Detergent compatible extracellular lipase from streptomyces cellulosae AU-10: A green alternative for the detergent industry

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dc.authoridBoran, Rukiye -- 0000-0003-2395-2445
dc.contributor.authorBoran, Rukiye
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:17:00Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:17:00Z
dc.date.issued2018
dc.departmentSağlık Hizmetleri Meslek Yüksekokulu
dc.description.abstractEnzymes can decrease the environmental and economic load of detergent products by reducing the amount of chemicals used in detergents and by allowing washing at ambient temperatures. In this study, Streptomyces cellulosae AU-10 (GenBank accession number: MG780240) lipase was purified 7.08-fold with 68% yield using an aqueous 2-phase system. The Streptomyces sp. AU-10 lipase showed maximal activity at pH 9.0 and 40 degrees C. Hundred percent activities were measured in the pH range from 9.0 to 11.0 for 1 h. The enzyme was also highly stable at 30-50 degrees C. The values of K-m and V-max were calculated as 0.34mM and 0.83mMmin(-1), respectively. The lipase has high hydrolytic activity for olive oil and sunflower oil. The effect of ethylenediamine tetraacetic acid on the enzyme has shown that the lipase is a metalloenzyme. The activity increased in the presence of Fe2+, Cu2+, and various boron compounds. The enzyme has shown a good stability not only with surfactants but also with oxidizing agents. In addition, activities in the presence of Omo, Ariel, Tursil, Pril, and Fairy were measured as 108.8%, 115.6%, 98.35%, 140.4%, and 107.6%, respectively. Considering its remarkable ability, the S. cellulosae AU-10 lipase can be considered as a potential additive in the detergent industry.
dc.identifier.doi10.1002/jsde.12049
dc.identifier.endpage573en_US
dc.identifier.issn1097-3958
dc.identifier.issn1558-9293
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage565en_US
dc.identifier.urihttps://doi.org/10.1002/jsde.12049
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4687
dc.identifier.volume21en_US
dc.identifier.wosWOS:000435278300012
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWıley
dc.relation.ispartofJournal of Surfactants and Detergents
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAlkaline Lipase
dc.subjectStreptomyces Cellulosae
dc.subjectBiochemical Characterization
dc.subjectDetergent Industry
dc.titleDetergent compatible extracellular lipase from streptomyces cellulosae AU-10: A green alternative for the detergent industry
dc.typeArticle

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