDA (lipid peroxidation) levels in their wound tissue homogenates. Hydroxyproline amino acid (collagen) was significantly up-regulated by CCS treatment, which is commonly related to faster wound closure area. The outcomes suggest CCS as a viable new source of pharmaceuticals for wound treatment.
Determining the protective effect of a boron adjuvanted vaccine with an experimental infection model in mice
(TÜBİTAK, 2023) Sayın, Zafer; Uslu, Ali; Özdemir, Özgür; Başoğlu, Abdullah; Balevi, Aslı; Uçan, Uçkun Sait; Aras, Zeki; Erganiş, Osman
Boron is a required trace element for plants, humans, and animals and it has significant influence over biological functions and the immune system. Vaccines have aided humans in the control of many contagious diseases around the world. The success of global immunization campaigns may be possible with the development of effective and low-cost vaccines and adjuvants. For this reason, the present study aimed to determine the adjuvant activity of easily accessible boron compounds. In this study, six different vaccines were prepared, including inactive Staphylococcus aureus bacteria, boric acid, aluminum hydroxide, montanide ISA 50, and ISA 206, ISA 50 + boric acid, and ISA 206 + boric acid combinations. The rate of survival following the challenge, the bacterial load on internal organs, and histopathological findings at the vaccine injection site and in the internal organs were evaluated in vaccinated mice. In addition, the levels of interferon - ? and tumor necrosis factor - ? were measured in the vaccinated seropotency groups. The rate of postchallenge mortality was 50% in the control group, and no mortality was encountered in the boric acid group. The boric acid adjuvanted vaccine decreased the bacterial load and postchallenge abscess in the internal organs and also local inflammatory reactions due to montanide adjuvants in combinations. No difference was found in the interferon - ? and tumor necrosis factor - ? levels between the control and vaccine groups. It was concluded that boric acid can be used as an adjuvant in inactivated vaccines.
Investigation of quality parameters of common carp (Cyprinus carpio) meat marinated with traditional method used in Anatolia during storage at ?18 °C
(AZTI-Tecnalia, 2023) Öz, Mustafa; Uçak, İlknur
Improving the sensory quality of fish meat is crucial aspect to enhance fish consumption. In this study, the cooking methods in some Anatolian cities where common carp is consumed very popularly were investigated, and carp meat was marinated using different ratios of lemon juice (0.00%, 40.00%, 50.00%, and 60.00%) and salt (10.00%) using the traditional method. After marinating, the effects on the sensory, chemical, and microbiological quality of carp fillets stored at ?18 °C were investigated. As a result of the research, it was observed that lemon had positive effects on the shelf life of fish meat. Peroxide value (PV), pH value, Thiobarbituric Acid (TBARS), the total number of psychrophilic bacteria, and the total number of yeast and molds were lower in the groups marinated with lemon juice and salt compared to the control group. In addition, it was observed that the panelists liked the carp meat of the groups marinated with lemon juice.
Molecular cloning of the OMP19 gene from Brucella melitensis strain H38 and its antigenicity compared to that of commercial OMP19
(PAN, 2022) Uslu, A.; Sanioğlu Gönen, Gökçenur; Tekindal, M. Agah; Sakmanoğlu, A.; Sayın, Z.; Denizli, O.; Gök, A.; Erganiş, Osman
Brucellosis is a worldwide zoonosis, that can still be classified as endemic despite its ancient origins which causes economic losses and public health problems. Although effectively controlled by vaccination in animals, there is currently no vaccine for use in humans. Outer Membrane Proteins (OMP) that play an active immunogenic and protective role in the Brucellae family. OMP19 is present in all Brucella species as a surface antigen and is a potent immunogen responsible for Brucellosis intracellular infection. For this reason, the study was aimed to be used safely as a potential recombinant vaccine candidate against all Brucella infections, especially in humans and pregnant animals. This study evaluated a Brucella lipoprotein antigen, i.e. 19 kilodalton (kDa) outer membrane protein (OMP19), which was amplified and cloned into the pETSUMO vector system. The immunogenic power of the purified recombinant OMP19 antigen against brucellosis was compared with that of OMP19 (Raybiotech Inc, USA) in a mouse model and the obtained rOMP19 antigen was found to be similar to the commercially available recombinant protein.
Expression pattern of microRNAs in ovine endometrium during the peri-implantation
(Elsevier Inc., 2022) Köse, Mehmet; Hitit, Mustafa; Kaya, Mehmet Salih; Kırbaş, Mesut; Dursun, Şükrü; Alak, İlyas; Atlı, Mehmet Osman
MicroRNA (miRNA), acting as the transcriptional regulator of gene expression, has been widely demonstrated to be involved in many biological functions, including embryo implantation and development. The objective of the current study was to illuminate the expression pattern of microRNAs (miRNAs) in the endometrium during the peri-implantation in ewes. Intercaruncular endometrial samples was obtained from a total of 24 ewes on days of 12 (pre-implantation, n = 4), 16 (implantation, n = 4) and 22 (post-implantation, n = 4) of pregnancy following mating, and on their corresponding days of 12 (n = 4), 16 (n = 4) and 22 (n = 4) of the estrous cycle. The miRNA profiles were examined in the endometrium by microarray technology. We detected 116 ovine specifics miRNAs in the endometrium. Of these, nineteen were differentially expressed in early pregnancy. Four miRNAs (oar-miR-370-3p, oar-miR-411b-5p, oar-miR-379-3p and oar-miR-411a-3p) that had the most differential fold change were confirmed by RT-qPCR in ovine endometrium. The differentially expressed miRNAs targeted a total of 315 genes, resulting in 39 GO terms in molecular function, 353 in biological process, and 17 in the cellular component. The construction of the PPI network of target genes established two functional modules mostly enriched in the innate immune system, toll receptor cascades in module 1, whereas genes in module 2 were associated with GMCSF-mediated signaling events, insulin pathway, and mTOR signaling pathway. Based on the results, we may imply that miRNAs modulate ovine endometrium during the peri-implantation.

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